Aeropyrum coil-shaped virus
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7Q331|J7Q331_9VIRU Uncharacterized protein OS=Aeropyrum coil-shaped virus OX=1157339 GN=50-51 PE=4 SV=1
MM1 pKa = 7.48 PTFKK5 pKa = 10.81 VYY7 pKa = 10.16 LWEE10 pKa = 4.14 WDD12 pKa = 3.9 TEE14 pKa = 4.15 NGEE17 pKa = 4.24 YY18 pKa = 10.55 VYY20 pKa = 10.79 RR21 pKa = 11.84 DD22 pKa = 3.62 YY23 pKa = 11.56 EE24 pKa = 4.52 GEE26 pKa = 4.15 TVEE29 pKa = 5.02 AANEE33 pKa = 3.7 EE34 pKa = 4.48 DD35 pKa = 3.18 LWRR38 pKa = 11.84 TLEE41 pKa = 3.73 SWLRR45 pKa = 11.84 DD46 pKa = 3.43 IAKK49 pKa = 9.77 EE50 pKa = 3.75 AAGEE54 pKa = 4.15 GWDD57 pKa = 5.43 CEE59 pKa = 4.24 FTPNAEE65 pKa = 4.32 LLCTRR70 pKa = 11.84 CEE72 pKa = 3.87 EE73 pKa = 5.21 GYY75 pKa = 10.61 CEE77 pKa = 4.19 SRR79 pKa = 11.84 QIGYY83 pKa = 9.6 DD84 pKa = 3.53 VEE86 pKa = 4.58 KK87 pKa = 11.0 VSPP90 pKa = 3.73
Molecular weight: 10.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.848
IPC2_protein 4.101
IPC_protein 3.986
Toseland 3.834
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.783
Rodwell 3.821
Grimsley 3.745
Solomon 3.923
Lehninger 3.872
Nozaki 4.05
DTASelect 4.139
Thurlkill 3.846
EMBOSS 3.808
Sillero 4.088
Patrickios 0.947
IPC_peptide 3.923
IPC2_peptide 4.088
IPC2.peptide.svr19 3.998
Protein with the highest isoelectric point:
>tr|J7Q7K5|J7Q7K5_9VIRU Uncharacterized protein OS=Aeropyrum coil-shaped virus OX=1157339 GN=49-111 PE=4 SV=1
MM1 pKa = 6.99 VARR4 pKa = 11.84 VMVRR8 pKa = 11.84 VMASPLDD15 pKa = 3.68 LSSIFGQLVPIFVFIMIFVLLITLFKK41 pKa = 10.45 TLFRR45 pKa = 11.84 TISGASARR53 pKa = 11.84 RR54 pKa = 11.84 FPSILARR61 pKa = 11.84 IRR63 pKa = 11.84 GRR65 pKa = 3.37
Molecular weight: 7.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 10.716
IPC_protein 12.31
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.471
Wikipedia 12.954
Rodwell 12.047
Grimsley 12.515
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.471
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.798
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.171
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
7751
37
407
136.0
15.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.418 ± 0.383
0.929 ± 0.166
4.619 ± 0.266
8.67 ± 0.491
3.845 ± 0.235
6.335 ± 0.337
1.419 ± 0.139
7.405 ± 0.281
6.09 ± 0.462
9.663 ± 0.431
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.161 ± 0.299
2.838 ± 0.219
4.038 ± 0.274
2.322 ± 0.18
7.651 ± 0.36
5.083 ± 0.282
4.193 ± 0.343
8.902 ± 0.373
1.445 ± 0.146
3.974 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here