Amino acid dipepetide frequency for Aeromonas phage 25AhydR2PP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.352AlaAla: 11.352 ± 1.187
0.984AlaCys: 0.984 ± 0.301
5.903AlaAsp: 5.903 ± 0.763
8.097AlaGlu: 8.097 ± 0.753
3.633AlaPhe: 3.633 ± 0.473
7.87AlaGly: 7.87 ± 0.706
1.892AlaHis: 1.892 ± 0.339
3.86AlaIle: 3.86 ± 0.63
6.206AlaLys: 6.206 ± 0.639
8.93AlaLeu: 8.93 ± 1.048
2.8AlaMet: 2.8 ± 0.507
3.254AlaAsn: 3.254 ± 0.487
2.876AlaPro: 2.876 ± 0.5
3.935AlaGln: 3.935 ± 0.744
6.054AlaArg: 6.054 ± 0.599
4.541AlaSer: 4.541 ± 0.599
4.314AlaThr: 4.314 ± 0.761
7.643AlaVal: 7.643 ± 0.871
0.984AlaTrp: 0.984 ± 0.224
3.86AlaTyr: 3.86 ± 0.512
0.0AlaXaa: 0.0 ± 0.0
Cys
0.984CysAla: 0.984 ± 0.294
0.151CysCys: 0.151 ± 0.087
0.832CysAsp: 0.832 ± 0.243
0.757CysGlu: 0.757 ± 0.24
0.454CysPhe: 0.454 ± 0.171
0.757CysGly: 0.757 ± 0.249
0.303CysHis: 0.303 ± 0.151
0.832CysIle: 0.832 ± 0.259
0.303CysLys: 0.303 ± 0.164
0.984CysLeu: 0.984 ± 0.251
0.378CysMet: 0.378 ± 0.187
0.378CysAsn: 0.378 ± 0.18
0.757CysPro: 0.757 ± 0.222
0.151CysGln: 0.151 ± 0.097
0.757CysArg: 0.757 ± 0.364
0.605CysSer: 0.605 ± 0.208
0.984CysThr: 0.984 ± 0.321
0.681CysVal: 0.681 ± 0.257
0.076CysTrp: 0.076 ± 0.077
0.454CysTyr: 0.454 ± 0.173
0.0CysXaa: 0.0 ± 0.0
Asp
6.433AspAla: 6.433 ± 0.686
0.832AspCys: 0.832 ± 0.241
2.951AspAsp: 2.951 ± 0.525
4.692AspGlu: 4.692 ± 0.604
2.497AspPhe: 2.497 ± 0.492
4.541AspGly: 4.541 ± 0.537
1.135AspHis: 1.135 ± 0.364
2.724AspIle: 2.724 ± 0.44
4.843AspLys: 4.843 ± 0.587
4.314AspLeu: 4.314 ± 0.684
2.119AspMet: 2.119 ± 0.423
2.724AspAsn: 2.724 ± 0.554
2.043AspPro: 2.043 ± 0.393
1.438AspGln: 1.438 ± 0.365
2.346AspArg: 2.346 ± 0.475
4.011AspSer: 4.011 ± 0.564
3.103AspThr: 3.103 ± 0.757
4.238AspVal: 4.238 ± 0.568
0.681AspTrp: 0.681 ± 0.235
2.195AspTyr: 2.195 ± 0.471
0.0AspXaa: 0.0 ± 0.0
Glu
5.449GluAla: 5.449 ± 0.552
1.135GluCys: 1.135 ± 0.318
3.86GluAsp: 3.86 ± 0.47
4.011GluGlu: 4.011 ± 0.581
2.649GluPhe: 2.649 ± 0.475
4.238GluGly: 4.238 ± 0.445
1.589GluHis: 1.589 ± 0.319
2.27GluIle: 2.27 ± 0.414
3.481GluLys: 3.481 ± 0.571
6.584GluLeu: 6.584 ± 0.588
2.573GluMet: 2.573 ± 0.473
1.589GluAsn: 1.589 ± 0.27
2.195GluPro: 2.195 ± 0.52
4.995GluGln: 4.995 ± 0.718
4.162GluArg: 4.162 ± 0.667
3.557GluSer: 3.557 ± 0.507
3.103GluThr: 3.103 ± 0.349
4.541GluVal: 4.541 ± 0.606
1.741GluTrp: 1.741 ± 0.378
2.195GluTyr: 2.195 ± 0.305
0.0GluXaa: 0.0 ± 0.0
Phe
2.649PheAla: 2.649 ± 0.345
0.454PheCys: 0.454 ± 0.201
2.649PheAsp: 2.649 ± 0.357
1.741PheGlu: 1.741 ± 0.503
1.059PhePhe: 1.059 ± 0.334
2.8PheGly: 2.8 ± 0.477
0.378PheHis: 0.378 ± 0.18
2.043PheIle: 2.043 ± 0.377
2.497PheLys: 2.497 ± 0.413
2.346PheLeu: 2.346 ± 0.387
0.908PheMet: 0.908 ± 0.275
2.195PheAsn: 2.195 ± 0.468
1.059PhePro: 1.059 ± 0.303
1.816PheGln: 1.816 ± 0.349
2.346PheArg: 2.346 ± 0.413
1.968PheSer: 1.968 ± 0.367
2.043PheThr: 2.043 ± 0.375
1.741PheVal: 1.741 ± 0.418
0.454PheTrp: 0.454 ± 0.212
0.908PheTyr: 0.908 ± 0.261
0.0PheXaa: 0.0 ± 0.0
Gly
5.903GlyAla: 5.903 ± 0.694
1.135GlyCys: 1.135 ± 0.397
5.373GlyAsp: 5.373 ± 0.582
3.557GlyGlu: 3.557 ± 0.43
3.027GlyPhe: 3.027 ± 0.418
6.584GlyGly: 6.584 ± 0.801
1.665GlyHis: 1.665 ± 0.331
3.708GlyIle: 3.708 ± 0.527
5.297GlyLys: 5.297 ± 0.76
5.903GlyLeu: 5.903 ± 0.817
1.968GlyMet: 1.968 ± 0.347
3.254GlyAsn: 3.254 ± 0.473
2.043GlyPro: 2.043 ± 0.323
3.027GlyGln: 3.027 ± 0.598
4.919GlyArg: 4.919 ± 0.501
4.768GlySer: 4.768 ± 0.691
4.843GlyThr: 4.843 ± 0.626
5.449GlyVal: 5.449 ± 0.595
0.908GlyTrp: 0.908 ± 0.263
2.043GlyTyr: 2.043 ± 0.516
0.0GlyXaa: 0.0 ± 0.0
His
1.211HisAla: 1.211 ± 0.318
0.303HisCys: 0.303 ± 0.162
1.816HisAsp: 1.816 ± 0.348
1.059HisGlu: 1.059 ± 0.367
0.832HisPhe: 0.832 ± 0.244
1.287HisGly: 1.287 ± 0.368
0.605HisHis: 0.605 ± 0.221
1.211HisIle: 1.211 ± 0.293
1.135HisLys: 1.135 ± 0.258
1.968HisLeu: 1.968 ± 0.368
1.135HisMet: 1.135 ± 0.321
0.605HisAsn: 0.605 ± 0.203
1.287HisPro: 1.287 ± 0.389
0.681HisGln: 0.681 ± 0.227
1.438HisArg: 1.438 ± 0.309
0.984HisSer: 0.984 ± 0.287
1.211HisThr: 1.211 ± 0.28
1.665HisVal: 1.665 ± 0.346
0.454HisTrp: 0.454 ± 0.152
1.287HisTyr: 1.287 ± 0.345
0.0HisXaa: 0.0 ± 0.0
Ile
4.389IleAla: 4.389 ± 0.579
0.378IleCys: 0.378 ± 0.17
2.119IleAsp: 2.119 ± 0.361
3.33IleGlu: 3.33 ± 0.42
0.908IlePhe: 0.908 ± 0.36
3.103IleGly: 3.103 ± 0.485
1.514IleHis: 1.514 ± 0.306
2.195IleIle: 2.195 ± 0.566
2.951IleLys: 2.951 ± 0.389
2.951IleLeu: 2.951 ± 0.402
1.665IleMet: 1.665 ± 0.305
2.27IleAsn: 2.27 ± 0.523
1.968IlePro: 1.968 ± 0.32
2.043IleGln: 2.043 ± 0.425
3.784IleArg: 3.784 ± 0.432
3.027IleSer: 3.027 ± 0.645
2.497IleThr: 2.497 ± 0.487
2.422IleVal: 2.422 ± 0.427
0.605IleTrp: 0.605 ± 0.225
1.438IleTyr: 1.438 ± 0.31
0.0IleXaa: 0.0 ± 0.0
Lys
7.643LysAla: 7.643 ± 0.861
0.227LysCys: 0.227 ± 0.138
3.557LysAsp: 3.557 ± 0.663
4.692LysGlu: 4.692 ± 0.615
2.27LysPhe: 2.27 ± 0.431
4.692LysGly: 4.692 ± 0.664
1.287LysHis: 1.287 ± 0.33
2.497LysIle: 2.497 ± 0.531
3.254LysLys: 3.254 ± 0.592
6.13LysLeu: 6.13 ± 0.592
2.8LysMet: 2.8 ± 0.415
1.589LysAsn: 1.589 ± 0.403
2.951LysPro: 2.951 ± 0.416
2.951LysGln: 2.951 ± 0.593
2.649LysArg: 2.649 ± 0.437
2.573LysSer: 2.573 ± 0.451
2.497LysThr: 2.497 ± 0.552
4.314LysVal: 4.314 ± 0.628
1.211LysTrp: 1.211 ± 0.33
1.816LysTyr: 1.816 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
8.854LeuAla: 8.854 ± 0.804
0.832LeuCys: 0.832 ± 0.313
6.206LeuAsp: 6.206 ± 0.58
4.843LeuGlu: 4.843 ± 0.749
1.892LeuPhe: 1.892 ± 0.31
5.903LeuGly: 5.903 ± 0.614
1.816LeuHis: 1.816 ± 0.3
3.405LeuIle: 3.405 ± 0.511
5.07LeuLys: 5.07 ± 0.604
6.281LeuLeu: 6.281 ± 0.825
2.8LeuMet: 2.8 ± 0.465
3.254LeuAsn: 3.254 ± 0.449
4.011LeuPro: 4.011 ± 0.478
3.935LeuGln: 3.935 ± 0.631
4.314LeuArg: 4.314 ± 0.533
7.114LeuSer: 7.114 ± 0.705
5.524LeuThr: 5.524 ± 0.657
5.222LeuVal: 5.222 ± 0.693
0.832LeuTrp: 0.832 ± 0.256
2.573LeuTyr: 2.573 ± 0.527
0.0LeuXaa: 0.0 ± 0.0
Met
4.011MetAla: 4.011 ± 0.702
0.076MetCys: 0.076 ± 0.088
1.968MetAsp: 1.968 ± 0.446
2.195MetGlu: 2.195 ± 0.402
1.059MetPhe: 1.059 ± 0.261
1.816MetGly: 1.816 ± 0.362
1.135MetHis: 1.135 ± 0.281
1.135MetIle: 1.135 ± 0.204
2.27MetLys: 2.27 ± 0.45
3.103MetLeu: 3.103 ± 0.482
0.681MetMet: 0.681 ± 0.222
0.984MetAsn: 0.984 ± 0.282
1.211MetPro: 1.211 ± 0.352
1.665MetGln: 1.665 ± 0.407
2.119MetArg: 2.119 ± 0.355
2.724MetSer: 2.724 ± 0.313
1.438MetThr: 1.438 ± 0.347
2.27MetVal: 2.27 ± 0.419
0.454MetTrp: 0.454 ± 0.147
0.53MetTyr: 0.53 ± 0.168
0.0MetXaa: 0.0 ± 0.0
Asn
3.178AsnAla: 3.178 ± 0.414
0.681AsnCys: 0.681 ± 0.239
1.135AsnAsp: 1.135 ± 0.255
1.892AsnGlu: 1.892 ± 0.296
1.287AsnPhe: 1.287 ± 0.25
3.405AsnGly: 3.405 ± 0.46
0.984AsnHis: 0.984 ± 0.314
1.968AsnIle: 1.968 ± 0.409
2.649AsnLys: 2.649 ± 0.349
3.481AsnLeu: 3.481 ± 0.46
1.514AsnMet: 1.514 ± 0.408
1.211AsnAsn: 1.211 ± 0.271
2.497AsnPro: 2.497 ± 0.471
1.362AsnGln: 1.362 ± 0.289
2.27AsnArg: 2.27 ± 0.408
1.816AsnSer: 1.816 ± 0.421
2.043AsnThr: 2.043 ± 0.297
2.27AsnVal: 2.27 ± 0.512
0.454AsnTrp: 0.454 ± 0.17
1.211AsnTyr: 1.211 ± 0.339
0.0AsnXaa: 0.0 ± 0.0
Pro
3.481ProAla: 3.481 ± 0.795
0.303ProCys: 0.303 ± 0.126
3.027ProAsp: 3.027 ± 0.43
3.708ProGlu: 3.708 ± 0.492
1.287ProPhe: 1.287 ± 0.304
2.573ProGly: 2.573 ± 0.442
0.832ProHis: 0.832 ± 0.221
2.119ProIle: 2.119 ± 0.423
2.346ProLys: 2.346 ± 0.371
3.254ProLeu: 3.254 ± 0.492
1.287ProMet: 1.287 ± 0.277
1.211ProAsn: 1.211 ± 0.349
1.362ProPro: 1.362 ± 0.288
1.589ProGln: 1.589 ± 0.276
0.908ProArg: 0.908 ± 0.304
3.103ProSer: 3.103 ± 0.426
1.741ProThr: 1.741 ± 0.318
3.33ProVal: 3.33 ± 0.477
0.378ProTrp: 0.378 ± 0.164
1.287ProTyr: 1.287 ± 0.304
0.0ProXaa: 0.0 ± 0.0
Gln
4.995GlnAla: 4.995 ± 0.716
0.378GlnCys: 0.378 ± 0.158
2.27GlnAsp: 2.27 ± 0.495
3.254GlnGlu: 3.254 ± 0.597
1.287GlnPhe: 1.287 ± 0.317
3.481GlnGly: 3.481 ± 0.494
1.287GlnHis: 1.287 ± 0.23
2.422GlnIle: 2.422 ± 0.317
2.346GlnLys: 2.346 ± 0.46
4.314GlnLeu: 4.314 ± 0.5
1.741GlnMet: 1.741 ± 0.304
1.362GlnAsn: 1.362 ± 0.307
0.984GlnPro: 0.984 ± 0.309
1.892GlnGln: 1.892 ± 0.488
2.346GlnArg: 2.346 ± 0.481
2.119GlnSer: 2.119 ± 0.314
1.665GlnThr: 1.665 ± 0.369
2.951GlnVal: 2.951 ± 0.389
1.059GlnTrp: 1.059 ± 0.217
1.816GlnTyr: 1.816 ± 0.301
0.0GlnXaa: 0.0 ± 0.0
Arg
5.449ArgAla: 5.449 ± 0.614
0.53ArgCys: 0.53 ± 0.232
3.633ArgAsp: 3.633 ± 0.424
3.557ArgGlu: 3.557 ± 0.407
2.043ArgPhe: 2.043 ± 0.369
3.405ArgGly: 3.405 ± 0.42
1.665ArgHis: 1.665 ± 0.273
3.784ArgIle: 3.784 ± 0.539
3.103ArgLys: 3.103 ± 0.582
5.297ArgLeu: 5.297 ± 0.614
2.043ArgMet: 2.043 ± 0.446
2.649ArgAsn: 2.649 ± 0.41
1.589ArgPro: 1.589 ± 0.331
2.8ArgGln: 2.8 ± 0.574
3.557ArgArg: 3.557 ± 0.62
2.724ArgSer: 2.724 ± 0.395
2.649ArgThr: 2.649 ± 0.399
3.708ArgVal: 3.708 ± 0.516
0.984ArgTrp: 0.984 ± 0.318
2.119ArgTyr: 2.119 ± 0.367
0.0ArgXaa: 0.0 ± 0.0
Ser
5.297SerAla: 5.297 ± 0.631
0.454SerCys: 0.454 ± 0.168
3.405SerAsp: 3.405 ± 0.435
3.254SerGlu: 3.254 ± 0.511
1.968SerPhe: 1.968 ± 0.399
5.07SerGly: 5.07 ± 0.852
0.832SerHis: 0.832 ± 0.225
2.27SerIle: 2.27 ± 0.435
4.087SerLys: 4.087 ± 0.515
4.919SerLeu: 4.919 ± 0.71
1.741SerMet: 1.741 ± 0.469
3.103SerAsn: 3.103 ± 0.594
2.649SerPro: 2.649 ± 0.432
2.876SerGln: 2.876 ± 0.537
4.616SerArg: 4.616 ± 0.625
2.951SerSer: 2.951 ± 0.598
3.481SerThr: 3.481 ± 0.476
5.449SerVal: 5.449 ± 0.703
0.984SerTrp: 0.984 ± 0.283
1.741SerTyr: 1.741 ± 0.394
0.0SerXaa: 0.0 ± 0.0
Thr
6.66ThrAla: 6.66 ± 0.581
0.681ThrCys: 0.681 ± 0.251
2.195ThrAsp: 2.195 ± 0.372
3.33ThrGlu: 3.33 ± 0.52
1.135ThrPhe: 1.135 ± 0.375
4.919ThrGly: 4.919 ± 0.737
1.059ThrHis: 1.059 ± 0.247
1.816ThrIle: 1.816 ± 0.395
3.633ThrLys: 3.633 ± 0.509
4.011ThrLeu: 4.011 ± 0.582
1.059ThrMet: 1.059 ± 0.286
1.438ThrAsn: 1.438 ± 0.32
2.876ThrPro: 2.876 ± 0.418
2.119ThrGln: 2.119 ± 0.331
2.346ThrArg: 2.346 ± 0.472
3.86ThrSer: 3.86 ± 0.541
2.195ThrThr: 2.195 ± 0.364
3.405ThrVal: 3.405 ± 0.533
0.757ThrTrp: 0.757 ± 0.268
1.816ThrTyr: 1.816 ± 0.308
0.0ThrXaa: 0.0 ± 0.0
Val
6.811ValAla: 6.811 ± 0.714
0.984ValCys: 0.984 ± 0.291
3.784ValAsp: 3.784 ± 0.442
5.449ValGlu: 5.449 ± 0.705
2.951ValPhe: 2.951 ± 0.544
5.979ValGly: 5.979 ± 0.818
1.211ValHis: 1.211 ± 0.325
3.633ValIle: 3.633 ± 0.583
2.876ValLys: 2.876 ± 0.493
5.222ValLeu: 5.222 ± 0.483
2.119ValMet: 2.119 ± 0.317
2.497ValAsn: 2.497 ± 0.428
3.027ValPro: 3.027 ± 0.71
3.178ValGln: 3.178 ± 0.308
4.389ValArg: 4.389 ± 0.682
4.314ValSer: 4.314 ± 0.346
3.633ValThr: 3.633 ± 0.473
4.995ValVal: 4.995 ± 0.574
0.53ValTrp: 0.53 ± 0.188
2.195ValTyr: 2.195 ± 0.567
0.0ValXaa: 0.0 ± 0.0
Trp
1.589TrpAla: 1.589 ± 0.324
0.151TrpCys: 0.151 ± 0.104
1.287TrpAsp: 1.287 ± 0.424
0.908TrpGlu: 0.908 ± 0.221
0.908TrpPhe: 0.908 ± 0.255
0.454TrpGly: 0.454 ± 0.16
0.227TrpHis: 0.227 ± 0.117
0.227TrpIle: 0.227 ± 0.109
1.211TrpLys: 1.211 ± 0.298
1.741TrpLeu: 1.741 ± 0.513
0.605TrpMet: 0.605 ± 0.215
0.303TrpAsn: 0.303 ± 0.13
0.53TrpPro: 0.53 ± 0.208
0.53TrpGln: 0.53 ± 0.273
0.303TrpArg: 0.303 ± 0.141
1.135TrpSer: 1.135 ± 0.247
0.605TrpThr: 0.605 ± 0.195
0.984TrpVal: 0.984 ± 0.257
0.605TrpTrp: 0.605 ± 0.14
0.454TrpTyr: 0.454 ± 0.194
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.649TyrAla: 2.649 ± 0.417
0.908TyrCys: 0.908 ± 0.267
1.892TyrAsp: 1.892 ± 0.443
1.665TyrGlu: 1.665 ± 0.418
0.908TyrPhe: 0.908 ± 0.24
2.497TyrGly: 2.497 ± 0.426
0.757TyrHis: 0.757 ± 0.235
1.438TyrIle: 1.438 ± 0.359
2.043TyrLys: 2.043 ± 0.418
2.951TyrLeu: 2.951 ± 0.428
0.832TyrMet: 0.832 ± 0.283
1.438TyrAsn: 1.438 ± 0.277
1.211TyrPro: 1.211 ± 0.359
0.984TyrGln: 0.984 ± 0.246
1.665TyrArg: 1.665 ± 0.348
3.254TyrSer: 3.254 ± 0.7
1.741TyrThr: 1.741 ± 0.375
2.497TyrVal: 2.497 ± 0.399
0.605TyrTrp: 0.605 ± 0.205
0.757TyrTyr: 0.757 ± 0.215
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (13215 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski