Aeromonas phage 25AhydR2PP
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1PFQ3|A0A2S1PFQ3_9CAUD Putative tail fiber protein OS=Aeromonas phage 25AhydR2PP OX=2163976 PE=4 SV=1
MM1 pKa = 7.6 IPDD4 pKa = 4.26 YY5 pKa = 11.27 LPDD8 pKa = 4.52 CLDD11 pKa = 3.44 SVLWLIAPSVTLLQILAYY29 pKa = 10.28 GGLTLVAALAIIGSYY44 pKa = 10.97 LEE46 pKa = 4.59 DD47 pKa = 3.46 NQRR50 pKa = 11.84 RR51 pKa = 11.84 KK52 pKa = 9.76 PP53 pKa = 3.53
Molecular weight: 5.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.059
IPC2_protein 4.52
IPC_protein 4.177
Toseland 3.973
ProMoST 4.393
Dawson 4.19
Bjellqvist 4.355
Wikipedia 4.19
Rodwell 4.012
Grimsley 3.897
Solomon 4.164
Lehninger 4.126
Nozaki 4.342
DTASelect 4.584
Thurlkill 4.075
EMBOSS 4.19
Sillero 4.304
Patrickios 2.015
IPC_peptide 4.164
IPC2_peptide 4.279
IPC2.peptide.svr19 4.226
Protein with the highest isoelectric point:
>tr|A0A2S1PFT4|A0A2S1PFT4_9CAUD Putative DNA endonuclease OS=Aeromonas phage 25AhydR2PP OX=2163976 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.33 VLNRR6 pKa = 11.84 AYY8 pKa = 9.74 KK9 pKa = 10.29 LKK11 pKa = 10.85 ARR13 pKa = 11.84 AEE15 pKa = 4.23 RR16 pKa = 11.84 MLSAMWKK23 pKa = 9.81 RR24 pKa = 11.84 LHH26 pKa = 6.19 KK27 pKa = 10.1 WGEE30 pKa = 3.99 GYY32 pKa = 9.32 EE33 pKa = 4.17 RR34 pKa = 11.84 KK35 pKa = 10.1 HH36 pKa = 4.86 IARR39 pKa = 11.84 RR40 pKa = 11.84 SVVVVLASPLILFEE54 pKa = 4.43 MGRR57 pKa = 11.84 QAYY60 pKa = 9.16 HH61 pKa = 6.78 AVAEE65 pKa = 4.69 CAAEE69 pKa = 4.48 LYY71 pKa = 10.59 QYY73 pKa = 10.34 IRR75 pKa = 11.84 HH76 pKa = 5.93 NNN78 pKa = 3.23
Molecular weight: 9.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.294
IPC2_protein 9.765
IPC_protein 10.306
Toseland 10.335
ProMoST 10.058
Dawson 10.526
Bjellqvist 10.233
Wikipedia 10.716
Rodwell 10.847
Grimsley 10.599
Solomon 10.57
Lehninger 10.54
Nozaki 10.321
DTASelect 10.218
Thurlkill 10.379
EMBOSS 10.745
Sillero 10.438
Patrickios 10.57
IPC_peptide 10.57
IPC2_peptide 9.107
IPC2.peptide.svr19 8.58
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
13215
43
1250
259.1
28.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.898 ± 0.553
1.158 ± 0.163
5.986 ± 0.198
6.599 ± 0.272
3.405 ± 0.184
7.537 ± 0.304
2.217 ± 0.199
4.555 ± 0.275
6.16 ± 0.291
8.468 ± 0.309
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.284 ± 0.165
3.731 ± 0.18
3.912 ± 0.205
4.397 ± 0.33
5.728 ± 0.204
6.114 ± 0.36
5.131 ± 0.217
6.977 ± 0.307
1.476 ± 0.127
3.269 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here