Corynebacterium cystitidis DSM 20524
Average proteome isoelectric point is 5.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2787 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H9VG85|A0A1H9VG85_9CORY Long-chain acyl-CoA synthetase OS=Corynebacterium cystitidis DSM 20524 OX=1121357 GN=SAMN05661109_02227 PE=4 SV=1
MM1 pKa = 7.72 SDD3 pKa = 3.64 DD4 pKa = 3.89 TSCDD8 pKa = 2.97 GLQFNRR14 pKa = 11.84 TPVYY18 pKa = 9.83 ISLAIAIVLVVAVLFGAKK36 pKa = 8.62 WYY38 pKa = 10.42 FDD40 pKa = 3.55 QATNTPVAMGAVDD53 pKa = 4.5 APEE56 pKa = 4.72 ADD58 pKa = 3.76 SPQCQSLIDD67 pKa = 4.44 DD68 pKa = 5.14 LPDD71 pKa = 3.35 EE72 pKa = 4.8 LLGHH76 pKa = 6.93 DD77 pKa = 3.74 RR78 pKa = 11.84 AEE80 pKa = 4.14 LTEE83 pKa = 4.33 PKK85 pKa = 9.9 PAGAAAWQTTEE96 pKa = 3.85 IEE98 pKa = 4.8 RR99 pKa = 11.84 ITLRR103 pKa = 11.84 CGVAVPLQYY112 pKa = 10.83 DD113 pKa = 4.39 EE114 pKa = 4.53 YY115 pKa = 11.37 AQTEE119 pKa = 4.32 QINGVEE125 pKa = 4.22 WLRR128 pKa = 11.84 VDD130 pKa = 4.97 DD131 pKa = 4.18 YY132 pKa = 11.28 TPEE135 pKa = 4.24 STLSTWYY142 pKa = 9.29 TVDD145 pKa = 3.59 RR146 pKa = 11.84 YY147 pKa = 10.23 PVVAVTADD155 pKa = 3.36 DD156 pKa = 4.02 HH157 pKa = 7.24 ALGGAEE163 pKa = 3.83 NPVEE167 pKa = 4.05 NVPVGQLEE175 pKa = 4.02 QRR177 pKa = 11.84 EE178 pKa = 4.41 VEE180 pKa = 4.73 PYY182 pKa = 8.92 PAPLSNLAAGDD193 pKa = 3.68 ASVAGACATLMEE205 pKa = 4.82 TLPEE209 pKa = 4.3 DD210 pKa = 3.69 VADD213 pKa = 5.0 GYY215 pKa = 11.7 SPLAANTGGAEE226 pKa = 4.38 LDD228 pKa = 3.69 PDD230 pKa = 4.19 TAVWTAPGAEE240 pKa = 4.32 SIVLRR245 pKa = 11.84 CGVADD250 pKa = 3.91 PEE252 pKa = 4.26 NYY254 pKa = 9.58 QPGEE258 pKa = 3.89 QLHH261 pKa = 6.06 QINDD265 pKa = 3.64 VTWFEE270 pKa = 4.16 DD271 pKa = 3.48 TQLINGSTASTWFALGRR288 pKa = 11.84 EE289 pKa = 4.41 ANVAVSMPQFVGNSAIVRR307 pKa = 11.84 ITEE310 pKa = 4.75 AIEE313 pKa = 3.86 KK314 pKa = 10.07 ALPAEE319 pKa = 4.32
Molecular weight: 34.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.91
Patrickios 1.202
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A1H9UMT5|A0A1H9UMT5_9CORY Non-specific serine/threonine protein kinase OS=Corynebacterium cystitidis DSM 20524 OX=1121357 GN=SAMN05661109_01882 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 KK16 pKa = 8.53 HH17 pKa = 4.82 GFRR20 pKa = 11.84 TRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIVSARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.64 KK38 pKa = 10.52 GRR40 pKa = 11.84 AKK42 pKa = 9.67 LTAA45 pKa = 4.21
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2787
0
2787
873279
19
3087
313.3
34.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.083 ± 0.055
0.655 ± 0.011
6.262 ± 0.042
6.262 ± 0.046
3.357 ± 0.031
8.207 ± 0.043
2.237 ± 0.021
5.162 ± 0.035
3.182 ± 0.036
9.375 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.254 ± 0.02
3.044 ± 0.025
5.008 ± 0.034
3.492 ± 0.024
6.019 ± 0.04
5.88 ± 0.037
6.367 ± 0.034
8.332 ± 0.041
1.454 ± 0.02
2.368 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here