Dishui Lake virophage 3
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6XN60|A0A6G6XN60_9VIRU Putative major capsid protein OS=Dishui Lake virophage 3 OX=2704066 PE=4 SV=1
MM1 pKa = 6.42 TTRR4 pKa = 11.84 GNLTSADD11 pKa = 4.25 PYY13 pKa = 10.97 NLYY16 pKa = 10.92 YY17 pKa = 10.57 DD18 pKa = 3.7 INVVSDD24 pKa = 3.82 YY25 pKa = 11.42 NPTLVGTTAPPLTFNEE41 pKa = 3.77 IRR43 pKa = 11.84 QNPIIKK49 pKa = 10.47 YY50 pKa = 9.54 PEE52 pKa = 5.12 DD53 pKa = 3.49 YY54 pKa = 10.51 LLSVVRR60 pKa = 11.84 FSIEE64 pKa = 4.22 TPTLPIFIPQVLLGQPNPNKK84 pKa = 10.47 LIYY87 pKa = 9.58 AWGMSVTDD95 pKa = 3.8 YY96 pKa = 11.18 SAVGTPTYY104 pKa = 10.48 YY105 pKa = 10.95 LPAQEE110 pKa = 3.88 NWIYY114 pKa = 10.77 IPDD117 pKa = 4.94 DD118 pKa = 3.48 LTIPPPQGALTFQDD132 pKa = 3.75 LTTEE136 pKa = 4.11 YY137 pKa = 10.48 YY138 pKa = 9.99 YY139 pKa = 11.55 VNEE142 pKa = 4.24 FSLILQYY149 pKa = 11.71 ANNALKK155 pKa = 10.6 AAFDD159 pKa = 3.76 NFNTYY164 pKa = 8.44 LTGIGQPTLGAFGGVPANIAQNYY187 pKa = 7.5 CPQMTYY193 pKa = 11.01 DD194 pKa = 3.52 PRR196 pKa = 11.84 GEE198 pKa = 4.11 LFSLSFPLCPPALAGAAPPFAYY220 pKa = 8.5 DD221 pKa = 3.78 TYY223 pKa = 11.34 DD224 pKa = 3.26 QNLANTAGFAGRR236 pKa = 11.84 VIKK239 pKa = 10.66 LYY241 pKa = 10.72 MNTPLSNLLNSFPTVFQGNQQFQLTTGTEE270 pKa = 4.04 DD271 pKa = 3.41 MIVVYY276 pKa = 10.69 NNQYY280 pKa = 10.85 QNTNGGSRR288 pKa = 11.84 PTYY291 pKa = 9.41 PLTPVASTVAAIPQIIVPQEE311 pKa = 3.66 HH312 pKa = 6.34 STTILFSPISSLVFSTSLLPVQNTLLSKK340 pKa = 9.79 PAIFNFYY347 pKa = 11.07 DD348 pKa = 3.65 GVTSSNLRR356 pKa = 11.84 SSGNNNVTAPVLTDD370 pKa = 4.13 FEE372 pKa = 4.72 LQGATGTSSQTRR384 pKa = 11.84 ITYY387 pKa = 8.39 TPTAEE392 pKa = 3.92 YY393 pKa = 11.03 RR394 pKa = 11.84 MLDD397 pKa = 3.46 LRR399 pKa = 11.84 GTTPVNAVEE408 pKa = 5.21 VSVFWKK414 pKa = 10.67 DD415 pKa = 3.22 KK416 pKa = 10.61 FSGLHH421 pKa = 5.86 RR422 pKa = 11.84 FNLAAGCAASIKK434 pKa = 10.3 ILFRR438 pKa = 11.84 RR439 pKa = 11.84 KK440 pKa = 10.0 DD441 pKa = 3.69 FYY443 pKa = 11.55 NATIDD448 pKa = 3.32
Molecular weight: 49.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.658
IPC2_protein 4.724
IPC_protein 4.647
Toseland 4.469
ProMoST 4.698
Dawson 4.609
Bjellqvist 4.787
Wikipedia 4.507
Rodwell 4.482
Grimsley 4.38
Solomon 4.609
Lehninger 4.558
Nozaki 4.724
DTASelect 4.927
Thurlkill 4.495
EMBOSS 4.52
Sillero 4.762
Patrickios 0.49
IPC_peptide 4.609
IPC2_peptide 4.749
IPC2.peptide.svr19 4.697
Protein with the highest isoelectric point:
>tr|A0A6G6XMN2|A0A6G6XMN2_9VIRU Uncharacterized protein OS=Dishui Lake virophage 3 OX=2704066 PE=4 SV=1
MM1 pKa = 7.18 ITQTLINAFFRR12 pKa = 11.84 VSPATTPSPPPSPDD26 pKa = 3.48 PLTDD30 pKa = 3.06 DD31 pKa = 3.13 WVVIDD36 pKa = 3.98 EE37 pKa = 4.42 RR38 pKa = 11.84 FGVRR42 pKa = 11.84 KK43 pKa = 10.06 DD44 pKa = 3.22 NYY46 pKa = 9.56 AINRR50 pKa = 11.84 IGQVKK55 pKa = 9.59 NLKK58 pKa = 9.73 FNKK61 pKa = 9.33 ILKK64 pKa = 10.29 SYY66 pKa = 8.17 FCKK69 pKa = 10.57 SIGYY73 pKa = 8.32 MCVSLKK79 pKa = 10.73 RR80 pKa = 11.84 SMDD83 pKa = 3.11 ASGNLVRR90 pKa = 11.84 PNNGYY95 pKa = 9.37 GRR97 pKa = 11.84 KK98 pKa = 8.67 PVKK101 pKa = 9.47 DD102 pKa = 3.56 TPNNEE107 pKa = 3.77 TLMLVHH113 pKa = 6.96 RR114 pKa = 11.84 LVGNVFIPNTNPNYY128 pKa = 10.46 RR129 pKa = 11.84 IIDD132 pKa = 4.31 HH133 pKa = 7.24 IDD135 pKa = 3.28 GNKK138 pKa = 10.21 LNNDD142 pKa = 3.29 YY143 pKa = 11.33 RR144 pKa = 11.84 NLRR147 pKa = 11.84 WCDD150 pKa = 3.16 QRR152 pKa = 11.84 RR153 pKa = 11.84 NANNAKK159 pKa = 10.18 SNKK162 pKa = 9.34 KK163 pKa = 8.42 YY164 pKa = 10.13 WGVRR168 pKa = 11.84 WAKK171 pKa = 10.85 NMEE174 pKa = 4.04 KK175 pKa = 9.73 WSASIQTTVNYY186 pKa = 10.35 NPNEE190 pKa = 4.29 TFTHH194 pKa = 6.54 FLGHH198 pKa = 6.47 YY199 pKa = 9.19 DD200 pKa = 4.09 DD201 pKa = 5.23 EE202 pKa = 4.99 VMAARR207 pKa = 11.84 VVKK210 pKa = 10.64 KK211 pKa = 10.23 FMTEE215 pKa = 3.68 NYY217 pKa = 8.23 PNEE220 pKa = 4.08 MTGGRR225 pKa = 11.84 RR226 pKa = 11.84 FIEE229 pKa = 4.34 EE230 pKa = 3.77
Molecular weight: 26.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.945
IPC2_protein 9.092
IPC_protein 9.048
Toseland 9.692
ProMoST 9.458
Dawson 9.955
Bjellqvist 9.663
Wikipedia 10.131
Rodwell 10.277
Grimsley 10.028
Solomon 9.984
Lehninger 9.94
Nozaki 9.75
DTASelect 9.633
Thurlkill 9.794
EMBOSS 10.116
Sillero 9.882
Patrickios 5.537
IPC_peptide 9.984
IPC2_peptide 8.302
IPC2.peptide.svr19 8.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
4760
92
1726
396.7
45.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.227 ± 1.065
0.987 ± 0.31
6.282 ± 0.349
6.87 ± 1.37
4.055 ± 0.405
5.819 ± 0.327
1.492 ± 0.425
5.756 ± 0.6
7.941 ± 1.303
7.731 ± 0.652
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.878 ± 0.303
5.567 ± 0.864
4.958 ± 0.492
3.95 ± 0.483
5.693 ± 0.586
5.798 ± 0.7
5.945 ± 0.741
5.903 ± 0.298
1.239 ± 0.157
3.908 ± 0.427
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here