Lactobacillus phage Ldl1
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7DMT0|A0A0A7DMT0_9CAUD HNH endonuclease OS=Lactobacillus phage Ldl1 OX=1552735 GN=LDL_055 PE=4 SV=1
MM1 pKa = 7.43 QSRR4 pKa = 11.84 FKK6 pKa = 10.43 IAKK9 pKa = 8.28 EE10 pKa = 3.71 FNKK13 pKa = 10.17 IIAKK17 pKa = 10.06 ACEE20 pKa = 4.08 DD21 pKa = 3.51 QPLEE25 pKa = 4.39 DD26 pKa = 4.31 FLEE29 pKa = 4.32 EE30 pKa = 4.68 EE31 pKa = 4.39 IEE33 pKa = 4.14 EE34 pKa = 4.54 DD35 pKa = 4.74 CSGLDD40 pKa = 4.04 FDD42 pKa = 6.28 DD43 pKa = 4.96 AAQLYY48 pKa = 10.52 DD49 pKa = 3.97 EE50 pKa = 5.23 YY51 pKa = 11.15 RR52 pKa = 11.84 DD53 pKa = 3.75 EE54 pKa = 4.92 CIEE57 pKa = 3.72 WFNYY61 pKa = 9.19 FFEE64 pKa = 4.62 KK65 pKa = 10.21 TCMSPQEE72 pKa = 4.08 FCVLCDD78 pKa = 3.1 WDD80 pKa = 4.06 YY81 pKa = 12.13 VLDD84 pKa = 4.03 EE85 pKa = 5.09 EE86 pKa = 4.52 NDD88 pKa = 3.35 KK89 pKa = 11.05 FLVMDD94 pKa = 3.81 TDD96 pKa = 3.72 KK97 pKa = 11.75 NKK99 pKa = 10.14 FLLVTSMFEE108 pKa = 4.1 HH109 pKa = 6.29 YY110 pKa = 10.37 CIEE113 pKa = 3.98 IFNNLDD119 pKa = 2.93 VHH121 pKa = 6.97 NIDD124 pKa = 4.25 KK125 pKa = 11.06
Molecular weight: 15.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 3.986
IPC_protein 3.948
Toseland 3.757
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.668
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.215
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.062
Patrickios 1.901
IPC_peptide 3.91
IPC2_peptide 4.05
IPC2.peptide.svr19 3.959
Protein with the highest isoelectric point:
>tr|A0A0A7DML2|A0A0A7DML2_9CAUD HNH endonuclease OS=Lactobacillus phage Ldl1 OX=1552735 GN=LDL_039 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.4 KK3 pKa = 10.3 KK4 pKa = 10.31 IIPILLSSATVLGLMFANKK23 pKa = 9.43 ISAATRR29 pKa = 11.84 EE30 pKa = 4.11 EE31 pKa = 4.29 GVDD34 pKa = 3.38 VSSYY38 pKa = 10.36 QGSSSSYY45 pKa = 10.72 FKK47 pKa = 10.23 TLKK50 pKa = 10.79 NKK52 pKa = 8.8 GAKK55 pKa = 9.21 FVIVKK60 pKa = 10.29 VGGSGGGEE68 pKa = 4.17 GYY70 pKa = 10.53 HH71 pKa = 5.46 YY72 pKa = 10.18 QNPSASMQLANAKK85 pKa = 10.23 VNGLAVGAYY94 pKa = 8.37 FWAEE98 pKa = 4.15 CGSSSSEE105 pKa = 3.86 ALRR108 pKa = 11.84 MAKK111 pKa = 10.24 LAVSDD116 pKa = 4.17 AKK118 pKa = 10.78 RR119 pKa = 11.84 AGLKK123 pKa = 8.66 TSSVIAMDD131 pKa = 4.0 YY132 pKa = 10.57 EE133 pKa = 5.14 AGAYY137 pKa = 9.6 ASQKK141 pKa = 8.01 TANTNAVITFMDD153 pKa = 4.8 HH154 pKa = 6.14 IKK156 pKa = 10.53 KK157 pKa = 10.44 AGYY160 pKa = 9.63 KK161 pKa = 9.32 PLFYY165 pKa = 10.61 SGASFARR172 pKa = 11.84 SNVDD176 pKa = 2.97 TKK178 pKa = 11.54 KK179 pKa = 10.77 LVNKK183 pKa = 9.63 YY184 pKa = 9.94 GKK186 pKa = 9.37 SALWIASYY194 pKa = 8.59 KK195 pKa = 9.42 TMNAQYY201 pKa = 9.87 TPDD204 pKa = 3.51 YY205 pKa = 10.88 NYY207 pKa = 9.75 FPSMDD212 pKa = 4.97 GIGIWQYY219 pKa = 10.96 ADD221 pKa = 2.54 NWKK224 pKa = 10.25 GLNVDD229 pKa = 4.08 GNVQFFKK236 pKa = 10.88 VISNGEE242 pKa = 4.24 VTKK245 pKa = 9.63 KK246 pKa = 9.32 TPVKK250 pKa = 10.06 QANATTNKK258 pKa = 9.83 EE259 pKa = 3.91 NGVKK263 pKa = 10.29 KK264 pKa = 10.37 FPKK267 pKa = 10.35 DD268 pKa = 3.11 NTYY271 pKa = 8.8 TVKK274 pKa = 10.95 NGDD277 pKa = 3.42 SWSSIAKK284 pKa = 10.24 AYY286 pKa = 10.35 GIDD289 pKa = 3.7 VNGLAKK295 pKa = 10.87 LNGATTKK302 pKa = 10.46 TMLHH306 pKa = 7.01 PGQKK310 pKa = 10.12 LKK312 pKa = 10.09 LTGHH316 pKa = 6.29 VSNVATKK323 pKa = 10.2 KK324 pKa = 9.81 PKK326 pKa = 9.26 KK327 pKa = 7.15 TTTKK331 pKa = 9.97 KK332 pKa = 9.44 KK333 pKa = 8.1 TVKK336 pKa = 9.24 KK337 pKa = 9.97 HH338 pKa = 4.23 SWIKK342 pKa = 9.21 KK343 pKa = 8.27 SGYY346 pKa = 9.33 FRR348 pKa = 11.84 LNTTIKK354 pKa = 10.04 LRR356 pKa = 11.84 SGASTSSRR364 pKa = 11.84 VISTLYY370 pKa = 9.66 RR371 pKa = 11.84 GQVIKK376 pKa = 10.59 FDD378 pKa = 3.8 AYY380 pKa = 9.7 KK381 pKa = 10.52 ISGGYY386 pKa = 8.32 VWLRR390 pKa = 11.84 QKK392 pKa = 10.68 RR393 pKa = 11.84 SNGYY397 pKa = 9.03 GSVASGVAKK406 pKa = 10.34 NGKK409 pKa = 9.02 RR410 pKa = 11.84 VSTWGTIFF418 pKa = 3.98
Molecular weight: 45.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.134
IPC2_protein 9.677
IPC_protein 9.56
Toseland 10.335
ProMoST 9.897
Dawson 10.482
Bjellqvist 10.087
Wikipedia 10.599
Rodwell 11.199
Grimsley 10.526
Solomon 10.482
Lehninger 10.452
Nozaki 10.292
DTASelect 10.087
Thurlkill 10.335
EMBOSS 10.716
Sillero 10.379
Patrickios 10.862
IPC_peptide 10.482
IPC2_peptide 8.565
IPC2.peptide.svr19 8.624
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
79
0
79
21614
35
2627
273.6
31.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.389 ± 0.478
0.736 ± 0.12
6.264 ± 0.346
6.838 ± 0.375
4.071 ± 0.27
5.959 ± 0.24
1.712 ± 0.093
6.093 ± 0.207
8.999 ± 0.505
8.346 ± 0.271
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.017 ± 0.114
6.875 ± 0.171
2.42 ± 0.162
4.085 ± 0.192
3.9 ± 0.168
7.065 ± 0.305
6.061 ± 0.383
6.699 ± 0.205
1.189 ± 0.099
4.28 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here