Amino acid dipepetide frequency for Flavobacterium album

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.643AlaAla: 6.643 ± 0.127
0.653AlaCys: 0.653 ± 0.027
4.174AlaAsp: 4.174 ± 0.062
4.613AlaGlu: 4.613 ± 0.075
3.704AlaPhe: 3.704 ± 0.067
5.774AlaGly: 5.774 ± 0.113
1.133AlaHis: 1.133 ± 0.033
5.488AlaIle: 5.488 ± 0.085
4.853AlaLys: 4.853 ± 0.089
6.907AlaLeu: 6.907 ± 0.082
1.819AlaMet: 1.819 ± 0.044
3.934AlaAsn: 3.934 ± 0.071
2.553AlaPro: 2.553 ± 0.072
2.769AlaGln: 2.769 ± 0.054
2.354AlaArg: 2.354 ± 0.055
4.597AlaSer: 4.597 ± 0.077
4.929AlaThr: 4.929 ± 0.162
5.191AlaVal: 5.191 ± 0.074
0.737AlaTrp: 0.737 ± 0.023
2.898AlaTyr: 2.898 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.557CysAla: 0.557 ± 0.035
0.117CysCys: 0.117 ± 0.011
0.475CysAsp: 0.475 ± 0.023
0.469CysGlu: 0.469 ± 0.023
0.394CysPhe: 0.394 ± 0.02
0.737CysGly: 0.737 ± 0.034
0.191CysHis: 0.191 ± 0.013
0.614CysIle: 0.614 ± 0.025
0.404CysLys: 0.404 ± 0.018
0.671CysLeu: 0.671 ± 0.026
0.152CysMet: 0.152 ± 0.01
0.425CysAsn: 0.425 ± 0.022
0.332CysPro: 0.332 ± 0.023
0.2CysGln: 0.2 ± 0.014
0.285CysArg: 0.285 ± 0.016
0.636CysSer: 0.636 ± 0.031
0.583CysThr: 0.583 ± 0.041
0.446CysVal: 0.446 ± 0.027
0.077CysTrp: 0.077 ± 0.008
0.324CysTyr: 0.324 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.276AspAla: 4.276 ± 0.062
0.438AspCys: 0.438 ± 0.026
2.963AspAsp: 2.963 ± 0.066
3.677AspGlu: 3.677 ± 0.061
3.335AspPhe: 3.335 ± 0.055
4.266AspGly: 4.266 ± 0.078
0.888AspHis: 0.888 ± 0.029
4.404AspIle: 4.404 ± 0.075
4.064AspLys: 4.064 ± 0.07
4.554AspLeu: 4.554 ± 0.071
1.331AspMet: 1.331 ± 0.034
3.33AspAsn: 3.33 ± 0.063
2.006AspPro: 2.006 ± 0.046
1.234AspGln: 1.234 ± 0.032
2.118AspArg: 2.118 ± 0.043
2.985AspSer: 2.985 ± 0.058
3.074AspThr: 3.074 ± 0.055
3.678AspVal: 3.678 ± 0.055
0.73AspTrp: 0.73 ± 0.024
2.857AspTyr: 2.857 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
4.749GluAla: 4.749 ± 0.072
0.38GluCys: 0.38 ± 0.02
3.302GluAsp: 3.302 ± 0.057
4.41GluGlu: 4.41 ± 0.084
2.766GluPhe: 2.766 ± 0.053
4.064GluGly: 4.064 ± 0.065
1.11GluHis: 1.11 ± 0.03
4.73GluIle: 4.73 ± 0.079
5.554GluLys: 5.554 ± 0.09
5.549GluLeu: 5.549 ± 0.089
1.661GluMet: 1.661 ± 0.041
3.802GluAsn: 3.802 ± 0.062
1.767GluPro: 1.767 ± 0.041
2.186GluGln: 2.186 ± 0.051
2.485GluArg: 2.485 ± 0.049
2.988GluSer: 2.988 ± 0.058
3.447GluThr: 3.447 ± 0.054
3.963GluVal: 3.963 ± 0.063
0.633GluTrp: 0.633 ± 0.024
2.447GluTyr: 2.447 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
3.464PheAla: 3.464 ± 0.062
0.482PheCys: 0.482 ± 0.021
3.262PheAsp: 3.262 ± 0.06
3.173PheGlu: 3.173 ± 0.059
2.519PhePhe: 2.519 ± 0.059
3.546PheGly: 3.546 ± 0.053
0.828PheHis: 0.828 ± 0.025
3.473PheIle: 3.473 ± 0.067
2.911PheLys: 2.911 ± 0.06
4.175PheLeu: 4.175 ± 0.082
1.169PheMet: 1.169 ± 0.032
2.887PheAsn: 2.887 ± 0.061
1.713PhePro: 1.713 ± 0.036
1.249PheGln: 1.249 ± 0.036
2.077PheArg: 2.077 ± 0.05
3.717PheSer: 3.717 ± 0.068
3.657PheThr: 3.657 ± 0.07
2.853PheVal: 2.853 ± 0.055
0.535PheTrp: 0.535 ± 0.02
2.119PheTyr: 2.119 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
4.74GlyAla: 4.74 ± 0.087
0.802GlyCys: 0.802 ± 0.047
3.506GlyAsp: 3.506 ± 0.058
3.769GlyGlu: 3.769 ± 0.063
3.61GlyPhe: 3.61 ± 0.059
5.076GlyGly: 5.076 ± 0.107
1.261GlyHis: 1.261 ± 0.038
5.379GlyIle: 5.379 ± 0.071
5.06GlyLys: 5.06 ± 0.09
5.865GlyLeu: 5.865 ± 0.08
1.841GlyMet: 1.841 ± 0.045
4.075GlyAsn: 4.075 ± 0.08
1.624GlyPro: 1.624 ± 0.055
2.319GlyGln: 2.319 ± 0.047
2.494GlyArg: 2.494 ± 0.056
4.645GlySer: 4.645 ± 0.083
5.507GlyThr: 5.507 ± 0.194
4.273GlyVal: 4.273 ± 0.073
0.86GlyTrp: 0.86 ± 0.034
3.16GlyTyr: 3.16 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
1.062HisAla: 1.062 ± 0.031
0.164HisCys: 0.164 ± 0.013
0.985HisAsp: 0.985 ± 0.033
1.06HisGlu: 1.06 ± 0.031
1.08HisPhe: 1.08 ± 0.031
1.115HisGly: 1.115 ± 0.036
0.446HisHis: 0.446 ± 0.025
1.343HisIle: 1.343 ± 0.037
1.098HisLys: 1.098 ± 0.031
1.612HisLeu: 1.612 ± 0.039
0.34HisMet: 0.34 ± 0.017
1.014HisAsn: 1.014 ± 0.033
0.852HisPro: 0.852 ± 0.029
0.533HisGln: 0.533 ± 0.023
0.695HisArg: 0.695 ± 0.024
1.057HisSer: 1.057 ± 0.032
1.019HisThr: 1.019 ± 0.034
0.919HisVal: 0.919 ± 0.028
0.209HisTrp: 0.209 ± 0.016
0.861HisTyr: 0.861 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.982IleAla: 5.982 ± 0.077
0.603IleCys: 0.603 ± 0.027
4.394IleAsp: 4.394 ± 0.068
4.5IleGlu: 4.5 ± 0.082
3.203IlePhe: 3.203 ± 0.062
4.612IleGly: 4.612 ± 0.071
1.184IleHis: 1.184 ± 0.033
5.243IleIle: 5.243 ± 0.079
4.854IleLys: 4.854 ± 0.086
5.834IleLeu: 5.834 ± 0.083
1.537IleMet: 1.537 ± 0.037
4.133IleAsn: 4.133 ± 0.073
3.002IlePro: 3.002 ± 0.051
2.123IleGln: 2.123 ± 0.046
2.67IleArg: 2.67 ± 0.057
4.841IleSer: 4.841 ± 0.069
5.267IleThr: 5.267 ± 0.099
4.692IleVal: 4.692 ± 0.065
0.654IleTrp: 0.654 ± 0.024
2.836IleTyr: 2.836 ± 0.059
0.0IleXaa: 0.0 ± 0.0
Lys
5.135LysAla: 5.135 ± 0.089
0.331LysCys: 0.331 ± 0.018
4.342LysAsp: 4.342 ± 0.079
5.399LysGlu: 5.399 ± 0.102
2.783LysPhe: 2.783 ± 0.056
4.475LysGly: 4.475 ± 0.088
1.18LysHis: 1.18 ± 0.033
5.166LysIle: 5.166 ± 0.074
6.169LysLys: 6.169 ± 0.104
5.786LysLeu: 5.786 ± 0.095
2.101LysMet: 2.101 ± 0.04
4.1LysAsn: 4.1 ± 0.073
2.504LysPro: 2.504 ± 0.056
2.399LysGln: 2.399 ± 0.052
2.503LysArg: 2.503 ± 0.05
3.618LysSer: 3.618 ± 0.069
3.998LysThr: 3.998 ± 0.07
4.316LysVal: 4.316 ± 0.082
0.752LysTrp: 0.752 ± 0.026
2.849LysTyr: 2.849 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
6.451LeuAla: 6.451 ± 0.078
0.716LeuCys: 0.716 ± 0.03
4.744LeuAsp: 4.744 ± 0.076
5.432LeuGlu: 5.432 ± 0.084
4.5LeuPhe: 4.5 ± 0.078
5.451LeuGly: 5.451 ± 0.081
1.59LeuHis: 1.59 ± 0.037
5.701LeuIle: 5.701 ± 0.097
6.408LeuLys: 6.408 ± 0.098
8.712LeuLeu: 8.712 ± 0.13
2.122LeuMet: 2.122 ± 0.049
4.629LeuAsn: 4.629 ± 0.066
3.813LeuPro: 3.813 ± 0.069
3.197LeuGln: 3.197 ± 0.057
3.311LeuArg: 3.311 ± 0.062
6.254LeuSer: 6.254 ± 0.074
5.414LeuThr: 5.414 ± 0.09
5.095LeuVal: 5.095 ± 0.073
0.814LeuTrp: 0.814 ± 0.028
3.376LeuTyr: 3.376 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.053MetAla: 2.053 ± 0.046
0.128MetCys: 0.128 ± 0.01
1.333MetAsp: 1.333 ± 0.036
1.672MetGlu: 1.672 ± 0.039
0.867MetPhe: 0.867 ± 0.031
1.653MetGly: 1.653 ± 0.042
0.473MetHis: 0.473 ± 0.02
1.432MetIle: 1.432 ± 0.031
2.293MetLys: 2.293 ± 0.049
2.187MetLeu: 2.187 ± 0.044
0.661MetMet: 0.661 ± 0.025
1.215MetAsn: 1.215 ± 0.032
1.106MetPro: 1.106 ± 0.031
0.89MetGln: 0.89 ± 0.03
0.954MetArg: 0.954 ± 0.03
1.366MetSer: 1.366 ± 0.042
1.311MetThr: 1.311 ± 0.033
1.532MetVal: 1.532 ± 0.04
0.174MetTrp: 0.174 ± 0.013
0.783MetTyr: 0.783 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
4.234AsnAla: 4.234 ± 0.093
0.467AsnCys: 0.467 ± 0.022
3.202AsnAsp: 3.202 ± 0.06
3.048AsnGlu: 3.048 ± 0.053
2.814AsnPhe: 2.814 ± 0.052
4.437AsnGly: 4.437 ± 0.092
0.932AsnHis: 0.932 ± 0.028
4.315AsnIle: 4.315 ± 0.066
3.35AsnLys: 3.35 ± 0.06
4.563AsnLeu: 4.563 ± 0.07
1.263AsnMet: 1.263 ± 0.036
3.661AsnAsn: 3.661 ± 0.088
2.864AsnPro: 2.864 ± 0.061
1.604AsnGln: 1.604 ± 0.04
2.135AsnArg: 2.135 ± 0.048
3.348AsnSer: 3.348 ± 0.066
3.59AsnThr: 3.59 ± 0.071
3.574AsnVal: 3.574 ± 0.06
0.703AsnTrp: 0.703 ± 0.028
2.745AsnTyr: 2.745 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
3.341ProAla: 3.341 ± 0.08
0.226ProCys: 0.226 ± 0.017
2.47ProAsp: 2.47 ± 0.058
2.982ProGlu: 2.982 ± 0.055
1.882ProPhe: 1.882 ± 0.042
2.738ProGly: 2.738 ± 0.066
0.622ProHis: 0.622 ± 0.026
2.201ProIle: 2.201 ± 0.048
2.324ProLys: 2.324 ± 0.055
3.183ProLeu: 3.183 ± 0.062
0.882ProMet: 0.882 ± 0.027
2.087ProAsn: 2.087 ± 0.047
1.049ProPro: 1.049 ± 0.037
1.295ProGln: 1.295 ± 0.032
1.0ProArg: 1.0 ± 0.029
2.108ProSer: 2.108 ± 0.043
2.062ProThr: 2.062 ± 0.082
3.25ProVal: 3.25 ± 0.064
0.351ProTrp: 0.351 ± 0.017
1.606ProTyr: 1.606 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.369GlnAla: 2.369 ± 0.048
0.22GlnCys: 0.22 ± 0.018
1.695GlnAsp: 1.695 ± 0.04
2.106GlnGlu: 2.106 ± 0.048
1.554GlnPhe: 1.554 ± 0.038
1.955GlnGly: 1.955 ± 0.047
0.613GlnHis: 0.613 ± 0.024
2.023GlnIle: 2.023 ± 0.043
2.622GlnLys: 2.622 ± 0.062
3.155GlnLeu: 3.155 ± 0.054
0.92GlnMet: 0.92 ± 0.033
1.925GlnAsn: 1.925 ± 0.045
1.319GlnPro: 1.319 ± 0.044
1.54GlnGln: 1.54 ± 0.042
1.286GlnArg: 1.286 ± 0.039
1.836GlnSer: 1.836 ± 0.046
1.803GlnThr: 1.803 ± 0.052
2.088GlnVal: 2.088 ± 0.05
0.409GlnTrp: 0.409 ± 0.02
1.424GlnTyr: 1.424 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
2.232ArgAla: 2.232 ± 0.048
0.203ArgCys: 0.203 ± 0.014
1.875ArgAsp: 1.875 ± 0.044
2.521ArgGlu: 2.521 ± 0.06
1.931ArgPhe: 1.931 ± 0.048
1.95ArgGly: 1.95 ± 0.047
0.774ArgHis: 0.774 ± 0.027
2.933ArgIle: 2.933 ± 0.052
2.948ArgLys: 2.948 ± 0.059
3.42ArgLeu: 3.42 ± 0.062
1.08ArgMet: 1.08 ± 0.032
2.162ArgAsn: 2.162 ± 0.044
1.271ArgPro: 1.271 ± 0.037
1.373ArgGln: 1.373 ± 0.035
1.491ArgArg: 1.491 ± 0.039
2.023ArgSer: 2.023 ± 0.046
1.906ArgThr: 1.906 ± 0.037
2.213ArgVal: 2.213 ± 0.055
0.404ArgTrp: 0.404 ± 0.018
1.657ArgTyr: 1.657 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
4.524SerAla: 4.524 ± 0.095
0.631SerCys: 0.631 ± 0.028
3.414SerAsp: 3.414 ± 0.056
3.265SerGlu: 3.265 ± 0.061
3.476SerPhe: 3.476 ± 0.06
5.406SerGly: 5.406 ± 0.105
1.1SerHis: 1.1 ± 0.031
4.301SerIle: 4.301 ± 0.07
3.658SerLys: 3.658 ± 0.068
5.652SerLeu: 5.652 ± 0.078
1.335SerMet: 1.335 ± 0.037
3.269SerAsn: 3.269 ± 0.059
2.424SerPro: 2.424 ± 0.051
2.135SerGln: 2.135 ± 0.049
2.255SerArg: 2.255 ± 0.051
3.791SerSer: 3.791 ± 0.072
3.412SerThr: 3.412 ± 0.085
4.174SerVal: 4.174 ± 0.063
0.691SerTrp: 0.691 ± 0.026
2.688SerTyr: 2.688 ± 0.055
0.0SerXaa: 0.0 ± 0.0
Thr
5.372ThrAla: 5.372 ± 0.155
0.461ThrCys: 0.461 ± 0.026
3.644ThrAsp: 3.644 ± 0.061
3.47ThrGlu: 3.47 ± 0.06
3.137ThrPhe: 3.137 ± 0.066
5.498ThrGly: 5.498 ± 0.151
0.965ThrHis: 0.965 ± 0.027
4.973ThrIle: 4.973 ± 0.091
3.318ThrLys: 3.318 ± 0.058
5.541ThrLeu: 5.541 ± 0.096
1.139ThrMet: 1.139 ± 0.032
3.089ThrAsn: 3.089 ± 0.078
2.965ThrPro: 2.965 ± 0.091
1.992ThrGln: 1.992 ± 0.054
1.777ThrArg: 1.777 ± 0.038
3.925ThrSer: 3.925 ± 0.105
4.424ThrThr: 4.424 ± 0.162
4.905ThrVal: 4.905 ± 0.148
0.637ThrTrp: 0.637 ± 0.028
2.586ThrTyr: 2.586 ± 0.066
0.0ThrXaa: 0.0 ± 0.0
Val
5.035ValAla: 5.035 ± 0.095
0.577ValCys: 0.577 ± 0.023
3.306ValAsp: 3.306 ± 0.055
3.478ValGlu: 3.478 ± 0.059
3.209ValPhe: 3.209 ± 0.053
3.677ValGly: 3.677 ± 0.074
1.04ValHis: 1.04 ± 0.028
4.88ValIle: 4.88 ± 0.067
4.429ValLys: 4.429 ± 0.079
5.816ValLeu: 5.816 ± 0.079
1.52ValMet: 1.52 ± 0.039
3.744ValAsn: 3.744 ± 0.071
2.595ValPro: 2.595 ± 0.055
1.973ValGln: 1.973 ± 0.047
2.264ValArg: 2.264 ± 0.05
4.393ValSer: 4.393 ± 0.069
4.889ValThr: 4.889 ± 0.147
4.494ValVal: 4.494 ± 0.077
0.628ValTrp: 0.628 ± 0.025
2.695ValTyr: 2.695 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.709TrpAla: 0.709 ± 0.03
0.104TrpCys: 0.104 ± 0.01
0.681TrpAsp: 0.681 ± 0.028
0.654TrpGlu: 0.654 ± 0.026
0.535TrpPhe: 0.535 ± 0.024
0.705TrpGly: 0.705 ± 0.025
0.284TrpHis: 0.284 ± 0.016
0.682TrpIle: 0.682 ± 0.022
0.749TrpLys: 0.749 ± 0.023
0.956TrpLeu: 0.956 ± 0.03
0.31TrpMet: 0.31 ± 0.017
0.694TrpAsn: 0.694 ± 0.025
0.269TrpPro: 0.269 ± 0.019
0.448TrpGln: 0.448 ± 0.018
0.406TrpArg: 0.406 ± 0.018
0.596TrpSer: 0.596 ± 0.026
0.565TrpThr: 0.565 ± 0.03
0.658TrpVal: 0.658 ± 0.024
0.126TrpTrp: 0.126 ± 0.012
0.476TrpTyr: 0.476 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.796TyrAla: 2.796 ± 0.049
0.385TyrCys: 0.385 ± 0.023
2.441TyrAsp: 2.441 ± 0.049
2.298TyrGlu: 2.298 ± 0.045
2.511TyrPhe: 2.511 ± 0.055
2.732TyrGly: 2.732 ± 0.06
0.852TyrHis: 0.852 ± 0.029
2.883TyrIle: 2.883 ± 0.054
2.839TyrLys: 2.839 ± 0.053
3.591TyrLeu: 3.591 ± 0.061
0.898TyrMet: 0.898 ± 0.024
2.674TyrAsn: 2.674 ± 0.054
1.63TyrPro: 1.63 ± 0.036
1.404TyrGln: 1.404 ± 0.04
1.805TyrArg: 1.805 ± 0.041
2.877TyrSer: 2.877 ± 0.057
2.961TyrThr: 2.961 ± 0.075
2.326TyrVal: 2.326 ± 0.049
0.483TyrTrp: 0.483 ± 0.021
2.171TyrTyr: 2.171 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3477 proteins (1167212 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski