Sphaerulina musiva (strain SO2202) (Poplar stem canker fungus) (Septoria musiva)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Sphaerulina; Sphaerulina musiva

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M3AXA3|M3AXA3_SPHMS Uncharacterized protein OS=Sphaerulina musiva (strain SO2202) OX=692275 GN=SEPMUDRAFT_164868 PE=3 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84QADD5 pKa = 3.99GVAAASLLLLLLPCILAQQITSTISIPYY33 pKa = 9.55CSTSPGLFTIYY44 pKa = 10.91GSMTSAIPSNILSSNSQTPTAGLLSSSVSSVATPLASAYY83 pKa = 9.97VDD85 pKa = 3.52QYY87 pKa = 10.23GGKK90 pKa = 8.54YY91 pKa = 7.57TVQFDD96 pKa = 3.57TGLVGSTIPASRR108 pKa = 11.84IDD110 pKa = 3.7GLRR113 pKa = 11.84KK114 pKa = 9.51RR115 pKa = 11.84QAIDD119 pKa = 3.51IGTLDD124 pKa = 3.62QTFEE128 pKa = 4.56DD129 pKa = 4.49CLEE132 pKa = 4.37SCTLTSACVAVAALDD147 pKa = 3.83STCQLFSSVRR157 pKa = 11.84EE158 pKa = 4.11TVSLVGGKK166 pKa = 8.99VAFNQEE172 pKa = 3.67RR173 pKa = 11.84FLGFSNGTILPSSMATEE190 pKa = 4.41ASSAQGMASASAISSGGFRR209 pKa = 11.84SSMPGLASMPGGSMTPLSQLPSTLAMEE236 pKa = 4.85TSTAGPLVSSFVTQDD251 pKa = 2.88ISPAGSSSDD260 pKa = 3.28IMPSAALSTNTVTTILSSGAVLSATSEE287 pKa = 4.12PLSTFSSAGDD297 pKa = 3.52PTITLQVPSSLSTEE311 pKa = 4.08SSVSISSTSSTPTDD325 pKa = 3.38QLVSTMTSDD334 pKa = 4.49GEE336 pKa = 4.07ISYY339 pKa = 8.31STDD342 pKa = 3.03LTITSSLSSLATPASASISSAASSSLALSSVASVSSSRR380 pKa = 11.84SRR382 pKa = 11.84AEE384 pKa = 3.92SSSSSSSPGPINASTFFSSPAVPTTTRR411 pKa = 11.84TTTTTTSSSSSSSSPATPTSSRR433 pKa = 11.84KK434 pKa = 9.52PPTCPDD440 pKa = 4.17DD441 pKa = 5.54DD442 pKa = 6.04GEE444 pKa = 5.67DD445 pKa = 3.85YY446 pKa = 11.1IDD448 pKa = 3.6TNGFIYY454 pKa = 10.21IITCDD459 pKa = 2.88IGFNDD464 pKa = 3.94EE465 pKa = 4.6TIQTTTVQNFVDD477 pKa = 5.98CITTCDD483 pKa = 4.27AFNEE487 pKa = 4.16LPKK490 pKa = 10.72DD491 pKa = 3.64DD492 pKa = 4.83EE493 pKa = 4.47EE494 pKa = 5.26VMGCGGVNFNSLAQTDD510 pKa = 4.03NCLLMPGAEE519 pKa = 4.02IAEE522 pKa = 4.29MAGIWSAQLSGSIAEE537 pKa = 4.24

Molecular weight: 54.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N1QH86|N1QH86_SPHMS Mitochondrial ribosomal protein S19 OS=Sphaerulina musiva (strain SO2202) OX=692275 GN=SEPMUDRAFT_72028 PE=3 SV=1
RR1 pKa = 7.12GNSRR5 pKa = 11.84IGGNSTPNRR14 pKa = 11.84NGRR17 pKa = 11.84ISGNSTPNGNSRR29 pKa = 11.84IGGNSTPNRR38 pKa = 11.84NGRR41 pKa = 11.84ISGNSTPNGNSRR53 pKa = 11.84IGGNSS58 pKa = 2.85

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10154

0

10154

4580991

49

4947

451.2

49.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.089 ± 0.021

1.203 ± 0.009

5.72 ± 0.018

6.216 ± 0.021

3.472 ± 0.015

6.968 ± 0.021

2.576 ± 0.01

4.621 ± 0.015

4.849 ± 0.022

8.613 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.009

3.585 ± 0.015

5.949 ± 0.028

4.342 ± 0.018

6.184 ± 0.025

8.145 ± 0.031

6.113 ± 0.018

5.971 ± 0.016

1.411 ± 0.01

2.726 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski