Prevotella sp. oral taxon 299 str. F0039
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1947 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3IDD1|D3IDD1_9BACT 3-deoxy-D-manno-octulosonic acid transferase OS=Prevotella sp. oral taxon 299 str. F0039 OX=575614 GN=HMPREF0669_01435 PE=3 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.11 LASKK10 pKa = 11.0 LEE12 pKa = 4.24 DD13 pKa = 4.06 APWPATKK20 pKa = 10.56 EE21 pKa = 3.91 EE22 pKa = 4.15 LLDD25 pKa = 3.56 YY26 pKa = 10.55 ASRR29 pKa = 11.84 SGAPLEE35 pKa = 4.32 VIEE38 pKa = 4.32 NLEE41 pKa = 4.25 EE42 pKa = 5.14 IEE44 pKa = 5.12 DD45 pKa = 3.82 EE46 pKa = 4.4 GDD48 pKa = 3.48 VYY50 pKa = 11.37 EE51 pKa = 4.97 SIEE54 pKa = 5.06 DD55 pKa = 3.26 IWPDD59 pKa = 3.64 YY60 pKa = 10.2 PSKK63 pKa = 11.13 EE64 pKa = 3.65 DD65 pKa = 3.48 FLFNEE70 pKa = 4.91 DD71 pKa = 3.49 EE72 pKa = 4.45 YY73 pKa = 12.0
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.863
IPC2_protein 3.605
IPC_protein 3.516
Toseland 3.35
ProMoST 3.681
Dawson 3.503
Bjellqvist 3.668
Wikipedia 3.414
Rodwell 3.363
Grimsley 3.261
Solomon 3.465
Lehninger 3.427
Nozaki 3.63
DTASelect 3.745
Thurlkill 3.401
EMBOSS 3.427
Sillero 3.643
Patrickios 1.761
IPC_peptide 3.465
IPC2_peptide 3.617
IPC2.peptide.svr19 3.66
Protein with the highest isoelectric point:
>tr|T1TG62|T1TG62_9BACT Tyr recombinase domain-containing protein OS=Prevotella sp. oral taxon 299 str. F0039 OX=575614 GN=HMPREF0669_01980 PE=4 SV=1
MM1 pKa = 7.85 PNGKK5 pKa = 9.19 KK6 pKa = 10.25 KK7 pKa = 10.12 KK8 pKa = 7.0 GHH10 pKa = 6.14 KK11 pKa = 9.06 MATHH15 pKa = 6.13 KK16 pKa = 10.39 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.25 NRR25 pKa = 11.84 HH26 pKa = 4.69 KK27 pKa = 11.1 SKK29 pKa = 11.1
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.862
IPC_protein 12.325
Toseland 12.53
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.647
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.013
Sillero 12.53
Patrickios 12.369
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1947
0
1947
703941
29
2509
361.6
40.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.679 ± 0.052
1.152 ± 0.016
5.25 ± 0.04
6.274 ± 0.056
4.675 ± 0.038
6.307 ± 0.051
1.919 ± 0.023
7.372 ± 0.062
7.392 ± 0.048
9.28 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.03
5.902 ± 0.053
3.44 ± 0.026
3.723 ± 0.029
4.174 ± 0.035
6.392 ± 0.042
5.844 ± 0.043
6.253 ± 0.043
1.138 ± 0.02
4.283 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here