Myoviridae sp.
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 545 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345MMX9|A0A345MMX9_9CAUD Erythromycin resistance protein OS=Myoviridae sp. OX=2202564 PE=4 SV=1
MM1 pKa = 7.66 LFIPKK6 pKa = 9.92 RR7 pKa = 11.84 NTEE10 pKa = 4.35 GMVSMIKK17 pKa = 9.69 MSVKK21 pKa = 8.76 ITEE24 pKa = 4.34 IEE26 pKa = 3.72 WDD28 pKa = 3.89 FDD30 pKa = 3.77 DD31 pKa = 4.9 EE32 pKa = 4.84 TYY34 pKa = 11.19 QDD36 pKa = 3.36 IEE38 pKa = 4.48 GVNLHH43 pKa = 6.39 LPSVPFYY50 pKa = 10.02 VTVEE54 pKa = 4.28 AEE56 pKa = 5.34 DD57 pKa = 4.98 DD58 pKa = 5.06 DD59 pKa = 5.21 IDD61 pKa = 4.06 NLPVADD67 pKa = 5.73 AISDD71 pKa = 3.71 EE72 pKa = 4.41 YY73 pKa = 10.61 GWCVKK78 pKa = 10.32 NCEE81 pKa = 3.97 YY82 pKa = 10.79 DD83 pKa = 3.18 IEE85 pKa = 4.62 TVLVV89 pKa = 3.66
Molecular weight: 10.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.757
IPC_protein 3.706
Toseland 3.503
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.63
Rodwell 3.541
Grimsley 3.427
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.643
Sillero 3.834
Patrickios 1.85
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.76
Protein with the highest isoelectric point:
>tr|A0A345MM35|A0A345MM35_9CAUD Uncharacterized protein OS=Myoviridae sp. OX=2202564 PE=4 SV=1
MM1 pKa = 7.62 RR2 pKa = 11.84 ALYY5 pKa = 9.8 HH6 pKa = 5.92 VSITIDD12 pKa = 2.79 RR13 pKa = 11.84 RR14 pKa = 11.84 KK15 pKa = 9.79 IKK17 pKa = 10.45 RR18 pKa = 11.84 QINKK22 pKa = 8.83 IQKK25 pKa = 9.57 KK26 pKa = 9.98 DD27 pKa = 3.34 SKK29 pKa = 10.57 LSKK32 pKa = 9.94 KK33 pKa = 10.29 RR34 pKa = 11.84 RR35 pKa = 11.84 DD36 pKa = 3.33 ISNHH40 pKa = 3.8 YY41 pKa = 10.62 VFFEE45 pKa = 4.19 FTTNLYY51 pKa = 10.92 NIFCIFIKK59 pKa = 10.4 RR60 pKa = 11.84 SNSKK64 pKa = 9.27 IHH66 pKa = 5.88 YY67 pKa = 5.67 QTKK70 pKa = 10.17 AYY72 pKa = 9.69 RR73 pKa = 11.84 NNYY76 pKa = 8.55 LRR78 pKa = 11.84 LICNIIYY85 pKa = 9.02 NQRR88 pKa = 11.84 FLTYY92 pKa = 8.71 SRR94 pKa = 11.84 KK95 pKa = 10.13 YY96 pKa = 10.34 KK97 pKa = 10.56 VSQSKK102 pKa = 10.78 NKK104 pKa = 10.19 IITLEE109 pKa = 3.9
Molecular weight: 13.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.293
IPC2_protein 10.087
IPC_protein 10.54
Toseland 10.657
ProMoST 10.292
Dawson 10.789
Bjellqvist 10.452
Wikipedia 10.965
Rodwell 11.257
Grimsley 10.847
Solomon 10.833
Lehninger 10.804
Nozaki 10.628
DTASelect 10.452
Thurlkill 10.657
EMBOSS 11.038
Sillero 10.701
Patrickios 10.965
IPC_peptide 10.833
IPC2_peptide 9.297
IPC2.peptide.svr19 8.332
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
545
0
545
119766
80
5045
219.8
25.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.458 ± 0.116
1.292 ± 0.055
6.543 ± 0.12
6.129 ± 0.234
4.594 ± 0.083
5.272 ± 0.178
1.581 ± 0.07
9.897 ± 0.114
8.482 ± 0.266
7.325 ± 0.111
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.378 ± 0.072
8.566 ± 0.225
3.003 ± 0.07
2.614 ± 0.054
2.85 ± 0.103
7.734 ± 0.226
6.319 ± 0.215
5.29 ± 0.09
1.033 ± 0.052
5.64 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here