Maritalea myrionectae
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3695 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R4ME96|A0A2R4ME96_9RHIZ Uncharacterized protein OS=Maritalea myrionectae OX=454601 GN=MXMO3_01690 PE=4 SV=1
MM1 pKa = 7.87 AYY3 pKa = 8.6 ATTNPPNVVAGDD15 pKa = 3.51 VGTNSVWVYY24 pKa = 10.84 ASEE27 pKa = 5.87 DD28 pKa = 2.94 IHH30 pKa = 7.66 TDD32 pKa = 2.77 VDD34 pKa = 3.44 ATDD37 pKa = 3.65 YY38 pKa = 10.98 FSNGDD43 pKa = 3.69 ALGMRR48 pKa = 11.84 VNDD51 pKa = 3.22 VVIVVKK57 pKa = 8.26 TTATIGATIHH67 pKa = 6.04 VVTAVTTDD75 pKa = 3.4 GAATISPAILAA86 pKa = 4.52
Molecular weight: 8.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.617
ProMoST 4.062
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.884
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.808
Nozaki 4.012
DTASelect 4.317
Thurlkill 3.719
EMBOSS 3.884
Sillero 3.973
Patrickios 1.952
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|A0A2R4MB16|A0A2R4MB16_9RHIZ Protein QmcA OS=Maritalea myrionectae OX=454601 GN=MXMO3_00705 PE=4 SV=1
MM1 pKa = 7.76 RR2 pKa = 11.84 SIFILIALLAFGPLSIWLDD21 pKa = 3.26 SDD23 pKa = 3.89 LVSDD27 pKa = 4.45 FRR29 pKa = 11.84 LRR31 pKa = 11.84 SEE33 pKa = 4.02 KK34 pKa = 10.43 LVYY37 pKa = 10.77 ADD39 pKa = 3.95 VPTSQRR45 pKa = 11.84 KK46 pKa = 7.65 CTGTPVLLYY55 pKa = 10.1 SCGFKK60 pKa = 10.84 FEE62 pKa = 4.68 IAGEE66 pKa = 4.41 TYY68 pKa = 9.67 RR69 pKa = 11.84 KK70 pKa = 9.98 RR71 pKa = 11.84 YY72 pKa = 9.68 VLVALGAPKK81 pKa = 9.55 TVHH84 pKa = 6.25 LLKK87 pKa = 10.84 GEE89 pKa = 3.92 QSGVITSTVGQDD101 pKa = 3.66 YY102 pKa = 9.98 IWNRR106 pKa = 11.84 IIAVLLACAVSLTTAIRR123 pKa = 11.84 LFRR126 pKa = 11.84 VFARR130 pKa = 11.84 PKK132 pKa = 9.78 ANLRR136 pKa = 11.84 FAPEE140 pKa = 4.16 TPSDD144 pKa = 3.83 RR145 pKa = 11.84 SHH147 pKa = 6.04 STATGQRR154 pKa = 11.84 AQHH157 pKa = 5.4 RR158 pKa = 11.84 TGNAGFGPRR167 pKa = 11.84 SNGTFGRR174 pKa = 11.84 RR175 pKa = 3.16
Molecular weight: 19.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.692
IPC_protein 10.496
Toseland 10.555
ProMoST 10.467
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.877
Grimsley 10.745
Solomon 10.789
Lehninger 10.745
Nozaki 10.555
DTASelect 10.409
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.599
IPC_peptide 10.789
IPC2_peptide 9.531
IPC2.peptide.svr19 8.573
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3695
0
3695
1120000
30
7755
303.1
33.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.695 ± 0.043
0.844 ± 0.014
6.081 ± 0.055
6.239 ± 0.044
4.24 ± 0.033
7.825 ± 0.042
2.146 ± 0.021
6.021 ± 0.032
4.484 ± 0.039
9.892 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.77 ± 0.024
3.511 ± 0.023
4.478 ± 0.027
3.826 ± 0.03
5.42 ± 0.034
5.568 ± 0.033
5.22 ± 0.03
7.031 ± 0.034
1.245 ± 0.016
2.463 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here