Lactobacillus psittaci DSM 15354
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1327 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1S2T2|A0A0R1S2T2_9LACO PpGpp synthetase hydrolase catalytic subunit OS=Lactobacillus psittaci DSM 15354 OX=1122152 GN=FC23_GL000947 PE=4 SV=1
MM1 pKa = 7.66 EE2 pKa = 6.16 DD3 pKa = 3.5 KK4 pKa = 10.97 SITLTTDD11 pKa = 2.94 YY12 pKa = 9.59 ATNSINMNFSDD23 pKa = 4.61 NLTDD27 pKa = 3.84 GQEE30 pKa = 3.44 IGYY33 pKa = 9.29 ILSASFLSYY42 pKa = 10.73 AINQGLSKK50 pKa = 11.11 EE51 pKa = 4.4 EE52 pKa = 3.78 IVKK55 pKa = 10.35 LVSEE59 pKa = 5.4 GYY61 pKa = 10.7 DD62 pKa = 3.24 EE63 pKa = 4.42 FTSDD67 pKa = 4.09 NNN69 pKa = 3.66
Molecular weight: 7.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.77
IPC_protein 3.656
Toseland 3.465
ProMoST 3.846
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.567
Nozaki 3.795
DTASelect 3.948
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.783
Patrickios 1.837
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A0R1S474|A0A0R1S474_9LACO Peptide chain release factor N(5)-glutamine methyltransferase OS=Lactobacillus psittaci DSM 15354 OX=1122152 GN=FC23_GL000674 PE=4 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.14 QKK5 pKa = 7.59 THH7 pKa = 6.46 RR8 pKa = 11.84 ASAKK12 pKa = 9.12 RR13 pKa = 11.84 FKK15 pKa = 10.26 RR16 pKa = 11.84 TGNGGLKK23 pKa = 9.42 RR24 pKa = 11.84 HH25 pKa = 6.17 HH26 pKa = 7.23 AFTGHH31 pKa = 6.77 RR32 pKa = 11.84 FHH34 pKa = 7.54 GKK36 pKa = 6.24 TKK38 pKa = 9.47 KK39 pKa = 9.37 QRR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.45 LRR45 pKa = 11.84 KK46 pKa = 8.75 AAMVSRR52 pKa = 11.84 SDD54 pKa = 3.67 LKK56 pKa = 10.76 RR57 pKa = 11.84 IKK59 pKa = 10.66 QMLSQMRR66 pKa = 3.59
Molecular weight: 7.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.43
IPC2_protein 10.935
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.501
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.676
DTASelect 12.661
Thurlkill 12.676
EMBOSS 13.159
Sillero 12.676
Patrickios 12.223
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.037
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1327
0
1327
455147
38
12394
343.0
38.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.721 ± 0.094
0.519 ± 0.022
5.82 ± 0.14
5.732 ± 0.097
4.474 ± 0.094
6.416 ± 0.067
1.868 ± 0.031
7.433 ± 0.091
7.901 ± 0.076
9.767 ± 0.219
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.443 ± 0.066
5.341 ± 0.053
3.396 ± 0.059
3.934 ± 0.044
3.484 ± 0.076
6.302 ± 0.059
5.971 ± 0.273
6.832 ± 0.109
0.931 ± 0.025
3.714 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here