Cuneatibacter caecimuris
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3170 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q7PPV9|A0A4Q7PPV9_9FIRM Uncharacterized protein OS=Cuneatibacter caecimuris OX=1796618 GN=EV209_1097 PE=4 SV=1
MM1 pKa = 7.81 RR2 pKa = 11.84 KK3 pKa = 8.98 ILKK6 pKa = 9.77 KK7 pKa = 10.41 CAALGLGAVMLLSVAGCGLGQDD29 pKa = 4.6 DD30 pKa = 4.94 SVLPTFNVSADD41 pKa = 3.64 QLSDD45 pKa = 4.47 LDD47 pKa = 5.53 LKK49 pKa = 11.1 DD50 pKa = 5.57 LYY52 pKa = 11.04 DD53 pKa = 3.75 EE54 pKa = 4.15 QLGYY58 pKa = 10.31 SYY60 pKa = 11.15 KK61 pKa = 10.65 GVSYY65 pKa = 10.95 DD66 pKa = 3.89 LDD68 pKa = 3.99 VEE70 pKa = 4.74 GVGAALGVDD79 pKa = 3.4 MGDD82 pKa = 3.32 SRR84 pKa = 11.84 KK85 pKa = 10.43 LGGTEE90 pKa = 3.77 YY91 pKa = 10.21 TCYY94 pKa = 10.42 YY95 pKa = 8.1 PQEE98 pKa = 4.17 AATTVNGQVPVTMEE112 pKa = 3.8 FVFGGDD118 pKa = 2.94 KK119 pKa = 10.53 KK120 pKa = 10.78 FRR122 pKa = 11.84 SATYY126 pKa = 10.73 QFMANGNGMNAEE138 pKa = 4.11 SLDD141 pKa = 3.66 ALFDD145 pKa = 3.52 QTLDD149 pKa = 3.65 QLHH152 pKa = 6.25 EE153 pKa = 4.41 LYY155 pKa = 10.88 GKK157 pKa = 7.97 EE158 pKa = 4.04 TEE160 pKa = 4.38 LRR162 pKa = 11.84 EE163 pKa = 4.21 SSDD166 pKa = 3.34 EE167 pKa = 4.27 SGSNTLDD174 pKa = 3.06 SVTYY178 pKa = 10.14 NWKK181 pKa = 9.84 QDD183 pKa = 3.54 MNDD186 pKa = 3.61 GYY188 pKa = 9.39 ITSLQLVKK196 pKa = 10.73 GDD198 pKa = 3.75 YY199 pKa = 11.07 GYY201 pKa = 9.15 GTDD204 pKa = 3.74 IVILGVIRR212 pKa = 11.84 YY213 pKa = 6.75 TYY215 pKa = 10.36 EE216 pKa = 5.09 DD217 pKa = 3.57 EE218 pKa = 6.29 AEE220 pKa = 4.19 AQSAEE225 pKa = 4.29 STEE228 pKa = 4.27 EE229 pKa = 3.76
Molecular weight: 24.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.999
IPC_protein 3.986
Toseland 3.783
ProMoST 4.113
Dawson 3.961
Bjellqvist 4.139
Wikipedia 3.872
Rodwell 3.808
Grimsley 3.681
Solomon 3.961
Lehninger 3.91
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.821
EMBOSS 3.884
Sillero 4.101
Patrickios 1.24
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.994
Protein with the highest isoelectric point:
>tr|A0A4Q7P369|A0A4Q7P369_9FIRM Replicative DNA helicase OS=Cuneatibacter caecimuris OX=1796618 GN=EV209_2208 PE=3 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.86 KK9 pKa = 8.2 RR10 pKa = 11.84 SRR12 pKa = 11.84 AKK14 pKa = 9.27 VHH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 KK29 pKa = 8.89 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.71 GRR39 pKa = 11.84 KK40 pKa = 8.73 VLSAA44 pKa = 4.05
Molecular weight: 4.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3170
0
3170
1036976
30
2908
327.1
36.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.163 ± 0.047
1.551 ± 0.019
5.298 ± 0.033
7.835 ± 0.052
3.997 ± 0.031
7.697 ± 0.046
1.686 ± 0.018
6.354 ± 0.041
5.842 ± 0.041
9.499 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.025 ± 0.024
3.825 ± 0.033
3.595 ± 0.023
3.592 ± 0.025
5.251 ± 0.047
5.874 ± 0.038
5.08 ± 0.039
6.797 ± 0.037
1.1 ± 0.016
3.94 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here