Vibrio phage 11895-B1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QQR2|M4QQR2_9CAUD Uncharacterized protein OS=Vibrio phage 11895-B1 OX=754075 GN=VPHG_00049 PE=4 SV=1
MM1 pKa = 7.58TNTTEE6 pKa = 4.36IKK8 pKa = 9.7YY9 pKa = 8.47TFANLNDD16 pKa = 3.73SQKK19 pKa = 10.87QKK21 pKa = 10.98LVDD24 pKa = 3.56RR25 pKa = 11.84MLNDD29 pKa = 3.27HH30 pKa = 7.11PLRR33 pKa = 11.84LQNTLVEE40 pKa = 4.29YY41 pKa = 10.44VLQKK45 pKa = 10.78SYY47 pKa = 11.5EE48 pKa = 4.14DD49 pKa = 3.51LDD51 pKa = 4.35APFSYY56 pKa = 11.17EE57 pKa = 4.36DD58 pKa = 3.18ITNFEE63 pKa = 4.87YY64 pKa = 11.14YY65 pKa = 10.95GEE67 pKa = 4.2VCINDD72 pKa = 2.96EE73 pKa = 4.09WEE75 pKa = 4.14EE76 pKa = 3.98FTEE79 pKa = 4.29SEE81 pKa = 3.96RR82 pKa = 11.84DD83 pKa = 3.5EE84 pKa = 4.42KK85 pKa = 11.44VSEE88 pKa = 4.54LEE90 pKa = 5.15DD91 pKa = 3.56EE92 pKa = 4.42QTDD95 pKa = 4.67LEE97 pKa = 5.11IDD99 pKa = 3.61DD100 pKa = 5.22PEE102 pKa = 3.92WDD104 pKa = 4.43EE105 pKa = 3.87IQEE108 pKa = 5.31IIDD111 pKa = 4.56DD112 pKa = 4.49LNNMDD117 pKa = 5.46FDD119 pKa = 4.19QLPEE123 pKa = 3.83IYY125 pKa = 9.86QWFSCSDD132 pKa = 3.01WLIRR136 pKa = 11.84EE137 pKa = 4.52LEE139 pKa = 4.35SKK141 pKa = 9.64GQCTLDD147 pKa = 3.37DD148 pKa = 4.43EE149 pKa = 4.91FWGRR153 pKa = 11.84QCCGQSVVLDD163 pKa = 3.43NVMQEE168 pKa = 3.67IAFEE172 pKa = 4.16YY173 pKa = 10.56ACDD176 pKa = 3.62YY177 pKa = 10.46GNNYY181 pKa = 7.91LTADD185 pKa = 3.53QFFTLNN191 pKa = 3.37

Molecular weight: 22.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QR01|M4QR01_9CAUD Uncharacterized protein OS=Vibrio phage 11895-B1 OX=754075 GN=VPHG_00018 PE=4 SV=1
MM1 pKa = 7.41PVIRR5 pKa = 11.84STKK8 pKa = 9.61NGKK11 pKa = 8.36PCYY14 pKa = 9.49KK15 pKa = 10.18VQNTDD20 pKa = 1.81HH21 pKa = 7.3CYY23 pKa = 9.99PYY25 pKa = 10.08TAGDD29 pKa = 3.33EE30 pKa = 4.31ASRR33 pKa = 11.84KK34 pKa = 7.06RR35 pKa = 11.84TYY37 pKa = 10.62NAAVKK42 pKa = 9.01QLRR45 pKa = 11.84AIEE48 pKa = 4.07SSKK51 pKa = 10.47YY52 pKa = 9.51RR53 pKa = 11.84RR54 pKa = 11.84KK55 pKa = 8.27TRR57 pKa = 3.16

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

196

0

196

37303

49

836

190.3

21.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.689 ± 0.241

1.823 ± 0.1

6.895 ± 0.144

7.562 ± 0.179

4.152 ± 0.092

5.865 ± 0.131

1.978 ± 0.078

6.364 ± 0.144

8.238 ± 0.249

8.168 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.093

5.608 ± 0.14

2.917 ± 0.105

3.388 ± 0.142

4.091 ± 0.125

6.654 ± 0.141

5.965 ± 0.207

6.761 ± 0.152

1.662 ± 0.079

4.686 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski