Nitrosomonas sp. Nm143

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; unclassified Nitrosomonas

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328ZZM7|A0A328ZZM7_9PROT Glycosyl transferase family 2 OS=Nitrosomonas sp. Nm143 OX=200123 GN=C8R33_11133 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84NYY4 pKa = 10.82NEE6 pKa = 3.66WEE8 pKa = 3.88LRR10 pKa = 11.84KK11 pKa = 9.7LQLEE15 pKa = 4.25LQQLDD20 pKa = 3.57EE21 pKa = 5.77DD22 pKa = 4.28FTEE25 pKa = 4.4LNHH28 pKa = 5.94YY29 pKa = 9.38FNSEE33 pKa = 3.57SFYY36 pKa = 10.95FSNEE40 pKa = 3.86DD41 pKa = 3.3FDD43 pKa = 5.64EE44 pKa = 4.42STTDD48 pKa = 3.14IMVEE52 pKa = 3.95NSHH55 pKa = 7.23DD56 pKa = 4.09ADD58 pKa = 5.01FNDD61 pKa = 3.58QSIALFTSIDD71 pKa = 3.74ATDD74 pKa = 3.6EE75 pKa = 4.35EE76 pKa = 5.54YY77 pKa = 10.89DD78 pKa = 3.61VQMLLEE84 pKa = 4.29GKK86 pKa = 10.19YY87 pKa = 10.72NYY89 pKa = 10.84

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328ZZB0|A0A328ZZB0_9PROT Twitching motility protein PilU OS=Nitrosomonas sp. Nm143 OX=200123 GN=C8R33_12643 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.6QPSVIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.64RR14 pKa = 11.84THH16 pKa = 6.0GFLVRR21 pKa = 11.84MKK23 pKa = 8.97TRR25 pKa = 11.84SGAAVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84ARR41 pKa = 11.84LGVNN45 pKa = 3.56

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3249

0

3249

1053642

27

3816

324.3

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.659 ± 0.065

1.004 ± 0.012

5.441 ± 0.032

5.871 ± 0.047

4.137 ± 0.032

6.698 ± 0.045

2.482 ± 0.024

7.16 ± 0.037

5.011 ± 0.048

10.488 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.025

4.528 ± 0.047

4.282 ± 0.032

4.248 ± 0.037

5.315 ± 0.037

6.107 ± 0.044

5.456 ± 0.055

6.478 ± 0.036

1.273 ± 0.017

2.94 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski