Amino acid dipepetide frequency for Lactobacillus phage Ldl1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.516AlaAla: 3.516 ± 0.776
0.416AlaCys: 0.416 ± 0.167
3.701AlaAsp: 3.701 ± 0.702
4.164AlaGlu: 4.164 ± 0.606
2.036AlaPhe: 2.036 ± 0.394
3.285AlaGly: 3.285 ± 0.622
0.972AlaHis: 0.972 ± 0.198
3.47AlaIle: 3.47 ± 0.452
6.894AlaLys: 6.894 ± 1.178
5.506AlaLeu: 5.506 ± 0.66
1.203AlaMet: 1.203 ± 0.227
4.581AlaAsn: 4.581 ± 0.61
1.388AlaPro: 1.388 ± 0.309
2.73AlaGln: 2.73 ± 0.481
3.378AlaArg: 3.378 ± 0.349
4.118AlaSer: 4.118 ± 0.599
4.303AlaThr: 4.303 ± 0.729
4.442AlaVal: 4.442 ± 0.466
0.787AlaTrp: 0.787 ± 0.218
3.007AlaTyr: 3.007 ± 0.378
0.0AlaXaa: 0.0 ± 0.0
Cys
0.324CysAla: 0.324 ± 0.12
0.093CysCys: 0.093 ± 0.062
0.37CysAsp: 0.37 ± 0.171
0.555CysGlu: 0.555 ± 0.237
0.601CysPhe: 0.601 ± 0.207
0.37CysGly: 0.37 ± 0.132
0.093CysHis: 0.093 ± 0.059
0.324CysIle: 0.324 ± 0.148
0.37CysLys: 0.37 ± 0.13
0.648CysLeu: 0.648 ± 0.227
0.139CysMet: 0.139 ± 0.086
0.185CysAsn: 0.185 ± 0.092
0.093CysPro: 0.093 ± 0.074
0.231CysGln: 0.231 ± 0.191
0.37CysArg: 0.37 ± 0.18
0.555CysSer: 0.555 ± 0.191
0.555CysThr: 0.555 ± 0.177
0.509CysVal: 0.509 ± 0.163
0.278CysTrp: 0.278 ± 0.101
0.694CysTyr: 0.694 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
3.378AspAla: 3.378 ± 0.484
0.416AspCys: 0.416 ± 0.133
4.072AspAsp: 4.072 ± 0.639
4.534AspGlu: 4.534 ± 0.558
2.961AspPhe: 2.961 ± 0.347
4.534AspGly: 4.534 ± 0.497
0.74AspHis: 0.74 ± 0.209
3.748AspIle: 3.748 ± 0.581
4.904AspLys: 4.904 ± 0.447
5.552AspLeu: 5.552 ± 0.658
1.249AspMet: 1.249 ± 0.249
4.303AspAsn: 4.303 ± 0.473
1.296AspPro: 1.296 ± 0.3
1.99AspGln: 1.99 ± 0.28
2.591AspArg: 2.591 ± 0.389
4.257AspSer: 4.257 ± 0.445
3.84AspThr: 3.84 ± 0.501
3.378AspVal: 3.378 ± 0.444
1.434AspTrp: 1.434 ± 0.257
3.47AspTyr: 3.47 ± 0.605
0.0AspXaa: 0.0 ± 0.0
Glu
5.228GluAla: 5.228 ± 0.823
0.463GluCys: 0.463 ± 0.174
4.118GluAsp: 4.118 ± 0.683
6.478GluGlu: 6.478 ± 0.764
2.452GluPhe: 2.452 ± 0.385
2.73GluGly: 2.73 ± 0.386
1.481GluHis: 1.481 ± 0.294
4.904GluIle: 4.904 ± 0.49
6.57GluLys: 6.57 ± 0.686
6.385GluLeu: 6.385 ± 0.591
1.666GluMet: 1.666 ± 0.215
4.673GluAsn: 4.673 ± 0.487
0.833GluPro: 0.833 ± 0.183
3.054GluGln: 3.054 ± 0.401
2.591GluArg: 2.591 ± 0.322
4.118GluSer: 4.118 ± 0.406
3.193GluThr: 3.193 ± 0.347
4.396GluVal: 4.396 ± 0.567
0.925GluTrp: 0.925 ± 0.218
2.128GluTyr: 2.128 ± 0.336
0.0GluXaa: 0.0 ± 0.0
Phe
2.406PheAla: 2.406 ± 0.295
1.018PheCys: 1.018 ± 0.279
2.822PheAsp: 2.822 ± 0.466
3.1PheGlu: 3.1 ± 0.573
1.388PhePhe: 1.388 ± 0.232
3.1PheGly: 3.1 ± 0.457
0.555PheHis: 0.555 ± 0.153
1.897PheIle: 1.897 ± 0.391
2.915PheLys: 2.915 ± 0.367
3.516PheLeu: 3.516 ± 0.597
0.74PheMet: 0.74 ± 0.179
3.655PheAsn: 3.655 ± 0.444
0.879PhePro: 0.879 ± 0.232
1.11PheGln: 1.11 ± 0.185
1.249PheArg: 1.249 ± 0.235
2.73PheSer: 2.73 ± 0.465
1.99PheThr: 1.99 ± 0.343
3.563PheVal: 3.563 ± 0.52
0.694PheTrp: 0.694 ± 0.154
1.388PheTyr: 1.388 ± 0.241
0.0PheXaa: 0.0 ± 0.0
Gly
3.516GlyAla: 3.516 ± 0.562
0.509GlyCys: 0.509 ± 0.129
3.378GlyAsp: 3.378 ± 0.375
2.498GlyGlu: 2.498 ± 0.273
2.498GlyPhe: 2.498 ± 0.368
2.961GlyGly: 2.961 ± 0.549
1.434GlyHis: 1.434 ± 0.22
3.47GlyIle: 3.47 ± 0.436
6.015GlyLys: 6.015 ± 0.57
5.691GlyLeu: 5.691 ± 0.565
1.018GlyMet: 1.018 ± 0.177
3.887GlyAsn: 3.887 ± 0.502
0.416GlyPro: 0.416 ± 0.154
2.822GlyGln: 2.822 ± 0.392
2.869GlyArg: 2.869 ± 0.409
4.766GlySer: 4.766 ± 0.533
3.933GlyThr: 3.933 ± 0.596
4.025GlyVal: 4.025 ± 0.528
0.648GlyTrp: 0.648 ± 0.156
3.239GlyTyr: 3.239 ± 0.394
0.0GlyXaa: 0.0 ± 0.0
His
0.601HisAla: 0.601 ± 0.19
0.278HisCys: 0.278 ± 0.1
1.018HisAsp: 1.018 ± 0.208
1.249HisGlu: 1.249 ± 0.243
0.601HisPhe: 0.601 ± 0.143
1.018HisGly: 1.018 ± 0.197
0.463HisHis: 0.463 ± 0.164
1.666HisIle: 1.666 ± 0.277
1.296HisLys: 1.296 ± 0.22
1.342HisLeu: 1.342 ± 0.248
0.416HisMet: 0.416 ± 0.149
0.787HisAsn: 0.787 ± 0.204
0.555HisPro: 0.555 ± 0.178
0.601HisGln: 0.601 ± 0.158
0.694HisArg: 0.694 ± 0.189
1.388HisSer: 1.388 ± 0.31
0.694HisThr: 0.694 ± 0.24
1.342HisVal: 1.342 ± 0.304
0.231HisTrp: 0.231 ± 0.109
0.879HisTyr: 0.879 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
4.21IleAla: 4.21 ± 0.337
0.463IleCys: 0.463 ± 0.174
3.979IleAsp: 3.979 ± 0.533
4.673IleGlu: 4.673 ± 0.529
2.128IlePhe: 2.128 ± 0.301
2.221IleGly: 2.221 ± 0.324
0.879IleHis: 0.879 ± 0.174
3.701IleIle: 3.701 ± 0.596
5.321IleLys: 5.321 ± 0.462
4.118IleLeu: 4.118 ± 0.583
1.573IleMet: 1.573 ± 0.37
4.951IleAsn: 4.951 ± 0.559
2.036IlePro: 2.036 ± 0.324
2.452IleGln: 2.452 ± 0.345
2.545IleArg: 2.545 ± 0.351
5.506IleSer: 5.506 ± 0.509
3.47IleThr: 3.47 ± 0.63
4.349IleVal: 4.349 ± 0.417
0.648IleTrp: 0.648 ± 0.165
1.712IleTyr: 1.712 ± 0.223
0.0IleXaa: 0.0 ± 0.0
Lys
5.969LysAla: 5.969 ± 1.44
0.185LysCys: 0.185 ± 0.091
5.182LysAsp: 5.182 ± 0.536
6.709LysGlu: 6.709 ± 0.62
3.285LysPhe: 3.285 ± 0.467
4.812LysGly: 4.812 ± 0.444
1.943LysHis: 1.943 ± 0.296
5.922LysIle: 5.922 ± 0.368
8.375LysLys: 8.375 ± 1.373
7.727LysLeu: 7.727 ± 0.538
2.036LysMet: 2.036 ± 0.321
6.57LysAsn: 6.57 ± 0.516
1.897LysPro: 1.897 ± 0.293
4.257LysGln: 4.257 ± 0.544
3.84LysArg: 3.84 ± 0.426
4.997LysSer: 4.997 ± 0.851
6.801LysThr: 6.801 ± 0.722
5.09LysVal: 5.09 ± 0.489
0.509LysTrp: 0.509 ± 0.149
3.887LysTyr: 3.887 ± 0.444
0.0LysXaa: 0.0 ± 0.0
Leu
5.598LeuAla: 5.598 ± 0.776
0.324LeuCys: 0.324 ± 0.144
6.339LeuAsp: 6.339 ± 0.578
5.83LeuGlu: 5.83 ± 0.483
3.239LeuPhe: 3.239 ± 0.564
5.506LeuGly: 5.506 ± 0.411
1.712LeuHis: 1.712 ± 0.268
5.228LeuIle: 5.228 ± 0.543
8.698LeuLys: 8.698 ± 0.804
6.246LeuLeu: 6.246 ± 0.582
1.99LeuMet: 1.99 ± 0.361
5.228LeuAsn: 5.228 ± 0.552
2.313LeuPro: 2.313 ± 0.345
3.794LeuGln: 3.794 ± 0.587
3.239LeuArg: 3.239 ± 0.381
5.228LeuSer: 5.228 ± 0.637
4.349LeuThr: 4.349 ± 0.584
4.997LeuVal: 4.997 ± 0.547
0.787LeuTrp: 0.787 ± 0.21
2.822LeuTyr: 2.822 ± 0.444
0.0LeuXaa: 0.0 ± 0.0
Met
1.481MetAla: 1.481 ± 0.23
0.046MetCys: 0.046 ± 0.054
1.018MetAsp: 1.018 ± 0.282
1.342MetGlu: 1.342 ± 0.242
1.018MetPhe: 1.018 ± 0.217
0.694MetGly: 0.694 ± 0.151
0.231MetHis: 0.231 ± 0.093
1.342MetIle: 1.342 ± 0.226
1.943MetLys: 1.943 ± 0.312
1.573MetLeu: 1.573 ± 0.301
0.324MetMet: 0.324 ± 0.128
1.527MetAsn: 1.527 ± 0.246
0.463MetPro: 0.463 ± 0.15
0.787MetGln: 0.787 ± 0.247
0.833MetArg: 0.833 ± 0.194
1.943MetSer: 1.943 ± 0.237
1.573MetThr: 1.573 ± 0.221
1.018MetVal: 1.018 ± 0.252
0.139MetTrp: 0.139 ± 0.079
0.879MetTyr: 0.879 ± 0.19
0.0MetXaa: 0.0 ± 0.0
Asn
4.904AsnAla: 4.904 ± 0.611
0.463AsnCys: 0.463 ± 0.146
4.164AsnAsp: 4.164 ± 0.395
3.887AsnGlu: 3.887 ± 0.481
2.406AsnPhe: 2.406 ± 0.374
5.598AsnGly: 5.598 ± 0.588
1.388AsnHis: 1.388 ± 0.204
4.025AsnIle: 4.025 ± 0.395
6.339AsnLys: 6.339 ± 0.441
6.616AsnLeu: 6.616 ± 0.518
1.666AsnMet: 1.666 ± 0.269
4.488AsnAsn: 4.488 ± 0.576
2.822AsnPro: 2.822 ± 0.371
2.36AsnGln: 2.36 ± 0.345
2.082AsnArg: 2.082 ± 0.418
4.534AsnSer: 4.534 ± 0.633
3.239AsnThr: 3.239 ± 0.439
4.025AsnVal: 4.025 ± 0.473
0.555AsnTrp: 0.555 ± 0.152
3.146AsnTyr: 3.146 ± 0.346
0.0AsnXaa: 0.0 ± 0.0
Pro
1.758ProAla: 1.758 ± 0.334
0.093ProCys: 0.093 ± 0.078
2.128ProAsp: 2.128 ± 0.391
2.036ProGlu: 2.036 ± 0.392
0.879ProPhe: 0.879 ± 0.241
0.833ProGly: 0.833 ± 0.18
0.231ProHis: 0.231 ± 0.105
1.342ProIle: 1.342 ± 0.211
1.851ProLys: 1.851 ± 0.282
1.758ProLeu: 1.758 ± 0.338
0.509ProMet: 0.509 ± 0.124
1.943ProAsn: 1.943 ± 0.297
0.278ProPro: 0.278 ± 0.101
1.018ProGln: 1.018 ± 0.263
0.972ProArg: 0.972 ± 0.224
2.221ProSer: 2.221 ± 0.356
1.249ProThr: 1.249 ± 0.241
1.434ProVal: 1.434 ± 0.304
0.37ProTrp: 0.37 ± 0.126
1.296ProTyr: 1.296 ± 0.284
0.0ProXaa: 0.0 ± 0.0
Gln
2.637GlnAla: 2.637 ± 0.414
0.185GlnCys: 0.185 ± 0.084
1.897GlnAsp: 1.897 ± 0.382
2.869GlnGlu: 2.869 ± 0.411
1.712GlnPhe: 1.712 ± 0.267
1.897GlnGly: 1.897 ± 0.286
0.787GlnHis: 0.787 ± 0.173
2.73GlnIle: 2.73 ± 0.326
3.424GlnLys: 3.424 ± 0.615
3.794GlnLeu: 3.794 ± 0.49
0.925GlnMet: 0.925 ± 0.208
2.406GlnAsn: 2.406 ± 0.378
1.434GlnPro: 1.434 ± 0.283
2.452GlnGln: 2.452 ± 0.418
1.527GlnArg: 1.527 ± 0.297
2.128GlnSer: 2.128 ± 0.259
3.193GlnThr: 3.193 ± 0.459
2.73GlnVal: 2.73 ± 0.289
0.37GlnTrp: 0.37 ± 0.135
1.758GlnTyr: 1.758 ± 0.257
0.0GlnXaa: 0.0 ± 0.0
Arg
1.712ArgAla: 1.712 ± 0.29
0.185ArgCys: 0.185 ± 0.105
1.99ArgAsp: 1.99 ± 0.333
3.331ArgGlu: 3.331 ± 0.585
1.712ArgPhe: 1.712 ± 0.336
2.73ArgGly: 2.73 ± 0.297
0.324ArgHis: 0.324 ± 0.112
2.267ArgIle: 2.267 ± 0.285
3.655ArgLys: 3.655 ± 0.455
3.007ArgLeu: 3.007 ± 0.367
0.925ArgMet: 0.925 ± 0.244
2.175ArgAsn: 2.175 ± 0.348
0.972ArgPro: 0.972 ± 0.236
2.313ArgGln: 2.313 ± 0.352
1.296ArgArg: 1.296 ± 0.245
2.36ArgSer: 2.36 ± 0.299
2.267ArgThr: 2.267 ± 0.331
3.563ArgVal: 3.563 ± 0.479
0.278ArgTrp: 0.278 ± 0.107
1.943ArgTyr: 1.943 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
4.442SerAla: 4.442 ± 0.663
0.37SerCys: 0.37 ± 0.12
5.182SerAsp: 5.182 ± 0.475
4.442SerGlu: 4.442 ± 0.504
3.47SerPhe: 3.47 ± 0.401
5.136SerGly: 5.136 ± 0.608
0.972SerHis: 0.972 ± 0.186
3.47SerIle: 3.47 ± 0.498
5.598SerLys: 5.598 ± 0.638
6.107SerLeu: 6.107 ± 0.622
1.203SerMet: 1.203 ± 0.229
5.413SerAsn: 5.413 ± 0.447
1.712SerPro: 1.712 ± 0.279
2.637SerGln: 2.637 ± 0.37
2.313SerArg: 2.313 ± 0.414
6.015SerSer: 6.015 ± 0.792
4.025SerThr: 4.025 ± 0.499
4.719SerVal: 4.719 ± 0.454
0.879SerTrp: 0.879 ± 0.174
2.545SerTyr: 2.545 ± 0.343
0.0SerXaa: 0.0 ± 0.0
Thr
4.442ThrAla: 4.442 ± 0.83
0.648ThrCys: 0.648 ± 0.214
3.794ThrAsp: 3.794 ± 0.442
3.563ThrGlu: 3.563 ± 0.467
2.36ThrPhe: 2.36 ± 0.296
3.701ThrGly: 3.701 ± 0.651
0.925ThrHis: 0.925 ± 0.215
4.396ThrIle: 4.396 ± 0.538
5.228ThrLys: 5.228 ± 0.749
4.257ThrLeu: 4.257 ± 0.523
0.787ThrMet: 0.787 ± 0.179
3.887ThrAsn: 3.887 ± 0.517
1.666ThrPro: 1.666 ± 0.25
2.036ThrGln: 2.036 ± 0.341
2.082ThrArg: 2.082 ± 0.251
4.581ThrSer: 4.581 ± 0.58
4.349ThrThr: 4.349 ± 0.805
4.488ThrVal: 4.488 ± 0.437
0.694ThrTrp: 0.694 ± 0.24
2.73ThrTyr: 2.73 ± 0.325
0.0ThrXaa: 0.0 ± 0.0
Val
4.719ValAla: 4.719 ± 0.59
0.416ValCys: 0.416 ± 0.136
4.303ValAsp: 4.303 ± 0.489
3.979ValGlu: 3.979 ± 0.45
2.545ValPhe: 2.545 ± 0.419
4.673ValGly: 4.673 ± 0.548
0.925ValHis: 0.925 ± 0.223
4.303ValIle: 4.303 ± 0.665
5.691ValLys: 5.691 ± 0.613
4.812ValLeu: 4.812 ± 0.578
0.879ValMet: 0.879 ± 0.15
5.182ValAsn: 5.182 ± 0.564
1.434ValPro: 1.434 ± 0.263
2.175ValGln: 2.175 ± 0.46
2.128ValArg: 2.128 ± 0.314
5.367ValSer: 5.367 ± 0.533
5.043ValThr: 5.043 ± 0.577
4.581ValVal: 4.581 ± 0.551
0.972ValTrp: 0.972 ± 0.219
2.869ValTyr: 2.869 ± 0.463
0.0ValXaa: 0.0 ± 0.0
Trp
0.601TrpAla: 0.601 ± 0.135
0.416TrpCys: 0.416 ± 0.147
0.509TrpAsp: 0.509 ± 0.146
0.787TrpGlu: 0.787 ± 0.205
1.481TrpPhe: 1.481 ± 0.312
0.601TrpGly: 0.601 ± 0.197
0.093TrpHis: 0.093 ± 0.061
0.648TrpIle: 0.648 ± 0.206
1.11TrpLys: 1.11 ± 0.184
0.833TrpLeu: 0.833 ± 0.179
0.093TrpMet: 0.093 ± 0.077
0.694TrpAsn: 0.694 ± 0.183
0.046TrpPro: 0.046 ± 0.05
0.231TrpGln: 0.231 ± 0.119
0.37TrpArg: 0.37 ± 0.119
0.972TrpSer: 0.972 ± 0.197
0.74TrpThr: 0.74 ± 0.189
0.879TrpVal: 0.879 ± 0.247
0.093TrpTrp: 0.093 ± 0.055
0.694TrpTyr: 0.694 ± 0.191
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.452TyrAla: 2.452 ± 0.298
0.37TyrCys: 0.37 ± 0.122
2.684TyrAsp: 2.684 ± 0.325
2.36TyrGlu: 2.36 ± 0.279
1.943TyrPhe: 1.943 ± 0.442
3.193TyrGly: 3.193 ± 0.359
0.972TyrHis: 0.972 ± 0.275
2.082TyrIle: 2.082 ± 0.355
3.794TyrLys: 3.794 ± 0.448
3.979TyrLeu: 3.979 ± 0.713
0.787TyrMet: 0.787 ± 0.186
2.221TyrAsn: 2.221 ± 0.324
1.666TyrPro: 1.666 ± 0.34
1.804TyrGln: 1.804 ± 0.276
2.036TyrArg: 2.036 ± 0.278
2.869TyrSer: 2.869 ± 0.357
1.804TyrThr: 1.804 ± 0.305
3.47TyrVal: 3.47 ± 0.425
0.601TyrTrp: 0.601 ± 0.161
1.712TyrTyr: 1.712 ± 0.326
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (21614 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski