Amino acid dipepetide frequency for Myoviridae sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.079AlaAla: 2.079 ± 0.201
0.526AlaCys: 0.526 ± 0.068
1.862AlaAsp: 1.862 ± 0.149
1.854AlaGlu: 1.854 ± 0.161
1.361AlaPhe: 1.361 ± 0.105
2.196AlaGly: 2.196 ± 0.211
0.543AlaHis: 0.543 ± 0.069
3.131AlaIle: 3.131 ± 0.185
2.63AlaLys: 2.63 ± 0.178
2.588AlaLeu: 2.588 ± 0.2
0.793AlaMet: 0.793 ± 0.091
2.38AlaAsn: 2.38 ± 0.184
1.328AlaPro: 1.328 ± 0.138
0.969AlaGln: 0.969 ± 0.101
1.378AlaArg: 1.378 ± 0.116
2.697AlaSer: 2.697 ± 0.181
2.296AlaThr: 2.296 ± 0.194
1.887AlaVal: 1.887 ± 0.179
0.426AlaTrp: 0.426 ± 0.069
1.662AlaTyr: 1.662 ± 0.129
0.0AlaXaa: 0.0 ± 0.0
Cys
0.376CysAla: 0.376 ± 0.056
0.25CysCys: 0.25 ± 0.049
0.835CysAsp: 0.835 ± 0.078
0.802CysGlu: 0.802 ± 0.083
0.66CysPhe: 0.66 ± 0.08
0.743CysGly: 0.743 ± 0.079
0.309CysHis: 0.309 ± 0.049
1.144CysIle: 1.144 ± 0.113
1.186CysLys: 1.186 ± 0.102
0.902CysLeu: 0.902 ± 0.089
0.317CysMet: 0.317 ± 0.058
1.186CysAsn: 1.186 ± 0.101
0.409CysPro: 0.409 ± 0.054
0.259CysGln: 0.259 ± 0.046
0.359CysArg: 0.359 ± 0.057
0.969CysSer: 0.969 ± 0.098
0.61CysThr: 0.61 ± 0.071
0.685CysVal: 0.685 ± 0.068
0.092CysTrp: 0.092 ± 0.028
0.827CysTyr: 0.827 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
2.062AspAla: 2.062 ± 0.135
0.726AspCys: 0.726 ± 0.08
4.592AspAsp: 4.592 ± 0.218
4.509AspGlu: 4.509 ± 0.232
3.348AspPhe: 3.348 ± 0.229
3.874AspGly: 3.874 ± 0.211
0.852AspHis: 0.852 ± 0.088
7.999AspIle: 7.999 ± 0.291
4.885AspLys: 4.885 ± 0.251
4.183AspLeu: 4.183 ± 0.184
1.845AspMet: 1.845 ± 0.127
5.402AspAsn: 5.402 ± 0.233
1.57AspPro: 1.57 ± 0.127
1.394AspGln: 1.394 ± 0.107
1.728AspArg: 1.728 ± 0.128
4.693AspSer: 4.693 ± 0.249
4.108AspThr: 4.108 ± 0.207
3.256AspVal: 3.256 ± 0.165
0.785AspTrp: 0.785 ± 0.088
3.615AspTyr: 3.615 ± 0.226
0.0AspXaa: 0.0 ± 0.0
Glu
2.196GluAla: 2.196 ± 0.216
0.777GluCys: 0.777 ± 0.086
3.524GluAsp: 3.524 ± 0.245
4.734GluGlu: 4.734 ± 0.343
2.956GluPhe: 2.956 ± 0.184
2.639GluGly: 2.639 ± 0.173
0.994GluHis: 0.994 ± 0.097
5.728GluIle: 5.728 ± 0.241
5.97GluLys: 5.97 ± 0.367
5.118GluLeu: 5.118 ± 0.249
1.47GluMet: 1.47 ± 0.122
6.037GluAsn: 6.037 ± 0.233
1.219GluPro: 1.219 ± 0.115
1.962GluGln: 1.962 ± 0.133
1.645GluArg: 1.645 ± 0.142
2.939GluSer: 2.939 ± 0.192
3.507GluThr: 3.507 ± 0.212
3.023GluVal: 3.023 ± 0.165
0.935GluTrp: 0.935 ± 0.106
3.924GluTyr: 3.924 ± 0.219
0.0GluXaa: 0.0 ± 0.0
Phe
1.411PheAla: 1.411 ± 0.125
0.827PheCys: 0.827 ± 0.083
3.006PheAsp: 3.006 ± 0.187
2.497PheGlu: 2.497 ± 0.186
1.945PhePhe: 1.945 ± 0.175
2.446PheGly: 2.446 ± 0.212
0.735PheHis: 0.735 ± 0.077
4.91PheIle: 4.91 ± 0.252
3.473PheLys: 3.473 ± 0.209
3.265PheLeu: 3.265 ± 0.202
1.119PheMet: 1.119 ± 0.094
4.283PheAsn: 4.283 ± 0.2
1.211PhePro: 1.211 ± 0.098
0.927PheGln: 0.927 ± 0.09
1.444PheArg: 1.444 ± 0.121
4.1PheSer: 4.1 ± 0.185
2.68PheThr: 2.68 ± 0.168
2.321PheVal: 2.321 ± 0.147
0.384PheTrp: 0.384 ± 0.052
2.956PheTyr: 2.956 ± 0.191
0.0PheXaa: 0.0 ± 0.0
Gly
2.405GlyAla: 2.405 ± 0.254
0.559GlyCys: 0.559 ± 0.07
3.156GlyAsp: 3.156 ± 0.189
2.947GlyGlu: 2.947 ± 0.177
2.313GlyPhe: 2.313 ± 0.153
3.582GlyGly: 3.582 ± 0.369
0.818GlyHis: 0.818 ± 0.088
4.876GlyIle: 4.876 ± 0.205
4.701GlyLys: 4.701 ± 0.212
3.348GlyLeu: 3.348 ± 0.194
1.319GlyMet: 1.319 ± 0.093
4.325GlyAsn: 4.325 ± 0.405
0.985GlyPro: 0.985 ± 0.095
1.269GlyGln: 1.269 ± 0.133
1.603GlyArg: 1.603 ± 0.128
3.883GlySer: 3.883 ± 0.369
3.999GlyThr: 3.999 ± 0.35
2.747GlyVal: 2.747 ± 0.158
0.743GlyTrp: 0.743 ± 0.081
3.139GlyTyr: 3.139 ± 0.199
0.0GlyXaa: 0.0 ± 0.0
His
0.643HisAla: 0.643 ± 0.07
0.242HisCys: 0.242 ± 0.048
1.019HisAsp: 1.019 ± 0.101
0.91HisGlu: 0.91 ± 0.11
0.944HisPhe: 0.944 ± 0.096
0.96HisGly: 0.96 ± 0.083
0.309HisHis: 0.309 ± 0.067
1.92HisIle: 1.92 ± 0.107
1.353HisLys: 1.353 ± 0.128
0.969HisLeu: 0.969 ± 0.105
0.392HisMet: 0.392 ± 0.056
1.219HisAsn: 1.219 ± 0.107
0.543HisPro: 0.543 ± 0.069
0.351HisGln: 0.351 ± 0.057
0.534HisArg: 0.534 ± 0.069
0.96HisSer: 0.96 ± 0.09
0.76HisThr: 0.76 ± 0.085
0.81HisVal: 0.81 ± 0.087
0.159HisTrp: 0.159 ± 0.038
0.818HisTyr: 0.818 ± 0.089
0.0HisXaa: 0.0 ± 0.0
Ile
2.922IleAla: 2.922 ± 0.175
1.261IleCys: 1.261 ± 0.114
7.565IleAsp: 7.565 ± 0.287
6.838IleGlu: 6.838 ± 0.321
4.133IlePhe: 4.133 ± 0.186
4.542IleGly: 4.542 ± 0.216
1.478IleHis: 1.478 ± 0.114
9.627IleIle: 9.627 ± 0.366
8.917IleLys: 8.917 ± 0.363
7.623IleLeu: 7.623 ± 0.279
2.171IleMet: 2.171 ± 0.137
8.124IleAsn: 8.124 ± 0.302
3.54IlePro: 3.54 ± 0.189
2.438IleGln: 2.438 ± 0.17
2.388IleArg: 2.388 ± 0.189
8.876IleSer: 8.876 ± 0.32
6.221IleThr: 6.221 ± 0.265
4.91IleVal: 4.91 ± 0.215
0.726IleTrp: 0.726 ± 0.08
4.667IleTyr: 4.667 ± 0.197
0.0IleXaa: 0.0 ± 0.0
Lys
2.572LysAla: 2.572 ± 0.177
1.052LysCys: 1.052 ± 0.1
4.86LysAsp: 4.86 ± 0.248
5.736LysGlu: 5.736 ± 0.392
3.974LysPhe: 3.974 ± 0.233
3.073LysGly: 3.073 ± 0.186
1.611LysHis: 1.611 ± 0.154
8.943LysIle: 8.943 ± 0.363
8.634LysLys: 8.634 ± 0.511
7.44LysLeu: 7.44 ± 0.342
2.171LysMet: 2.171 ± 0.155
8.408LysAsn: 8.408 ± 0.317
2.229LysPro: 2.229 ± 0.163
2.655LysGln: 2.655 ± 0.142
2.371LysArg: 2.371 ± 0.188
5.419LysSer: 5.419 ± 0.197
4.484LysThr: 4.484 ± 0.191
3.64LysVal: 3.64 ± 0.201
0.86LysTrp: 0.86 ± 0.097
4.684LysTyr: 4.684 ± 0.229
0.0LysXaa: 0.0 ± 0.0
Leu
2.538LeuAla: 2.538 ± 0.175
1.269LeuCys: 1.269 ± 0.095
4.534LeuAsp: 4.534 ± 0.198
4.4LeuGlu: 4.4 ± 0.269
3.114LeuPhe: 3.114 ± 0.185
3.398LeuGly: 3.398 ± 0.186
1.269LeuHis: 1.269 ± 0.103
5.786LeuIle: 5.786 ± 0.219
6.688LeuLys: 6.688 ± 0.321
5.653LeuLeu: 5.653 ± 0.298
1.72LeuMet: 1.72 ± 0.129
4.993LeuAsn: 4.993 ± 0.188
2.981LeuPro: 2.981 ± 0.189
2.271LeuGln: 2.271 ± 0.145
2.296LeuArg: 2.296 ± 0.162
6.538LeuSer: 6.538 ± 0.247
4.684LeuThr: 4.684 ± 0.226
4.058LeuVal: 4.058 ± 0.184
0.651LeuTrp: 0.651 ± 0.082
4.409LeuTyr: 4.409 ± 0.194
0.0LeuXaa: 0.0 ± 0.0
Met
1.01MetAla: 1.01 ± 0.099
0.309MetCys: 0.309 ± 0.052
1.528MetAsp: 1.528 ± 0.114
1.637MetGlu: 1.637 ± 0.15
1.269MetPhe: 1.269 ± 0.106
0.96MetGly: 0.96 ± 0.104
0.342MetHis: 0.342 ± 0.047
2.138MetIle: 2.138 ± 0.139
2.338MetLys: 2.338 ± 0.171
1.695MetLeu: 1.695 ± 0.12
0.559MetMet: 0.559 ± 0.069
1.82MetAsn: 1.82 ± 0.124
0.526MetPro: 0.526 ± 0.072
0.576MetGln: 0.576 ± 0.072
0.584MetArg: 0.584 ± 0.072
1.945MetSer: 1.945 ± 0.132
1.536MetThr: 1.536 ± 0.13
1.653MetVal: 1.653 ± 0.13
0.184MetTrp: 0.184 ± 0.041
1.169MetTyr: 1.169 ± 0.104
0.0MetXaa: 0.0 ± 0.0
Asn
2.263AsnAla: 2.263 ± 0.161
0.918AsnCys: 0.918 ± 0.093
5.052AsnAsp: 5.052 ± 0.226
4.267AsnGlu: 4.267 ± 0.201
4.016AsnPhe: 4.016 ± 0.168
5.027AsnGly: 5.027 ± 0.446
1.202AsnHis: 1.202 ± 0.115
10.253AsnIle: 10.253 ± 0.365
7.364AsnLys: 7.364 ± 0.283
6.028AsnLeu: 6.028 ± 0.282
1.954AsnMet: 1.954 ± 0.139
8.592AsnAsn: 8.592 ± 0.479
2.739AsnPro: 2.739 ± 0.145
2.188AsnGln: 2.188 ± 0.126
2.288AsnArg: 2.288 ± 0.152
6.429AsnSer: 6.429 ± 0.361
5.561AsnThr: 5.561 ± 0.434
4.233AsnVal: 4.233 ± 0.235
0.693AsnTrp: 0.693 ± 0.087
4.592AsnTyr: 4.592 ± 0.311
0.0AsnXaa: 0.0 ± 0.0
Pro
1.094ProAla: 1.094 ± 0.109
0.326ProCys: 0.326 ± 0.058
2.087ProAsp: 2.087 ± 0.132
2.421ProGlu: 2.421 ± 0.164
1.378ProPhe: 1.378 ± 0.109
1.069ProGly: 1.069 ± 0.111
0.426ProHis: 0.426 ± 0.068
2.355ProIle: 2.355 ± 0.157
2.179ProLys: 2.179 ± 0.146
2.129ProLeu: 2.129 ± 0.131
0.61ProMet: 0.61 ± 0.068
2.246ProAsn: 2.246 ± 0.143
0.91ProPro: 0.91 ± 0.104
0.827ProGln: 0.827 ± 0.093
0.877ProArg: 0.877 ± 0.087
2.58ProSer: 2.58 ± 0.172
2.396ProThr: 2.396 ± 0.189
2.121ProVal: 2.121 ± 0.143
0.317ProTrp: 0.317 ± 0.053
1.678ProTyr: 1.678 ± 0.111
0.0ProXaa: 0.0 ± 0.0
Gln
1.202GlnAla: 1.202 ± 0.1
0.284GlnCys: 0.284 ± 0.048
1.278GlnAsp: 1.278 ± 0.102
1.511GlnGlu: 1.511 ± 0.107
1.278GlnPhe: 1.278 ± 0.1
1.403GlnGly: 1.403 ± 0.119
0.434GlnHis: 0.434 ± 0.07
2.138GlnIle: 2.138 ± 0.123
2.179GlnLys: 2.179 ± 0.12
2.505GlnLeu: 2.505 ± 0.149
0.593GlnMet: 0.593 ± 0.061
2.179GlnAsn: 2.179 ± 0.177
0.768GlnPro: 0.768 ± 0.078
0.902GlnGln: 0.902 ± 0.081
0.86GlnArg: 0.86 ± 0.103
1.854GlnSer: 1.854 ± 0.143
1.428GlnThr: 1.428 ± 0.102
1.202GlnVal: 1.202 ± 0.092
0.292GlnTrp: 0.292 ± 0.044
1.854GlnTyr: 1.854 ± 0.126
0.0GlnXaa: 0.0 ± 0.0
Arg
1.152ArgAla: 1.152 ± 0.116
0.376ArgCys: 0.376 ± 0.058
1.678ArgAsp: 1.678 ± 0.124
1.804ArgGlu: 1.804 ± 0.147
1.328ArgPhe: 1.328 ± 0.115
1.695ArgGly: 1.695 ± 0.118
0.551ArgHis: 0.551 ± 0.081
2.38ArgIle: 2.38 ± 0.148
2.63ArgLys: 2.63 ± 0.186
1.962ArgLeu: 1.962 ± 0.129
0.86ArgMet: 0.86 ± 0.089
2.405ArgAsn: 2.405 ± 0.156
0.893ArgPro: 0.893 ± 0.089
0.86ArgGln: 0.86 ± 0.097
1.236ArgArg: 1.236 ± 0.117
1.795ArgSer: 1.795 ± 0.142
1.303ArgThr: 1.303 ± 0.1
1.52ArgVal: 1.52 ± 0.12
0.367ArgTrp: 0.367 ± 0.055
1.703ArgTyr: 1.703 ± 0.126
0.0ArgXaa: 0.0 ± 0.0
Ser
2.463SerAla: 2.463 ± 0.17
0.843SerCys: 0.843 ± 0.081
5.569SerAsp: 5.569 ± 0.244
4.025SerGlu: 4.025 ± 0.234
3.615SerPhe: 3.615 ± 0.183
4.726SerGly: 4.726 ± 0.344
1.094SerHis: 1.094 ± 0.114
7.64SerIle: 7.64 ± 0.316
5.669SerLys: 5.669 ± 0.264
5.611SerLeu: 5.611 ± 0.239
1.804SerMet: 1.804 ± 0.141
6.655SerAsn: 6.655 ± 0.468
2.305SerPro: 2.305 ± 0.156
1.854SerGln: 1.854 ± 0.126
1.854SerArg: 1.854 ± 0.135
6.822SerSer: 6.822 ± 0.456
5.419SerThr: 5.419 ± 0.446
4.15SerVal: 4.15 ± 0.21
0.676SerTrp: 0.676 ± 0.081
4.542SerTyr: 4.542 ± 0.366
0.0SerXaa: 0.0 ± 0.0
Thr
2.313ThrAla: 2.313 ± 0.212
0.71ThrCys: 0.71 ± 0.083
4.392ThrAsp: 4.392 ± 0.254
3.357ThrGlu: 3.357 ± 0.213
2.831ThrPhe: 2.831 ± 0.164
3.782ThrGly: 3.782 ± 0.337
0.918ThrHis: 0.918 ± 0.087
6.062ThrIle: 6.062 ± 0.251
4.275ThrLys: 4.275 ± 0.174
4.793ThrLeu: 4.793 ± 0.245
1.127ThrMet: 1.127 ± 0.094
4.96ThrAsn: 4.96 ± 0.395
2.488ThrPro: 2.488 ± 0.134
1.728ThrGln: 1.728 ± 0.163
1.628ThrArg: 1.628 ± 0.118
5.252ThrSer: 5.252 ± 0.372
4.893ThrThr: 4.893 ± 0.434
3.674ThrVal: 3.674 ± 0.197
0.643ThrTrp: 0.643 ± 0.068
3.365ThrTyr: 3.365 ± 0.222
0.0ThrXaa: 0.0 ± 0.0
Val
1.895ValAla: 1.895 ± 0.134
0.685ValCys: 0.685 ± 0.087
3.774ValAsp: 3.774 ± 0.175
2.989ValGlu: 2.989 ± 0.183
2.43ValPhe: 2.43 ± 0.142
2.805ValGly: 2.805 ± 0.152
0.852ValHis: 0.852 ± 0.098
4.843ValIle: 4.843 ± 0.177
4.45ValLys: 4.45 ± 0.235
3.382ValLeu: 3.382 ± 0.157
1.311ValMet: 1.311 ± 0.11
4.116ValAsn: 4.116 ± 0.168
1.87ValPro: 1.87 ± 0.136
1.144ValGln: 1.144 ± 0.103
1.444ValArg: 1.444 ± 0.125
4.5ValSer: 4.5 ± 0.283
3.507ValThr: 3.507 ± 0.195
3.223ValVal: 3.223 ± 0.181
0.726ValTrp: 0.726 ± 0.077
2.956ValTyr: 2.956 ± 0.187
0.0ValXaa: 0.0 ± 0.0
Trp
0.292TrpAla: 0.292 ± 0.052
0.142TrpCys: 0.142 ± 0.032
0.751TrpAsp: 0.751 ± 0.091
0.726TrpGlu: 0.726 ± 0.077
0.543TrpPhe: 0.543 ± 0.061
0.568TrpGly: 0.568 ± 0.069
0.184TrpHis: 0.184 ± 0.037
0.86TrpIle: 0.86 ± 0.083
1.002TrpLys: 1.002 ± 0.085
0.827TrpLeu: 0.827 ± 0.073
0.175TrpMet: 0.175 ± 0.037
0.777TrpAsn: 0.777 ± 0.084
0.184TrpPro: 0.184 ± 0.046
0.259TrpGln: 0.259 ± 0.05
0.292TrpArg: 0.292 ± 0.057
0.743TrpSer: 0.743 ± 0.076
0.61TrpThr: 0.61 ± 0.074
0.777TrpVal: 0.777 ± 0.076
0.109TrpTrp: 0.109 ± 0.031
0.509TrpTyr: 0.509 ± 0.076
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.695TyrAla: 1.695 ± 0.133
0.835TyrCys: 0.835 ± 0.09
4.367TyrAsp: 4.367 ± 0.243
3.332TyrGlu: 3.332 ± 0.174
2.505TyrPhe: 2.505 ± 0.142
3.231TyrGly: 3.231 ± 0.175
0.893TyrHis: 0.893 ± 0.095
6.237TyrIle: 6.237 ± 0.335
4.292TyrLys: 4.292 ± 0.183
3.231TyrLeu: 3.231 ± 0.148
1.461TyrMet: 1.461 ± 0.115
5.553TyrAsn: 5.553 ± 0.259
1.328TyrPro: 1.328 ± 0.101
1.311TyrGln: 1.311 ± 0.11
1.687TyrArg: 1.687 ± 0.113
4.35TyrSer: 4.35 ± 0.291
3.19TyrThr: 3.19 ± 0.241
3.014TyrVal: 3.014 ± 0.178
0.559TyrTrp: 0.559 ± 0.077
3.332TyrTyr: 3.332 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 545 proteins (119766 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski