Propionibacterium australiense
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2569 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A383S3N0|A0A383S3N0_9ACTN ABC transporter OS=Propionibacterium australiense OX=119981 GN=yusV_3 PE=4 SV=1
MM1 pKa = 6.89 NTTHH5 pKa = 7.43 RR6 pKa = 11.84 GLTRR10 pKa = 11.84 RR11 pKa = 11.84 FAAFAGLGAVGAMMLTACSGDD32 pKa = 3.86 DD33 pKa = 3.65 EE34 pKa = 4.8 LVDD37 pKa = 3.61 MGIPMSDD44 pKa = 2.79 GTYY47 pKa = 9.41 TGQSNEE53 pKa = 4.26 DD54 pKa = 3.56 DD55 pKa = 3.62 QGSVGTVTITVEE67 pKa = 3.67 GGRR70 pKa = 11.84 IIEE73 pKa = 4.17 ASYY76 pKa = 8.86 EE77 pKa = 4.3 TVEE80 pKa = 4.51 SDD82 pKa = 3.89 GTVKK86 pKa = 10.86 DD87 pKa = 3.67 EE88 pKa = 4.73 NYY90 pKa = 10.77 GKK92 pKa = 10.35 DD93 pKa = 3.32 SSGEE97 pKa = 3.84 IANAEE102 pKa = 4.21 YY103 pKa = 10.4 YY104 pKa = 10.38 QRR106 pKa = 11.84 AQAAVASYY114 pKa = 8.98 EE115 pKa = 4.15 QYY117 pKa = 10.97 SAALVEE123 pKa = 4.62 AQDD126 pKa = 3.71 PTAVDD131 pKa = 4.44 TISGATVAHH140 pKa = 5.74 SQFLQAALRR149 pKa = 11.84 AVYY152 pKa = 9.36 QAQGVEE158 pKa = 3.98 DD159 pKa = 4.93 DD160 pKa = 4.59 GRR162 pKa = 11.84 ADD164 pKa = 4.13 TINMPGLGLEE174 pKa = 4.59 DD175 pKa = 3.5 EE176 pKa = 5.63 DD177 pKa = 4.62 YY178 pKa = 11.72
Molecular weight: 18.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.668
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.579
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 1.888
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A383S5E2|A0A383S5E2_9ACTN Fer4_NifH OS=Propionibacterium australiense OX=119981 GN=NCTC13651_00514 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.55 LLKK22 pKa = 8.44 KK23 pKa = 9.1 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGKK33 pKa = 9.79
Molecular weight: 4.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2569
0
2569
845120
21
2591
329.0
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.553 ± 0.064
0.976 ± 0.014
6.099 ± 0.041
5.752 ± 0.042
2.842 ± 0.029
9.144 ± 0.045
2.106 ± 0.023
4.468 ± 0.038
2.054 ± 0.039
9.921 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.261 ± 0.023
2.186 ± 0.027
5.376 ± 0.031
3.156 ± 0.032
7.465 ± 0.053
5.63 ± 0.036
6.024 ± 0.043
8.4 ± 0.045
1.502 ± 0.022
2.075 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here