Flavobacterium album
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3477 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1R006|A0A2S1R006_9FLAO Uncharacterized protein OS=Flavobacterium album OX=2175091 GN=HYN59_12985 PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.35 KK3 pKa = 9.89 QLLLGAFLMASMFTAKK19 pKa = 10.44 AQLSEE24 pKa = 4.47 DD25 pKa = 3.9 FEE27 pKa = 4.71 GTAFPPEE34 pKa = 3.99 GWTVEE39 pKa = 4.25 TTNPDD44 pKa = 3.39 ATWEE48 pKa = 4.07 RR49 pKa = 11.84 FTGNNSIGGTGSAGVGYY66 pKa = 10.48 DD67 pKa = 3.65 FDD69 pKa = 4.18 QDD71 pKa = 3.28 EE72 pKa = 4.68 SLISPSFNLAGANPVLTFNILMGYY96 pKa = 6.42 TWGVNPNNNYY106 pKa = 10.48 DD107 pKa = 3.94 VIVSVSNDD115 pKa = 3.11 GGTSWDD121 pKa = 3.8 QVWDD125 pKa = 4.0 EE126 pKa = 4.63 SEE128 pKa = 4.87 LGVFTDD134 pKa = 3.73 YY135 pKa = 11.32 DD136 pKa = 4.25 IIPVTVQLAAYY147 pKa = 9.48 SGDD150 pKa = 3.75 DD151 pKa = 3.11 IKK153 pKa = 11.22 IKK155 pKa = 10.67 FEE157 pKa = 4.19 YY158 pKa = 10.13 VGNDD162 pKa = 3.05 GDD164 pKa = 4.34 VLIIDD169 pKa = 5.58 DD170 pKa = 4.16 IAVVACAVPSGFSYY184 pKa = 11.21 LDD186 pKa = 3.64 PAPSLTAVNIGWDD199 pKa = 3.6 APAGSPEE206 pKa = 4.49 GYY208 pKa = 9.6 QFEE211 pKa = 4.47 YY212 pKa = 10.68 GPRR215 pKa = 11.84 GFTQGGAGSMIINPTLPAANLTDD238 pKa = 5.2 LDD240 pKa = 4.37 PSTIYY245 pKa = 10.79 DD246 pKa = 4.0 FYY248 pKa = 11.85 VRR250 pKa = 11.84 THH252 pKa = 6.78 CGGDD256 pKa = 4.56 DD257 pKa = 3.46 YY258 pKa = 11.7 SEE260 pKa = 4.45 WIGPVSFATLFDD272 pKa = 4.14 AVTPPYY278 pKa = 9.24 NTSFEE283 pKa = 4.44 EE284 pKa = 4.08 NNLDD288 pKa = 3.56 FVGWIDD294 pKa = 4.24 GTDD297 pKa = 3.34 PDD299 pKa = 5.2 AGVEE303 pKa = 3.94 PWGVNVAVEE312 pKa = 4.36 GDD314 pKa = 3.44 ATVQDD319 pKa = 4.27 GANSLFTFAPTNLDD333 pKa = 3.35 SNAWLLSRR341 pKa = 11.84 GINLTGGSQVTVSFWARR358 pKa = 11.84 NLLGQGATGSASYY371 pKa = 11.32 NLTVGNAQNAAAQTTNIATEE391 pKa = 4.2 NDD393 pKa = 3.37 LSSTTFVQKK402 pKa = 9.79 TYY404 pKa = 9.43 TFSPATSGVYY414 pKa = 9.56 YY415 pKa = 10.36 FGINNISPSNPNAQVLLVDD434 pKa = 3.96 NFSVTEE440 pKa = 3.79 ILGTKK445 pKa = 10.03 DD446 pKa = 3.13 VLASQLSIFPNPATNVINITNADD469 pKa = 3.99 NILVNGVVIADD480 pKa = 3.66 LNGRR484 pKa = 11.84 TVKK487 pKa = 10.21 SAKK490 pKa = 9.71 FDD492 pKa = 3.81 GVAEE496 pKa = 4.1 AQVNISDD503 pKa = 4.77 LANGMYY509 pKa = 9.81 MMTVSSDD516 pKa = 2.78 KK517 pKa = 10.85 GTMTKK522 pKa = 10.28 KK523 pKa = 9.91 IVKK526 pKa = 9.42 NN527 pKa = 3.66
Molecular weight: 56.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.113
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.859
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A2S1QYK8|A0A2S1QYK8_9FLAO Lipase OS=Flavobacterium album OX=2175091 GN=HYN59_10350 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.53 HH17 pKa = 4.24 GFMEE21 pKa = 4.81 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 HH41 pKa = 5.41 KK42 pKa = 10.14 LTVSSEE48 pKa = 3.92 PRR50 pKa = 11.84 HH51 pKa = 5.77 KK52 pKa = 10.61 KK53 pKa = 9.84
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3477
0
3477
1167212
26
4638
335.7
37.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.572 ± 0.057
0.809 ± 0.016
5.621 ± 0.033
6.199 ± 0.059
4.981 ± 0.036
6.987 ± 0.052
1.747 ± 0.021
7.216 ± 0.047
6.991 ± 0.07
8.773 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.408 ± 0.024
5.685 ± 0.042
3.766 ± 0.031
3.286 ± 0.024
3.637 ± 0.038
6.16 ± 0.043
6.382 ± 0.096
6.464 ± 0.036
1.061 ± 0.013
4.256 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here