Moraxella catarrhalis (strain BBH18)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1881 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5VBP6|D5VBP6_MORCB Cell division protein ZapE OS=Moraxella catarrhalis (strain BBH18) OX=1236608 GN=zapE PE=3 SV=1
MM1 pKa = 8.31 IEE3 pKa = 4.0 ISSEE7 pKa = 3.99 LKK9 pKa = 10.15 QEE11 pKa = 4.18 MYY13 pKa = 10.93 HH14 pKa = 6.56 DD15 pKa = 4.79 AKK17 pKa = 11.1 NLWFSALFEE26 pKa = 5.2 NIPASLPNITFEE38 pKa = 4.09 EE39 pKa = 4.28 QKK41 pKa = 11.39 ALFFMLTKK49 pKa = 10.26 EE50 pKa = 4.47 ALDD53 pKa = 3.87 ADD55 pKa = 5.12 LIKK58 pKa = 10.53 FIPPNGIWYY67 pKa = 9.22 EE68 pKa = 4.42 GYY70 pKa = 10.55 DD71 pKa = 3.24 IWDD74 pKa = 3.57 VSSDD78 pKa = 3.3 EE79 pKa = 4.11 MVAYY83 pKa = 10.65 LRR85 pKa = 11.84 DD86 pKa = 4.15 NFPKK90 pKa = 10.72 DD91 pKa = 3.23 ATDD94 pKa = 4.45 GLDD97 pKa = 3.37 SCVNNYY103 pKa = 9.93 FYY105 pKa = 10.8 DD106 pKa = 3.63 IAPAVLWRR114 pKa = 11.84 QDD116 pKa = 3.2 DD117 pKa = 3.71 GSYY120 pKa = 10.8 YY121 pKa = 10.97 GSS123 pKa = 3.51
Molecular weight: 14.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.961
IPC_protein 3.923
Toseland 3.719
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.846
Rodwell 3.745
Grimsley 3.63
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.24
Thurlkill 3.77
EMBOSS 3.846
Sillero 4.037
Patrickios 1.914
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.937
Protein with the highest isoelectric point:
>tr|D5V9D3|D5V9D3_MORCB Valine-pyruvate aminotransferase OS=Moraxella catarrhalis (strain BBH18) OX=1236608 GN=avtA PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.32 KK26 pKa = 10.31 GRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.03 GRR39 pKa = 11.84 HH40 pKa = 5.34 RR41 pKa = 11.84 LTVV44 pKa = 3.07
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1881
0
1881
532181
37
2090
282.9
31.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.961 ± 0.069
1.001 ± 0.019
5.959 ± 0.05
4.959 ± 0.06
3.991 ± 0.043
6.791 ± 0.058
2.775 ± 0.036
7.169 ± 0.052
5.546 ± 0.047
10.142 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.806 ± 0.033
4.475 ± 0.051
3.907 ± 0.036
4.646 ± 0.047
4.32 ± 0.048
5.905 ± 0.039
5.837 ± 0.043
6.522 ± 0.053
1.144 ± 0.026
3.144 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here