Lactobacillus tucceti DSM 20183
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2050 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1J1X3|A0A0R1J1X3_9LACO Iron(III)-compound ABC transporter permease OS=Lactobacillus tucceti DSM 20183 OX=1423811 GN=FC72_GL001387 PE=3 SV=1
MM1 pKa = 7.17 TKK3 pKa = 10.51 VKK5 pKa = 9.98 IVYY8 pKa = 10.36 ASITGNDD15 pKa = 2.9 EE16 pKa = 4.44 DD17 pKa = 3.41 IAYY20 pKa = 10.67 VLTEE24 pKa = 3.89 KK25 pKa = 11.08 FEE27 pKa = 4.84 DD28 pKa = 3.49 LGADD32 pKa = 3.44 VEE34 pKa = 4.36 MSEE37 pKa = 5.47 ISQTDD42 pKa = 3.07 AADD45 pKa = 4.2 FEE47 pKa = 4.94 DD48 pKa = 5.84 ADD50 pKa = 3.65 ICVIASYY57 pKa = 9.52 TYY59 pKa = 10.7 DD60 pKa = 3.21 QGIVPDD66 pKa = 3.95 EE67 pKa = 4.32 ALDD70 pKa = 4.11 FYY72 pKa = 11.53 EE73 pKa = 6.13 DD74 pKa = 3.86 MQDD77 pKa = 3.45 LDD79 pKa = 5.06 LDD81 pKa = 3.94 GKK83 pKa = 11.41 VYY85 pKa = 8.16 GTCGSGDD92 pKa = 3.64 TFYY95 pKa = 11.36 EE96 pKa = 4.78 DD97 pKa = 3.17 YY98 pKa = 10.94 CRR100 pKa = 11.84 AVDD103 pKa = 3.56 EE104 pKa = 4.6 FAAIFEE110 pKa = 4.52 QVGATKK116 pKa = 10.08 GAEE119 pKa = 3.95 PVRR122 pKa = 11.84 VEE124 pKa = 4.85 LEE126 pKa = 4.12 PEE128 pKa = 4.02 QEE130 pKa = 5.65 DD131 pKa = 4.49 IDD133 pKa = 4.4 HH134 pKa = 7.3 LDD136 pKa = 3.4 NFAEE140 pKa = 4.16 EE141 pKa = 4.48 LVGKK145 pKa = 10.06 AKK147 pKa = 10.61 EE148 pKa = 3.94 MDD150 pKa = 3.44 HH151 pKa = 7.0
Molecular weight: 16.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.656
IPC_protein 3.656
Toseland 3.439
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.567
Rodwell 3.478
Grimsley 3.35
Solomon 3.63
Lehninger 3.592
Nozaki 3.757
DTASelect 3.973
Thurlkill 3.49
EMBOSS 3.579
Sillero 3.77
Patrickios 1.062
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A0R1IZ81|A0A0R1IZ81_9LACO Uncharacterized protein OS=Lactobacillus tucceti DSM 20183 OX=1423811 GN=FC72_GL002029 PE=4 SV=1
MM1 pKa = 5.72 TTKK4 pKa = 9.85 RR5 pKa = 11.84 TFQPKK10 pKa = 8.33 KK11 pKa = 7.5 RR12 pKa = 11.84 HH13 pKa = 5.56 RR14 pKa = 11.84 EE15 pKa = 3.79 RR16 pKa = 11.84 VHH18 pKa = 7.7 GFMKK22 pKa = 10.54 RR23 pKa = 11.84 MSTSNGRR30 pKa = 11.84 KK31 pKa = 8.4 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.2 KK39 pKa = 10.05 GRR41 pKa = 11.84 KK42 pKa = 8.66 VLSAA46 pKa = 4.05
Molecular weight: 5.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2050
0
2050
631509
46
2417
308.1
34.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.436 ± 0.061
0.457 ± 0.012
6.443 ± 0.058
5.693 ± 0.064
4.453 ± 0.045
6.585 ± 0.051
1.752 ± 0.025
8.188 ± 0.056
7.668 ± 0.053
9.294 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.729 ± 0.028
5.901 ± 0.049
3.326 ± 0.029
3.505 ± 0.035
3.332 ± 0.042
6.812 ± 0.055
5.898 ± 0.057
6.879 ± 0.047
0.915 ± 0.019
3.729 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here