Streptomyces turgidiscabies Car8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces turgidiscabies

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7FED3|L7FED3_9ACTN Histidine kinase OS=Streptomyces turgidiscabies Car8 OX=698760 GN=STRTUCAR8_09237 PE=4 SV=1
MM1 pKa = 7.4IPVPKK6 pKa = 10.3VFYY9 pKa = 7.98TQPDD13 pKa = 3.52GSEE16 pKa = 4.16TVVEE20 pKa = 4.52AVAGDD25 pKa = 3.98SVMQTAVRR33 pKa = 11.84NAVSGILGQCGGSLSCATCHH53 pKa = 6.23VFLAEE58 pKa = 4.17EE59 pKa = 4.22NTDD62 pKa = 3.47DD63 pKa = 4.07FAPVSEE69 pKa = 5.64DD70 pKa = 3.35EE71 pKa = 6.75DD72 pKa = 4.23EE73 pKa = 4.64MLDD76 pKa = 3.63CAATEE81 pKa = 4.45RR82 pKa = 11.84EE83 pKa = 4.07DD84 pKa = 4.4SSRR87 pKa = 11.84LSCQLVLGDD96 pKa = 3.76GQEE99 pKa = 4.01VHH101 pKa = 5.17VTVPEE106 pKa = 4.13AQLL109 pKa = 3.44

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7EYW6|L7EYW6_9ACTN Urease accessory protein UreG OS=Streptomyces turgidiscabies Car8 OX=698760 GN=ureG PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10034

0

10034

3140125

14

6254

312.9

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.081 ± 0.038

0.8 ± 0.007

6.056 ± 0.021

5.568 ± 0.026

2.73 ± 0.016

9.409 ± 0.026

2.294 ± 0.013

3.184 ± 0.018

2.27 ± 0.022

10.07 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.754 ± 0.01

1.948 ± 0.016

6.026 ± 0.025

2.874 ± 0.015

7.894 ± 0.03

5.414 ± 0.021

6.492 ± 0.025

8.392 ± 0.021

1.572 ± 0.012

2.172 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski