Fusarium oxysporum f. sp. cubense (strain race 4) (Panama disease fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium oxysporum species complex

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N1R907|N1R907_FUSC4 Histidine biosynthesis trifunctional protein OS=Fusarium oxysporum f. sp. cubense (strain race 4) OX=2502994 GN=FOC4_g10014644 PE=3 SV=1
MM1 pKa = 7.78KK2 pKa = 10.45SQILLTTAMGASGVLSQAGHH22 pKa = 6.32GAMSLDD28 pKa = 3.91TWCVTYY34 pKa = 10.81LSTYY38 pKa = 9.42LVPVANPDD46 pKa = 3.06QATDD50 pKa = 3.56VSSSQPPFTGNSSVPTDD67 pKa = 3.51CVTEE71 pKa = 4.3TEE73 pKa = 4.4SQSALTSDD81 pKa = 3.38TSVFTSVAVSSSVEE95 pKa = 4.1TEE97 pKa = 3.74LTEE100 pKa = 4.22AQSGSEE106 pKa = 4.05TVSFDD111 pKa = 3.32STAGTLADD119 pKa = 4.2QITSASSEE127 pKa = 4.32STISTDD133 pKa = 3.73ALTTSTSIIEE143 pKa = 4.01PPGRR147 pKa = 11.84SVIFLISAPNTRR159 pKa = 11.84KK160 pKa = 9.85RR161 pKa = 11.84QNTDD165 pKa = 2.14KK166 pKa = 11.42DD167 pKa = 3.86GVLFFYY173 pKa = 10.82DD174 pKa = 3.44GEE176 pKa = 4.46DD177 pKa = 3.51YY178 pKa = 11.15KK179 pKa = 11.12EE180 pKa = 4.24LVMGPTPPAGAVTRR194 pKa = 11.84LFTTAGRR201 pKa = 11.84SLQVDD206 pKa = 4.55LPGGEE211 pKa = 4.95AGFCQTSDD219 pKa = 2.56GRR221 pKa = 11.84VYY223 pKa = 10.12VTFTRR228 pKa = 11.84GPAGCEE234 pKa = 3.52AVSLDD239 pKa = 3.93VYY241 pKa = 10.62DD242 pKa = 5.61EE243 pKa = 4.32RR244 pKa = 11.84QCQNGRR250 pKa = 11.84LVGIDD255 pKa = 3.34TTTSAMKK262 pKa = 9.12TATSEE267 pKa = 4.38VISSGNATSAEE278 pKa = 4.06GSATTEE284 pKa = 4.07TTEE287 pKa = 4.21TTSEE291 pKa = 4.31SIATSSNVAQTQSVGPVSQTTHH313 pKa = 6.7SEE315 pKa = 3.72ASTAASSRR323 pKa = 11.84AVEE326 pKa = 4.22SSTSVLSTTTTTTVVSDD343 pKa = 5.12ASTQSQASSSVLGEE357 pKa = 4.08STTDD361 pKa = 3.18EE362 pKa = 4.47VFPTTAPSLVSTSAQAEE379 pKa = 4.2EE380 pKa = 4.34STSIKK385 pKa = 10.11PSSQASSEE393 pKa = 4.26TTEE396 pKa = 4.02SGTTSSEE403 pKa = 3.74EE404 pKa = 4.11STSDD408 pKa = 3.23VALEE412 pKa = 4.21TTTEE416 pKa = 3.83ARR418 pKa = 11.84TSTRR422 pKa = 11.84EE423 pKa = 3.48TTAADD428 pKa = 3.49EE429 pKa = 4.35TSTEE433 pKa = 4.1NEE435 pKa = 4.31TTTEE439 pKa = 3.84AATEE443 pKa = 4.33SEE445 pKa = 4.48TTTTEE450 pKa = 3.56SSAPSEE456 pKa = 5.09DD457 pKa = 3.31ISTTDD462 pKa = 2.98VTAVDD467 pKa = 3.81SSTAATTTTAALACTDD483 pKa = 3.98LSDD486 pKa = 4.9PYY488 pKa = 10.16MDD490 pKa = 3.89STGTTYY496 pKa = 11.64ALLCNTDD503 pKa = 3.15VNGYY507 pKa = 7.22TSINGFTAASFVACIEE523 pKa = 4.06ACSVYY528 pKa = 10.52VGCAGIEE535 pKa = 4.07FLKK538 pKa = 10.06STGYY542 pKa = 8.42CTLFNSSTGSSPTTDD557 pKa = 2.96YY558 pKa = 11.42DD559 pKa = 3.35IALGALRR566 pKa = 11.84EE567 pKa = 4.33GG568 pKa = 3.89

Molecular weight: 58.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N1RB97|N1RB97_FUSC4 HET domain-containing protein (Fragment) OS=Fusarium oxysporum f. sp. cubense (strain race 4) OX=2502994 GN=FOC4_g10006368 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APAHH26 pKa = 4.84SHH28 pKa = 5.33RR29 pKa = 11.84HH30 pKa = 4.03TTTTTTTTMKK40 pKa = 9.74PRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84GMFGGGAGRR53 pKa = 11.84RR54 pKa = 11.84THH56 pKa = 6.46ATTTAPVHH64 pKa = 4.87HH65 pKa = 6.63HH66 pKa = 5.14QRR68 pKa = 11.84RR69 pKa = 11.84PSMKK73 pKa = 10.02DD74 pKa = 2.95KK75 pKa = 11.41VSGALLKK82 pKa = 11.04LKK84 pKa = 10.68GSLTRR89 pKa = 11.84RR90 pKa = 11.84PGVKK94 pKa = 9.89AAGTRR99 pKa = 11.84RR100 pKa = 11.84MRR102 pKa = 11.84GTDD105 pKa = 3.02GRR107 pKa = 11.84GARR110 pKa = 11.84HH111 pKa = 5.87HH112 pKa = 7.23RR113 pKa = 11.84YY114 pKa = 9.44

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14157

0

14157

6288100

26

6475

444.2

49.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.354 ± 0.019

1.319 ± 0.009

5.8 ± 0.016

6.151 ± 0.021

3.845 ± 0.013

6.812 ± 0.02

2.368 ± 0.01

5.179 ± 0.015

5.038 ± 0.018

8.865 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.244 ± 0.008

3.776 ± 0.01

5.78 ± 0.022

3.947 ± 0.014

5.781 ± 0.017

7.962 ± 0.021

5.962 ± 0.019

6.113 ± 0.013

1.587 ± 0.008

2.842 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski