Thermoplasmatales archaeon I-plasma

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales; unclassified Thermoplasmatales

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0LAW9|T0LAW9_9ARCH DLH domain-containing protein OS=Thermoplasmatales archaeon I-plasma OX=667138 GN=AMDU3_IPLC00004G0540 PE=4 SV=1
MM1 pKa = 7.49NNRR4 pKa = 11.84LKK6 pKa = 10.52IVIAVVVVLAVVVGGFVIYY25 pKa = 10.8HH26 pKa = 6.7EE27 pKa = 4.98DD28 pKa = 3.31ATGKK32 pKa = 10.59AKK34 pKa = 11.02GEE36 pKa = 4.3IITITTGDD44 pKa = 4.02NIPYY48 pKa = 9.58KK49 pKa = 9.63LTRR52 pKa = 11.84IVSLDD57 pKa = 3.42PAATATIYY65 pKa = 11.39ALGAYY70 pKa = 9.57NDD72 pKa = 4.07LVGGNSYY79 pKa = 11.41DD80 pKa = 3.65FYY82 pKa = 10.9PPNEE86 pKa = 4.04NLPNVTDD93 pKa = 3.98YY94 pKa = 11.57PSMDD98 pKa = 3.53LEE100 pKa = 4.71QIFNLSADD108 pKa = 3.62AVISFSNYY116 pKa = 9.38SGAQISQLLNSGINYY131 pKa = 9.13VFLSSDD137 pKa = 2.93AGVNFSVIEE146 pKa = 4.06KK147 pKa = 10.55QNTLLGEE154 pKa = 4.26LTGTVANATLINQWMNVSLSTLHH177 pKa = 7.09EE178 pKa = 4.71DD179 pKa = 3.63ALSYY183 pKa = 10.74DD184 pKa = 3.2QSEE187 pKa = 4.82YY188 pKa = 11.29NGTPLRR194 pKa = 11.84GFYY197 pKa = 10.53YY198 pKa = 10.19LSSYY202 pKa = 9.44GGYY205 pKa = 6.6WTAGNDD211 pKa = 3.56TFVNSYY217 pKa = 9.26FQYY220 pKa = 11.62ADD222 pKa = 3.92IINIAAPYY230 pKa = 10.44DD231 pKa = 3.55SGFYY235 pKa = 10.07TINPEE240 pKa = 4.21NIVNEE245 pKa = 4.2SPQIVILDD253 pKa = 3.72DD254 pKa = 4.54SINASSLNATGSPFASSPAVMNGKK278 pKa = 9.99VYY280 pKa = 7.61TTTSNEE286 pKa = 4.08YY287 pKa = 10.02MFTEE291 pKa = 4.43PNFRR295 pKa = 11.84DD296 pKa = 2.97IFAIQWIIFMVYY308 pKa = 9.37GQNPALPSFPINLTYY323 pKa = 10.86NPNPNGVSS331 pKa = 3.2

Molecular weight: 36.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0LDE3|T0LDE3_9ARCH Thioesterase superfamily protein OS=Thermoplasmatales archaeon I-plasma OX=667138 GN=AMDU3_IPLC00001G0043 PE=3 SV=1
MM1 pKa = 7.52SYY3 pKa = 10.52HH4 pKa = 5.3YY5 pKa = 10.51VRR7 pKa = 11.84NILRR11 pKa = 11.84QIGARR16 pKa = 11.84GGVPRR21 pKa = 11.84FHH23 pKa = 6.91AHH25 pKa = 7.64AARR28 pKa = 11.84LWCATALLRR37 pKa = 11.84GIFGQKK43 pKa = 9.8PLDD46 pKa = 3.29IRR48 pKa = 11.84MVQIHH53 pKa = 7.43LDD55 pKa = 3.41HH56 pKa = 7.19KK57 pKa = 10.96SPKK60 pKa = 6.78TTQRR64 pKa = 11.84YY65 pKa = 4.94THH67 pKa = 5.39VCRR70 pKa = 11.84LEE72 pKa = 4.04VSDD75 pKa = 4.4

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1693

0

1693

492143

31

2927

290.7

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.203 ± 0.051

0.578 ± 0.016

5.038 ± 0.055

6.694 ± 0.08

4.731 ± 0.04

7.608 ± 0.052

1.461 ± 0.021

8.132 ± 0.05

6.746 ± 0.077

9.101 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.031

4.48 ± 0.05

3.886 ± 0.039

2.282 ± 0.032

5.1 ± 0.063

7.821 ± 0.103

5.207 ± 0.099

7.371 ± 0.052

0.917 ± 0.021

3.941 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski