Amino acid dipepetide frequency for Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.227AlaAla: 3.227 ± 0.12
0.713AlaCys: 0.713 ± 0.045
2.8AlaAsp: 2.8 ± 0.099
2.741AlaGlu: 2.741 ± 0.089
1.815AlaPhe: 1.815 ± 0.066
3.476AlaGly: 3.476 ± 0.118
1.033AlaHis: 1.033 ± 0.049
5.49AlaIle: 5.49 ± 0.145
4.068AlaLys: 4.068 ± 0.111
4.783AlaLeu: 4.783 ± 0.132
1.315AlaMet: 1.315 ± 0.054
2.897AlaAsn: 2.897 ± 0.104
1.422AlaPro: 1.422 ± 0.056
1.671AlaGln: 1.671 ± 0.06
1.896AlaArg: 1.896 ± 0.06
3.339AlaSer: 3.339 ± 0.09
3.472AlaThr: 3.472 ± 0.104
3.369AlaVal: 3.369 ± 0.098
0.243AlaTrp: 0.243 ± 0.02
1.906AlaTyr: 1.906 ± 0.063
0.0AlaXaa: 0.0 ± 0.0
Cys
0.673CysAla: 0.673 ± 0.046
0.199CysCys: 0.199 ± 0.025
0.837CysAsp: 0.837 ± 0.043
0.656CysGlu: 0.656 ± 0.037
0.389CysPhe: 0.389 ± 0.03
1.299CysGly: 1.299 ± 0.078
0.241CysHis: 0.241 ± 0.026
0.958CysIle: 0.958 ± 0.047
0.687CysLys: 0.687 ± 0.043
0.798CysLeu: 0.798 ± 0.04
0.257CysMet: 0.257 ± 0.025
0.652CysAsn: 0.652 ± 0.046
0.679CysPro: 0.679 ± 0.057
0.284CysGln: 0.284 ± 0.026
0.371CysArg: 0.371 ± 0.032
0.709CysSer: 0.709 ± 0.045
0.592CysThr: 0.592 ± 0.038
0.885CysVal: 0.885 ± 0.051
0.075CysTrp: 0.075 ± 0.012
0.496CysTyr: 0.496 ± 0.036
0.0CysXaa: 0.0 ± 0.0
Asp
3.272AspAla: 3.272 ± 0.098
0.648AspCys: 0.648 ± 0.038
4.005AspAsp: 4.005 ± 0.114
4.975AspGlu: 4.975 ± 0.148
2.287AspPhe: 2.287 ± 0.08
3.199AspGly: 3.199 ± 0.1
0.717AspHis: 0.717 ± 0.043
6.594AspIle: 6.594 ± 0.131
5.15AspLys: 5.15 ± 0.124
5.022AspLeu: 5.022 ± 0.134
1.627AspMet: 1.627 ± 0.062
4.657AspAsn: 4.657 ± 0.142
1.491AspPro: 1.491 ± 0.055
0.829AspGln: 0.829 ± 0.042
1.436AspArg: 1.436 ± 0.052
3.326AspSer: 3.326 ± 0.07
3.665AspThr: 3.665 ± 0.111
4.692AspVal: 4.692 ± 0.094
0.369AspTrp: 0.369 ± 0.028
2.842AspTyr: 2.842 ± 0.076
0.0AspXaa: 0.0 ± 0.0
Glu
3.644GluAla: 3.644 ± 0.114
0.677GluCys: 0.677 ± 0.042
4.619GluAsp: 4.619 ± 0.133
5.618GluGlu: 5.618 ± 0.208
2.538GluPhe: 2.538 ± 0.091
3.312GluGly: 3.312 ± 0.088
1.155GluHis: 1.155 ± 0.063
6.906GluIle: 6.906 ± 0.161
6.321GluLys: 6.321 ± 0.153
5.79GluLeu: 5.79 ± 0.158
1.505GluMet: 1.505 ± 0.061
5.832GluAsn: 5.832 ± 0.131
1.371GluPro: 1.371 ± 0.057
1.538GluGln: 1.538 ± 0.072
1.918GluArg: 1.918 ± 0.078
3.505GluSer: 3.505 ± 0.099
3.503GluThr: 3.503 ± 0.095
4.493GluVal: 4.493 ± 0.123
0.35GluTrp: 0.35 ± 0.028
3.351GluTyr: 3.351 ± 0.096
0.0GluXaa: 0.0 ± 0.0
Phe
1.669PheAla: 1.669 ± 0.054
0.357PheCys: 0.357 ± 0.028
2.287PheAsp: 2.287 ± 0.073
2.35PheGlu: 2.35 ± 0.088
1.359PhePhe: 1.359 ± 0.06
2.311PheGly: 2.311 ± 0.083
0.594PheHis: 0.594 ± 0.04
3.837PheIle: 3.837 ± 0.117
3.108PheLys: 3.108 ± 0.089
2.95PheLeu: 2.95 ± 0.091
0.881PheMet: 0.881 ± 0.039
3.027PheAsn: 3.027 ± 0.099
0.976PhePro: 0.976 ± 0.05
0.774PheGln: 0.774 ± 0.049
1.163PheArg: 1.163 ± 0.047
2.212PheSer: 2.212 ± 0.078
2.265PheThr: 2.265 ± 0.079
1.939PheVal: 1.939 ± 0.063
0.176PheTrp: 0.176 ± 0.018
1.426PheTyr: 1.426 ± 0.06
0.0PheXaa: 0.0 ± 0.0
Gly
3.574GlyAla: 3.574 ± 0.133
0.95GlyCys: 0.95 ± 0.059
3.136GlyAsp: 3.136 ± 0.075
3.199GlyGlu: 3.199 ± 0.085
2.429GlyPhe: 2.429 ± 0.083
4.076GlyGly: 4.076 ± 0.13
1.141GlyHis: 1.141 ± 0.047
6.088GlyIle: 6.088 ± 0.114
4.463GlyLys: 4.463 ± 0.114
4.749GlyLeu: 4.749 ± 0.112
1.465GlyMet: 1.465 ± 0.06
3.906GlyAsn: 3.906 ± 0.209
1.453GlyPro: 1.453 ± 0.063
1.459GlyGln: 1.459 ± 0.06
1.995GlyArg: 1.995 ± 0.077
4.025GlySer: 4.025 ± 0.102
3.671GlyThr: 3.671 ± 0.116
4.457GlyVal: 4.457 ± 0.102
0.431GlyTrp: 0.431 ± 0.034
2.638GlyTyr: 2.638 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
0.938HisAla: 0.938 ± 0.048
0.239HisCys: 0.239 ± 0.025
1.074HisAsp: 1.074 ± 0.048
1.369HisGlu: 1.369 ± 0.055
0.494HisPhe: 0.494 ± 0.031
1.132HisGly: 1.132 ± 0.055
0.399HisHis: 0.399 ± 0.035
1.637HisIle: 1.637 ± 0.055
1.234HisLys: 1.234 ± 0.05
1.268HisLeu: 1.268 ± 0.061
0.48HisMet: 0.48 ± 0.034
1.106HisAsn: 1.106 ± 0.045
0.792HisPro: 0.792 ± 0.042
0.367HisGln: 0.367 ± 0.035
0.551HisArg: 0.551 ± 0.034
0.879HisSer: 0.879 ± 0.047
1.053HisThr: 1.053 ± 0.049
1.145HisVal: 1.145 ± 0.05
0.089HisTrp: 0.089 ± 0.013
0.693HisTyr: 0.693 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
5.245IleAla: 5.245 ± 0.137
1.24IleCys: 1.24 ± 0.054
6.339IleAsp: 6.339 ± 0.138
7.131IleGlu: 7.131 ± 0.148
3.561IlePhe: 3.561 ± 0.098
5.725IleGly: 5.725 ± 0.119
1.736IleHis: 1.736 ± 0.065
11.071IleIle: 11.071 ± 0.242
8.326IleLys: 8.326 ± 0.136
8.703IleLeu: 8.703 ± 0.174
2.17IleMet: 2.17 ± 0.076
7.635IleAsn: 7.635 ± 0.268
3.875IlePro: 3.875 ± 0.096
2.536IleGln: 2.536 ± 0.077
2.96IleArg: 2.96 ± 0.097
6.705IleSer: 6.705 ± 0.13
7.834IleThr: 7.834 ± 0.29
5.919IleVal: 5.919 ± 0.128
0.429IleTrp: 0.429 ± 0.032
3.999IleTyr: 3.999 ± 0.136
0.0IleXaa: 0.0 ± 0.0
Lys
3.786LysAla: 3.786 ± 0.099
0.835LysCys: 0.835 ± 0.051
4.907LysAsp: 4.907 ± 0.122
6.695LysGlu: 6.695 ± 0.161
2.283LysPhe: 2.283 ± 0.065
3.462LysGly: 3.462 ± 0.093
1.507LysHis: 1.507 ± 0.057
8.63LysIle: 8.63 ± 0.156
6.9LysLys: 6.9 ± 0.191
6.861LysLeu: 6.861 ± 0.143
1.671LysMet: 1.671 ± 0.064
6.31LysAsn: 6.31 ± 0.142
2.575LysPro: 2.575 ± 0.072
2.654LysGln: 2.654 ± 0.09
2.585LysArg: 2.585 ± 0.104
4.532LysSer: 4.532 ± 0.101
5.984LysThr: 5.984 ± 0.147
4.686LysVal: 4.686 ± 0.107
0.466LysTrp: 0.466 ± 0.031
4.272LysTyr: 4.272 ± 0.121
0.0LysXaa: 0.0 ± 0.0
Leu
4.309LeuAla: 4.309 ± 0.124
0.936LeuCys: 0.936 ± 0.047
5.391LeuAsp: 5.391 ± 0.133
5.966LeuGlu: 5.966 ± 0.156
3.337LeuPhe: 3.337 ± 0.111
4.755LeuGly: 4.755 ± 0.119
1.355LeuHis: 1.355 ± 0.053
7.643LeuIle: 7.643 ± 0.156
7.441LeuLys: 7.441 ± 0.152
7.327LeuLeu: 7.327 ± 0.176
1.878LeuMet: 1.878 ± 0.07
6.235LeuAsn: 6.235 ± 0.145
3.002LeuPro: 3.002 ± 0.087
1.89LeuGln: 1.89 ± 0.067
2.706LeuArg: 2.706 ± 0.087
5.877LeuSer: 5.877 ± 0.139
5.186LeuThr: 5.186 ± 0.127
4.771LeuVal: 4.771 ± 0.117
0.482LeuTrp: 0.482 ± 0.033
3.185LeuTyr: 3.185 ± 0.087
0.0LeuXaa: 0.0 ± 0.0
Met
1.428MetAla: 1.428 ± 0.056
0.286MetCys: 0.286 ± 0.025
1.592MetAsp: 1.592 ± 0.069
1.649MetGlu: 1.649 ± 0.074
0.788MetPhe: 0.788 ± 0.047
1.629MetGly: 1.629 ± 0.059
0.365MetHis: 0.365 ± 0.027
2.19MetIle: 2.19 ± 0.086
2.064MetLys: 2.064 ± 0.066
1.736MetLeu: 1.736 ± 0.064
0.486MetMet: 0.486 ± 0.032
1.56MetAsn: 1.56 ± 0.063
0.691MetPro: 0.691 ± 0.044
0.527MetGln: 0.527 ± 0.03
0.65MetArg: 0.65 ± 0.043
1.483MetSer: 1.483 ± 0.058
1.412MetThr: 1.412 ± 0.054
1.398MetVal: 1.398 ± 0.055
0.097MetTrp: 0.097 ± 0.014
0.719MetTyr: 0.719 ± 0.041
0.0MetXaa: 0.0 ± 0.0
Asn
3.031AsnAla: 3.031 ± 0.089
0.829AsnCys: 0.829 ± 0.053
3.533AsnAsp: 3.533 ± 0.098
4.062AsnGlu: 4.062 ± 0.121
2.141AsnPhe: 2.141 ± 0.081
4.185AsnGly: 4.185 ± 0.23
1.106AsnHis: 1.106 ± 0.046
9.013AsnIle: 9.013 ± 0.277
6.608AsnLys: 6.608 ± 0.145
5.368AsnLeu: 5.368 ± 0.13
1.91AsnMet: 1.91 ± 0.065
7.913AsnAsn: 7.913 ± 0.462
2.798AsnPro: 2.798 ± 0.075
2.172AsnGln: 2.172 ± 0.098
1.989AsnArg: 1.989 ± 0.06
4.611AsnSer: 4.611 ± 0.184
6.041AsnThr: 6.041 ± 0.352
4.07AsnVal: 4.07 ± 0.141
0.381AsnTrp: 0.381 ± 0.03
3.359AsnTyr: 3.359 ± 0.129
0.0AsnXaa: 0.0 ± 0.0
Pro
1.499ProAla: 1.499 ± 0.061
0.35ProCys: 0.35 ± 0.027
1.86ProAsp: 1.86 ± 0.068
2.858ProGlu: 2.858 ± 0.098
1.266ProPhe: 1.266 ± 0.05
1.995ProGly: 1.995 ± 0.069
0.642ProHis: 0.642 ± 0.037
2.984ProIle: 2.984 ± 0.073
2.131ProLys: 2.131 ± 0.079
2.794ProLeu: 2.794 ± 0.09
0.628ProMet: 0.628 ± 0.038
1.59ProAsn: 1.59 ± 0.059
0.772ProPro: 0.772 ± 0.043
0.999ProGln: 0.999 ± 0.042
1.023ProArg: 1.023 ± 0.048
2.176ProSer: 2.176 ± 0.076
2.135ProThr: 2.135 ± 0.065
2.277ProVal: 2.277 ± 0.074
0.229ProTrp: 0.229 ± 0.023
1.455ProTyr: 1.455 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
1.416GlnAla: 1.416 ± 0.057
0.239GlnCys: 0.239 ± 0.021
1.28GlnAsp: 1.28 ± 0.055
1.957GlnGlu: 1.957 ± 0.084
0.891GlnPhe: 0.891 ± 0.044
1.24GlnGly: 1.24 ± 0.052
0.454GlnHis: 0.454 ± 0.033
2.484GlnIle: 2.484 ± 0.081
2.368GlnLys: 2.368 ± 0.083
2.425GlnLeu: 2.425 ± 0.098
0.632GlnMet: 0.632 ± 0.039
1.554GlnAsn: 1.554 ± 0.066
0.778GlnPro: 0.778 ± 0.041
1.001GlnGln: 1.001 ± 0.055
0.879GlnArg: 0.879 ± 0.05
1.527GlnSer: 1.527 ± 0.064
1.718GlnThr: 1.718 ± 0.072
1.615GlnVal: 1.615 ± 0.059
0.164GlnTrp: 0.164 ± 0.02
1.236GlnTyr: 1.236 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
1.611ArgAla: 1.611 ± 0.063
0.355ArgCys: 0.355 ± 0.033
1.793ArgAsp: 1.793 ± 0.069
2.474ArgGlu: 2.474 ± 0.077
1.122ArgPhe: 1.122 ± 0.047
1.868ArgGly: 1.868 ± 0.071
0.476ArgHis: 0.476 ± 0.035
2.84ArgIle: 2.84 ± 0.089
2.625ArgLys: 2.625 ± 0.09
2.465ArgLeu: 2.465 ± 0.093
0.662ArgMet: 0.662 ± 0.037
2.234ArgAsn: 2.234 ± 0.068
0.942ArgPro: 0.942 ± 0.051
0.893ArgGln: 0.893 ± 0.049
1.266ArgArg: 1.266 ± 0.063
1.592ArgSer: 1.592 ± 0.063
1.631ArgThr: 1.631 ± 0.066
1.906ArgVal: 1.906 ± 0.063
0.178ArgTrp: 0.178 ± 0.02
1.299ArgTyr: 1.299 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
2.858SerAla: 2.858 ± 0.088
0.754SerCys: 0.754 ± 0.046
3.642SerAsp: 3.642 ± 0.09
3.533SerGlu: 3.533 ± 0.098
2.593SerPhe: 2.593 ± 0.091
4.068SerGly: 4.068 ± 0.099
1.031SerHis: 1.031 ± 0.045
6.454SerIle: 6.454 ± 0.122
5.261SerLys: 5.261 ± 0.118
5.093SerLeu: 5.093 ± 0.12
1.481SerMet: 1.481 ± 0.059
4.755SerAsn: 4.755 ± 0.169
1.787SerPro: 1.787 ± 0.071
1.856SerGln: 1.856 ± 0.076
1.977SerArg: 1.977 ± 0.065
4.562SerSer: 4.562 ± 0.146
4.204SerThr: 4.204 ± 0.14
3.709SerVal: 3.709 ± 0.093
0.423SerTrp: 0.423 ± 0.032
2.619SerTyr: 2.619 ± 0.087
0.0SerXaa: 0.0 ± 0.0
Thr
3.231ThrAla: 3.231 ± 0.104
0.703ThrCys: 0.703 ± 0.038
3.73ThrAsp: 3.73 ± 0.106
3.341ThrGlu: 3.341 ± 0.082
2.336ThrPhe: 2.336 ± 0.095
4.299ThrGly: 4.299 ± 0.137
1.175ThrHis: 1.175 ± 0.051
7.795ThrIle: 7.795 ± 0.344
4.73ThrLys: 4.73 ± 0.117
6.209ThrLeu: 6.209 ± 0.162
1.191ThrMet: 1.191 ± 0.052
4.674ThrAsn: 4.674 ± 0.248
2.668ThrPro: 2.668 ± 0.075
1.937ThrGln: 1.937 ± 0.069
1.803ThrArg: 1.803 ± 0.066
4.353ThrSer: 4.353 ± 0.126
5.53ThrThr: 5.53 ± 0.294
4.412ThrVal: 4.412 ± 0.18
0.322ThrTrp: 0.322 ± 0.029
2.962ThrTyr: 2.962 ± 0.098
0.0ThrXaa: 0.0 ± 0.0
Val
3.758ValAla: 3.758 ± 0.101
0.849ValCys: 0.849 ± 0.04
4.465ValAsp: 4.465 ± 0.095
4.347ValGlu: 4.347 ± 0.11
2.275ValPhe: 2.275 ± 0.074
3.981ValGly: 3.981 ± 0.119
0.932ValHis: 0.932 ± 0.046
6.065ValIle: 6.065 ± 0.124
4.588ValLys: 4.588 ± 0.105
5.413ValLeu: 5.413 ± 0.111
1.317ValMet: 1.317 ± 0.05
4.276ValAsn: 4.276 ± 0.149
1.971ValPro: 1.971 ± 0.072
1.331ValGln: 1.331 ± 0.056
1.785ValArg: 1.785 ± 0.078
4.297ValSer: 4.297 ± 0.112
4.123ValThr: 4.123 ± 0.157
4.374ValVal: 4.374 ± 0.119
0.334ValTrp: 0.334 ± 0.024
2.544ValTyr: 2.544 ± 0.074
0.0ValXaa: 0.0 ± 0.0
Trp
0.286TrpAla: 0.286 ± 0.021
0.047TrpCys: 0.047 ± 0.011
0.369TrpAsp: 0.369 ± 0.028
0.273TrpGlu: 0.273 ± 0.026
0.237TrpPhe: 0.237 ± 0.023
0.393TrpGly: 0.393 ± 0.029
0.107TrpHis: 0.107 ± 0.016
0.557TrpIle: 0.557 ± 0.039
0.413TrpLys: 0.413 ± 0.032
0.436TrpLeu: 0.436 ± 0.029
0.172TrpMet: 0.172 ± 0.02
0.474TrpAsn: 0.474 ± 0.038
0.16TrpPro: 0.16 ± 0.02
0.144TrpGln: 0.144 ± 0.02
0.166TrpArg: 0.166 ± 0.02
0.296TrpSer: 0.296 ± 0.023
0.363TrpThr: 0.363 ± 0.028
0.284TrpVal: 0.284 ± 0.023
0.071TrpTrp: 0.071 ± 0.013
0.278TrpTyr: 0.278 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.222TyrAla: 2.222 ± 0.075
0.533TyrCys: 0.533 ± 0.037
3.146TyrAsp: 3.146 ± 0.094
2.753TyrGlu: 2.753 ± 0.084
1.517TyrPhe: 1.517 ± 0.056
2.897TyrGly: 2.897 ± 0.084
0.774TyrHis: 0.774 ± 0.038
3.904TyrIle: 3.904 ± 0.111
3.116TyrLys: 3.116 ± 0.092
3.482TyrLeu: 3.482 ± 0.095
1.019TyrMet: 1.019 ± 0.045
3.831TyrAsn: 3.831 ± 0.16
1.461TyrPro: 1.461 ± 0.059
0.999TyrGln: 0.999 ± 0.048
1.112TyrArg: 1.112 ± 0.056
2.654TyrSer: 2.654 ± 0.077
3.02TyrThr: 3.02 ± 0.153
2.625TyrVal: 2.625 ± 0.069
0.213TyrTrp: 0.213 ± 0.021
2.012TyrTyr: 2.012 ± 0.075
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1533 proteins (493630 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski