Caudovirales sp. ctOwN3
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W239|A0A5Q2W239_9CAUD Uncharacterized protein OS=Caudovirales sp. ctOwN3 OX=2656725 PE=4 SV=1
MM1 pKa = 7.56 SYY3 pKa = 11.36 VDD5 pKa = 4.18 VFTGSTIYY13 pKa = 10.23 PSEE16 pKa = 4.13 VSLTKK21 pKa = 10.37 LDD23 pKa = 3.77 LVANVILYY31 pKa = 8.5 WPLEE35 pKa = 4.1 AANGVPVASDD45 pKa = 2.72 IVEE48 pKa = 4.03 ITTATSASWTITLPDD63 pKa = 3.69 ATNGSLGEE71 pKa = 4.22 TILFNNLSAYY81 pKa = 10.49 SITVKK86 pKa = 10.4 NASGTTILSVTSGTQWQVYY105 pKa = 7.05 LHH107 pKa = 6.74 NNTTVAGGWRR117 pKa = 11.84 VYY119 pKa = 10.57 QFGAATSTANAASLVGPGLQASGSTLEE146 pKa = 4.25 TTIVTTNATVDD157 pKa = 3.74 TTFVTADD164 pKa = 3.53 RR165 pKa = 11.84 AKK167 pKa = 10.44 LYY169 pKa = 10.13 NWEE172 pKa = 4.14 GALGTFTLPNPAVVGNDD189 pKa = 2.7 WYY191 pKa = 11.69 VLVRR195 pKa = 11.84 NSGTGEE201 pKa = 3.96 LTIDD205 pKa = 4.14 PPLTAQINGSSTLQMAIGDD224 pKa = 4.26 SATIITDD231 pKa = 3.32 GTNFFTIGLGQSATFAFDD249 pKa = 3.51 YY250 pKa = 10.82 VVVDD254 pKa = 3.39 ISGSTDD260 pKa = 3.2 YY261 pKa = 10.54 TLSGSEE267 pKa = 4.37 LNRR270 pKa = 11.84 IAYY273 pKa = 8.59 QFIGAVGADD282 pKa = 3.05 ISVIVPNTTQQYY294 pKa = 7.55 WVYY297 pKa = 11.12 DD298 pKa = 3.54 NTTGGFTVSIGTATQVSPLTMVNGTRR324 pKa = 11.84 TIVYY328 pKa = 10.0 SDD330 pKa = 3.36 GTNVVPAVTSFVTGAIDD347 pKa = 4.0 GGTFF351 pKa = 3.02
Molecular weight: 36.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.808
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.554
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.897
Patrickios 0.299
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A5Q2W7G7|A0A5Q2W7G7_9CAUD Uncharacterized protein OS=Caudovirales sp. ctOwN3 OX=2656725 PE=4 SV=1
MM1 pKa = 7.2 RR2 pKa = 11.84 QKK4 pKa = 10.85 GSKK7 pKa = 9.38 KK8 pKa = 9.99 RR9 pKa = 11.84 LPIYY13 pKa = 10.46 VKK15 pKa = 9.76 DD16 pKa = 3.77 YY17 pKa = 9.66 YY18 pKa = 10.46 IVVVVSRR25 pKa = 11.84 LMLISADD32 pKa = 3.58 MLILFLYY39 pKa = 8.15 MGCNRR44 pKa = 11.84 QSLTRR49 pKa = 11.84 ARR51 pKa = 11.84 IVLARR56 pKa = 11.84 GFFRR60 pKa = 11.84 ISTAMLILFTRR71 pKa = 11.84 MSLLLYY77 pKa = 10.3 NAFAFWLYY85 pKa = 10.33 GAKK88 pKa = 9.5 SWQKK92 pKa = 10.72 INN94 pKa = 3.45
Molecular weight: 11.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 10.335
IPC_protein 11.067
Toseland 10.95
ProMoST 10.789
Dawson 11.052
Bjellqvist 10.833
Wikipedia 11.316
Rodwell 11.242
Grimsley 11.111
Solomon 11.213
Lehninger 11.169
Nozaki 10.921
DTASelect 10.833
Thurlkill 10.95
EMBOSS 11.359
Sillero 10.994
Patrickios 10.979
IPC_peptide 11.213
IPC2_peptide 9.926
IPC2.peptide.svr19 7.75
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
17551
33
1438
258.1
28.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.15 ± 0.478
0.775 ± 0.132
5.498 ± 0.258
5.92 ± 0.422
3.555 ± 0.209
8.341 ± 0.428
1.738 ± 0.198
5.293 ± 0.197
5.852 ± 0.398
7.789 ± 0.256
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.61 ± 0.161
4.484 ± 0.221
4.581 ± 0.199
4.49 ± 0.351
4.797 ± 0.266
6.37 ± 0.303
6.945 ± 0.57
6.495 ± 0.288
1.39 ± 0.147
3.926 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here