Fusarium oxysporum f. sp. cubense (strain race 1) (Panama disease fungus)
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15355 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|N4U470|N4U470_FUSC1 Hexose transporter HXT13 OS=Fusarium oxysporum f. sp. cubense (strain race 1) OX=1229664 GN=FOC1_g10000036 PE=3 SV=1
MM1 pKa = 6.95 VRR3 pKa = 11.84 YY4 pKa = 10.08 SILAVAFAILGAEE17 pKa = 4.45 ASPCRR22 pKa = 11.84 PTTVTSVTEE31 pKa = 4.06 ASSTIASDD39 pKa = 3.64 TTVTALATTVTTALVEE55 pKa = 4.46 TTSTAVAEE63 pKa = 4.51 TTTTTEE69 pKa = 3.68 APACVEE75 pKa = 4.15 TQVVVNPGFDD85 pKa = 3.98 DD86 pKa = 3.79 SDD88 pKa = 4.44 SNKK91 pKa = 10.35 SPWFGDD97 pKa = 3.47 GSLTTDD103 pKa = 4.51 GPNTAPNAISFVFSNGQGSAQLSQTLTNLDD133 pKa = 3.01 GNYY136 pKa = 9.8 RR137 pKa = 11.84 LSYY140 pKa = 9.37 NWAVVSGVNVGAGFSCSITPKK161 pKa = 10.14 VRR163 pKa = 11.84 GDD165 pKa = 3.83 TLPGADD171 pKa = 3.42 PSEE174 pKa = 4.31 FVGWTPEE181 pKa = 3.98 SQTWSSGSEE190 pKa = 3.56 AVAQADD196 pKa = 3.53 LSFVLEE202 pKa = 4.59 CFGEE206 pKa = 4.01 YY207 pKa = 10.29 DD208 pKa = 3.33 QLTINVDD215 pKa = 3.9 DD216 pKa = 3.59 ITFTKK221 pKa = 10.75 LCGPP225 pKa = 4.8
Molecular weight: 23.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.745
IPC_protein 3.706
Toseland 3.503
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.63
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.024
Thurlkill 3.567
EMBOSS 3.643
Sillero 3.834
Patrickios 0.896
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|N4TI04|N4TI04_FUSC1 Uncharacterized protein OS=Fusarium oxysporum f. sp. cubense (strain race 1) OX=1229664 GN=FOC1_g10015191 PE=4 SV=1
MM1 pKa = 7.86 PLTRR5 pKa = 11.84 THH7 pKa = 6.63 RR8 pKa = 11.84 HH9 pKa = 4.04 TAPRR13 pKa = 11.84 RR14 pKa = 11.84 SIFSTRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 APAHH26 pKa = 4.84 SHH28 pKa = 5.33 RR29 pKa = 11.84 HH30 pKa = 4.03 TTTTTTTTTKK40 pKa = 9.5 PRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 GMFGGGAGRR53 pKa = 11.84 RR54 pKa = 11.84 THH56 pKa = 6.46 ATTTAPVHH64 pKa = 4.87 HH65 pKa = 6.63 HH66 pKa = 5.14 QRR68 pKa = 11.84 RR69 pKa = 11.84 PSMKK73 pKa = 10.02 DD74 pKa = 2.95 KK75 pKa = 11.41 VSGALLKK82 pKa = 11.04 LKK84 pKa = 10.68 GSLTRR89 pKa = 11.84 RR90 pKa = 11.84 PGVKK94 pKa = 9.89 AAGTRR99 pKa = 11.84 RR100 pKa = 11.84 MRR102 pKa = 11.84 GTDD105 pKa = 3.02 GRR107 pKa = 11.84 GARR110 pKa = 11.84 HH111 pKa = 5.87 HH112 pKa = 7.23 RR113 pKa = 11.84 YY114 pKa = 9.44
Molecular weight: 12.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.023
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.159
Rodwell 12.296
Grimsley 12.72
Solomon 13.173
Lehninger 13.086
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 12.018
IPC_peptide 13.188
IPC2_peptide 12.164
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15355
0
15355
6888665
23
6825
448.6
49.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.358 ± 0.017
1.32 ± 0.008
5.802 ± 0.014
6.169 ± 0.02
3.835 ± 0.013
6.828 ± 0.019
2.376 ± 0.009
5.198 ± 0.013
5.044 ± 0.018
8.851 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.243 ± 0.007
3.796 ± 0.01
5.837 ± 0.026
3.971 ± 0.014
5.775 ± 0.017
7.992 ± 0.022
6.007 ± 0.025
6.135 ± 0.015
1.579 ± 0.008
2.852 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here