Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanosphaera; Methanosphaera stadtmanae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2NGA0|Q2NGA0_METST Putative bile salt acid hydrolase OS=Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3) OX=339860 GN=Msp_0760 PE=3 SV=1
MM1 pKa = 8.36VEE3 pKa = 3.82IVLDD7 pKa = 3.85RR8 pKa = 11.84DD9 pKa = 3.43EE10 pKa = 4.81CTSCGNCVEE19 pKa = 4.36VDD21 pKa = 3.65DD22 pKa = 5.8SLFTFDD28 pKa = 5.57SDD30 pKa = 5.43DD31 pKa = 3.58KK32 pKa = 10.97ATMIGSEE39 pKa = 4.21RR40 pKa = 11.84DD41 pKa = 3.28DD42 pKa = 3.53GMEE45 pKa = 4.4EE46 pKa = 4.59IEE48 pKa = 4.56VDD50 pKa = 4.98DD51 pKa = 3.68PTKK54 pKa = 10.64YY55 pKa = 10.15QEE57 pKa = 5.17AADD60 pKa = 3.9KK61 pKa = 11.25CKK63 pKa = 10.91GEE65 pKa = 4.41CIEE68 pKa = 5.06VYY70 pKa = 10.42DD71 pKa = 4.23

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q2NFX7|RS5_METST 30S ribosomal protein S5 OS=Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3) OX=339860 GN=rps5 PE=3 SV=1
MM1 pKa = 7.02ARR3 pKa = 11.84KK4 pKa = 9.22YY5 pKa = 10.75GKK7 pKa = 9.85AARR10 pKa = 11.84KK11 pKa = 9.25CSRR14 pKa = 11.84CGDD17 pKa = 3.15HH18 pKa = 6.44SAIVRR23 pKa = 11.84RR24 pKa = 11.84YY25 pKa = 9.53GLNLCRR31 pKa = 11.84QCFRR35 pKa = 11.84EE36 pKa = 4.01IAPKK40 pKa = 10.26IGFKK44 pKa = 10.41KK45 pKa = 10.7YY46 pKa = 9.54NN47 pKa = 3.3

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1533

0

1533

493630

37

3356

322.0

36.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.168 ± 0.079

1.174 ± 0.034

6.08 ± 0.053

6.795 ± 0.108

3.566 ± 0.052

5.93 ± 0.064

1.694 ± 0.03

10.314 ± 0.073

8.16 ± 0.074

8.367 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.039

7.512 ± 0.182

3.166 ± 0.045

2.586 ± 0.034

3.023 ± 0.058

6.266 ± 0.062

6.74 ± 0.157

6.302 ± 0.056

0.552 ± 0.017

4.329 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski