Amino acid dipepetide frequency for Nitrosomonas sp. Nm143

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.592AlaAla: 8.592 ± 0.172
0.957AlaCys: 0.957 ± 0.031
4.669AlaAsp: 4.669 ± 0.094
5.18AlaGlu: 5.18 ± 0.084
3.289AlaPhe: 3.289 ± 0.066
6.205AlaGly: 6.205 ± 0.114
1.96AlaHis: 1.96 ± 0.048
6.265AlaIle: 6.265 ± 0.088
4.129AlaLys: 4.129 ± 0.071
9.494AlaLeu: 9.494 ± 0.105
2.256AlaMet: 2.256 ± 0.05
3.624AlaAsn: 3.624 ± 0.084
2.935AlaPro: 2.935 ± 0.058
3.606AlaGln: 3.606 ± 0.072
4.459AlaArg: 4.459 ± 0.071
4.999AlaSer: 4.999 ± 0.092
4.574AlaThr: 4.574 ± 0.116
5.832AlaVal: 5.832 ± 0.091
1.08AlaTrp: 1.08 ± 0.036
2.485AlaTyr: 2.485 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.884CysAla: 0.884 ± 0.031
0.149CysCys: 0.149 ± 0.013
0.52CysAsp: 0.52 ± 0.023
0.539CysGlu: 0.539 ± 0.023
0.48CysPhe: 0.48 ± 0.022
0.894CysGly: 0.894 ± 0.03
0.361CysHis: 0.361 ± 0.022
0.646CysIle: 0.646 ± 0.026
0.4CysLys: 0.4 ± 0.019
0.965CysLeu: 0.965 ± 0.029
0.206CysMet: 0.206 ± 0.015
0.419CysAsn: 0.419 ± 0.019
0.476CysPro: 0.476 ± 0.024
0.405CysGln: 0.405 ± 0.02
0.539CysArg: 0.539 ± 0.023
0.573CysSer: 0.573 ± 0.023
0.449CysThr: 0.449 ± 0.021
0.651CysVal: 0.651 ± 0.027
0.139CysTrp: 0.139 ± 0.011
0.341CysTyr: 0.341 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.616AspAla: 4.616 ± 0.085
0.55AspCys: 0.55 ± 0.023
2.932AspAsp: 2.932 ± 0.071
3.552AspGlu: 3.552 ± 0.061
2.486AspPhe: 2.486 ± 0.047
3.633AspGly: 3.633 ± 0.078
1.346AspHis: 1.346 ± 0.039
3.997AspIle: 3.997 ± 0.069
2.743AspLys: 2.743 ± 0.053
5.368AspLeu: 5.368 ± 0.083
1.279AspMet: 1.279 ± 0.036
2.478AspAsn: 2.478 ± 0.051
2.443AspPro: 2.443 ± 0.05
2.111AspGln: 2.111 ± 0.057
2.682AspArg: 2.682 ± 0.062
2.983AspSer: 2.983 ± 0.06
2.856AspThr: 2.856 ± 0.091
3.584AspVal: 3.584 ± 0.076
0.867AspTrp: 0.867 ± 0.033
1.904AspTyr: 1.904 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
5.032GluAla: 5.032 ± 0.081
0.483GluCys: 0.483 ± 0.022
2.705GluAsp: 2.705 ± 0.058
3.498GluGlu: 3.498 ± 0.07
2.179GluPhe: 2.179 ± 0.051
3.073GluGly: 3.073 ± 0.06
1.316GluHis: 1.316 ± 0.041
4.982GluIle: 4.982 ± 0.077
3.936GluLys: 3.936 ± 0.081
5.985GluLeu: 5.985 ± 0.084
1.642GluMet: 1.642 ± 0.045
3.008GluAsn: 3.008 ± 0.054
2.045GluPro: 2.045 ± 0.046
2.897GluGln: 2.897 ± 0.054
3.365GluArg: 3.365 ± 0.065
3.384GluSer: 3.384 ± 0.061
3.46GluThr: 3.46 ± 0.06
3.369GluVal: 3.369 ± 0.075
0.794GluTrp: 0.794 ± 0.033
1.552GluTyr: 1.552 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.349PheAla: 3.349 ± 0.062
0.5PheCys: 0.5 ± 0.023
2.715PheAsp: 2.715 ± 0.053
2.335PheGlu: 2.335 ± 0.049
2.02PhePhe: 2.02 ± 0.053
2.987PheGly: 2.987 ± 0.066
0.949PheHis: 0.949 ± 0.03
2.942PheIle: 2.942 ± 0.06
1.766PheLys: 1.766 ± 0.044
3.949PheLeu: 3.949 ± 0.072
0.91PheMet: 0.91 ± 0.032
2.0PheAsn: 2.0 ± 0.05
1.74PhePro: 1.74 ± 0.044
1.389PheGln: 1.389 ± 0.038
1.888PheArg: 1.888 ± 0.043
3.175PheSer: 3.175 ± 0.062
2.183PheThr: 2.183 ± 0.055
2.644PheVal: 2.644 ± 0.053
0.528PheTrp: 0.528 ± 0.028
1.399PheTyr: 1.399 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
5.189GlyAla: 5.189 ± 0.118
0.804GlyCys: 0.804 ± 0.031
3.466GlyAsp: 3.466 ± 0.066
3.904GlyGlu: 3.904 ± 0.057
3.035GlyPhe: 3.035 ± 0.063
4.964GlyGly: 4.964 ± 0.094
1.707GlyHis: 1.707 ± 0.035
5.297GlyIle: 5.297 ± 0.087
3.883GlyLys: 3.883 ± 0.061
6.723GlyLeu: 6.723 ± 0.099
1.847GlyMet: 1.847 ± 0.042
3.067GlyAsn: 3.067 ± 0.086
1.844GlyPro: 1.844 ± 0.049
2.364GlyGln: 2.364 ± 0.047
3.478GlyArg: 3.478 ± 0.068
3.862GlySer: 3.862 ± 0.083
3.399GlyThr: 3.399 ± 0.076
4.747GlyVal: 4.747 ± 0.067
1.008GlyTrp: 1.008 ± 0.037
2.387GlyTyr: 2.387 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
2.164HisAla: 2.164 ± 0.05
0.346HisCys: 0.346 ± 0.019
1.355HisAsp: 1.355 ± 0.036
1.411HisGlu: 1.411 ± 0.036
1.077HisPhe: 1.077 ± 0.032
1.735HisGly: 1.735 ± 0.042
0.915HisHis: 0.915 ± 0.034
1.615HisIle: 1.615 ± 0.04
0.967HisLys: 0.967 ± 0.029
2.545HisLeu: 2.545 ± 0.058
0.534HisMet: 0.534 ± 0.021
0.969HisAsn: 0.969 ± 0.032
1.325HisPro: 1.325 ± 0.037
1.128HisGln: 1.128 ± 0.037
1.361HisArg: 1.361 ± 0.037
1.486HisSer: 1.486 ± 0.044
1.179HisThr: 1.179 ± 0.035
1.438HisVal: 1.438 ± 0.041
0.376HisTrp: 0.376 ± 0.019
0.893HisTyr: 0.893 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.771IleAla: 6.771 ± 0.097
0.752IleCys: 0.752 ± 0.028
4.495IleAsp: 4.495 ± 0.062
4.67IleGlu: 4.67 ± 0.071
2.796IlePhe: 2.796 ± 0.062
4.996IleGly: 4.996 ± 0.087
1.623IleHis: 1.623 ± 0.038
4.756IleIle: 4.756 ± 0.076
3.657IleLys: 3.657 ± 0.061
6.66IleLeu: 6.66 ± 0.093
1.4IleMet: 1.4 ± 0.04
3.572IleAsn: 3.572 ± 0.066
3.205IlePro: 3.205 ± 0.064
2.511IleGln: 2.511 ± 0.056
3.531IleArg: 3.531 ± 0.058
4.726IleSer: 4.726 ± 0.084
4.321IleThr: 4.321 ± 0.081
4.481IleVal: 4.481 ± 0.067
0.76IleTrp: 0.76 ± 0.029
1.917IleTyr: 1.917 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
3.796LysAla: 3.796 ± 0.069
0.38LysCys: 0.38 ± 0.018
2.402LysAsp: 2.402 ± 0.053
2.949LysGlu: 2.949 ± 0.059
1.64LysPhe: 1.64 ± 0.041
2.634LysGly: 2.634 ± 0.05
1.289LysHis: 1.289 ± 0.042
3.897LysIle: 3.897 ± 0.069
3.21LysLys: 3.21 ± 0.069
5.325LysLeu: 5.325 ± 0.081
1.294LysMet: 1.294 ± 0.036
2.772LysAsn: 2.772 ± 0.056
2.408LysPro: 2.408 ± 0.053
2.577LysGln: 2.577 ± 0.061
2.916LysArg: 2.916 ± 0.056
3.009LysSer: 3.009 ± 0.07
2.931LysThr: 2.931 ± 0.055
2.828LysVal: 2.828 ± 0.06
0.548LysTrp: 0.548 ± 0.025
1.305LysTyr: 1.305 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
9.73LeuAla: 9.73 ± 0.118
1.0LeuCys: 1.0 ± 0.031
5.496LeuAsp: 5.496 ± 0.078
5.732LeuGlu: 5.732 ± 0.081
4.414LeuPhe: 4.414 ± 0.096
6.564LeuGly: 6.564 ± 0.105
2.491LeuHis: 2.491 ± 0.051
7.181LeuIle: 7.181 ± 0.103
5.216LeuLys: 5.216 ± 0.084
11.228LeuLeu: 11.228 ± 0.149
2.501LeuMet: 2.501 ± 0.054
4.586LeuAsn: 4.586 ± 0.07
5.246LeuPro: 5.246 ± 0.089
4.271LeuGln: 4.271 ± 0.07
5.595LeuArg: 5.595 ± 0.078
7.34LeuSer: 7.34 ± 0.134
6.136LeuThr: 6.136 ± 0.082
6.338LeuVal: 6.338 ± 0.092
1.186LeuTrp: 1.186 ± 0.038
2.625LeuTyr: 2.625 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.143MetAla: 2.143 ± 0.05
0.167MetCys: 0.167 ± 0.014
1.122MetAsp: 1.122 ± 0.035
1.25MetGlu: 1.25 ± 0.036
0.794MetPhe: 0.794 ± 0.031
1.503MetGly: 1.503 ± 0.04
0.62MetHis: 0.62 ± 0.024
1.594MetIle: 1.594 ± 0.039
1.36MetLys: 1.36 ± 0.037
2.801MetLeu: 2.801 ± 0.059
0.66MetMet: 0.66 ± 0.024
1.139MetAsn: 1.139 ± 0.036
1.21MetPro: 1.21 ± 0.039
1.163MetGln: 1.163 ± 0.034
1.443MetArg: 1.443 ± 0.038
1.539MetSer: 1.539 ± 0.036
1.5MetThr: 1.5 ± 0.037
1.597MetVal: 1.597 ± 0.038
0.173MetTrp: 0.173 ± 0.013
0.457MetTyr: 0.457 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.866AsnAla: 3.866 ± 0.068
0.436AsnCys: 0.436 ± 0.02
2.534AsnAsp: 2.534 ± 0.07
2.648AsnGlu: 2.648 ± 0.054
1.877AsnPhe: 1.877 ± 0.049
3.073AsnGly: 3.073 ± 0.069
1.161AsnHis: 1.161 ± 0.04
3.252AsnIle: 3.252 ± 0.065
2.378AsnLys: 2.378 ± 0.06
4.333AsnLeu: 4.333 ± 0.079
1.012AsnMet: 1.012 ± 0.029
2.293AsnAsn: 2.293 ± 0.053
2.295AsnPro: 2.295 ± 0.05
2.09AsnGln: 2.09 ± 0.045
2.399AsnArg: 2.399 ± 0.051
2.465AsnSer: 2.465 ± 0.063
2.378AsnThr: 2.378 ± 0.063
2.717AsnVal: 2.717 ± 0.065
0.606AsnTrp: 0.606 ± 0.026
1.471AsnTyr: 1.471 ± 0.045
0.001AsnXaa: 0.001 ± 0.001
Pro
3.45ProAla: 3.45 ± 0.063
0.367ProCys: 0.367 ± 0.018
2.992ProAsp: 2.992 ± 0.058
3.111ProGlu: 3.111 ± 0.06
1.912ProPhe: 1.912 ± 0.043
3.102ProGly: 3.102 ± 0.056
0.947ProHis: 0.947 ± 0.033
2.881ProIle: 2.881 ± 0.061
1.849ProLys: 1.849 ± 0.045
4.352ProLeu: 4.352 ± 0.077
0.973ProMet: 0.973 ± 0.031
1.8ProAsn: 1.8 ± 0.052
1.76ProPro: 1.76 ± 0.05
1.687ProGln: 1.687 ± 0.037
1.829ProArg: 1.829 ± 0.047
2.446ProSer: 2.446 ± 0.046
2.187ProThr: 2.187 ± 0.053
3.382ProVal: 3.382 ± 0.063
0.54ProTrp: 0.54 ± 0.022
1.257ProTyr: 1.257 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
3.835GlnAla: 3.835 ± 0.083
0.384GlnCys: 0.384 ± 0.019
1.826GlnAsp: 1.826 ± 0.047
2.314GlnGlu: 2.314 ± 0.056
1.597GlnPhe: 1.597 ± 0.041
2.532GlnGly: 2.532 ± 0.05
1.183GlnHis: 1.183 ± 0.036
2.931GlnIle: 2.931 ± 0.064
2.114GlnLys: 2.114 ± 0.049
4.744GlnLeu: 4.744 ± 0.086
0.997GlnMet: 0.997 ± 0.03
1.65GlnAsn: 1.65 ± 0.043
1.918GlnPro: 1.918 ± 0.049
2.574GlnGln: 2.574 ± 0.056
2.608GlnArg: 2.608 ± 0.047
2.534GlnSer: 2.534 ± 0.056
2.237GlnThr: 2.237 ± 0.052
2.76GlnVal: 2.76 ± 0.061
0.596GlnTrp: 0.596 ± 0.026
1.145GlnTyr: 1.145 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
3.949ArgAla: 3.949 ± 0.067
0.5ArgCys: 0.5 ± 0.026
2.777ArgAsp: 2.777 ± 0.058
3.31ArgGlu: 3.31 ± 0.068
2.539ArgPhe: 2.539 ± 0.058
2.959ArgGly: 2.959 ± 0.056
1.538ArgHis: 1.538 ± 0.04
4.112ArgIle: 4.112 ± 0.059
2.699ArgLys: 2.699 ± 0.051
5.996ArgLeu: 5.996 ± 0.088
1.424ArgMet: 1.424 ± 0.039
2.379ArgAsn: 2.379 ± 0.044
1.991ArgPro: 1.991 ± 0.049
2.484ArgGln: 2.484 ± 0.061
3.144ArgArg: 3.144 ± 0.064
2.895ArgSer: 2.895 ± 0.057
2.423ArgThr: 2.423 ± 0.051
3.266ArgVal: 3.266 ± 0.052
0.755ArgTrp: 0.755 ± 0.027
2.004ArgTyr: 2.004 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
5.257SerAla: 5.257 ± 0.087
0.592SerCys: 0.592 ± 0.023
3.418SerAsp: 3.418 ± 0.062
3.424SerGlu: 3.424 ± 0.062
2.476SerPhe: 2.476 ± 0.053
4.968SerGly: 4.968 ± 0.082
1.518SerHis: 1.518 ± 0.044
4.282SerIle: 4.282 ± 0.069
2.714SerLys: 2.714 ± 0.059
6.362SerLeu: 6.362 ± 0.094
1.47SerMet: 1.47 ± 0.04
2.819SerAsn: 2.819 ± 0.066
2.61SerPro: 2.61 ± 0.057
2.448SerGln: 2.448 ± 0.053
3.085SerArg: 3.085 ± 0.058
3.825SerSer: 3.825 ± 0.067
3.246SerThr: 3.246 ± 0.081
4.073SerVal: 4.073 ± 0.08
0.778SerTrp: 0.778 ± 0.025
1.71SerTyr: 1.71 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
4.814ThrAla: 4.814 ± 0.099
0.531ThrCys: 0.531 ± 0.023
3.133ThrAsp: 3.133 ± 0.081
2.984ThrGlu: 2.984 ± 0.057
2.118ThrPhe: 2.118 ± 0.052
4.292ThrGly: 4.292 ± 0.093
1.29ThrHis: 1.29 ± 0.037
3.831ThrIle: 3.831 ± 0.085
2.173ThrLys: 2.173 ± 0.053
6.351ThrLeu: 6.351 ± 0.151
1.182ThrMet: 1.182 ± 0.032
2.138ThrAsn: 2.138 ± 0.055
2.653ThrPro: 2.653 ± 0.052
2.215ThrGln: 2.215 ± 0.057
2.619ThrArg: 2.619 ± 0.053
3.119ThrSer: 3.119 ± 0.069
2.978ThrThr: 2.978 ± 0.059
4.048ThrVal: 4.048 ± 0.133
0.578ThrTrp: 0.578 ± 0.021
1.515ThrTyr: 1.515 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
5.854ValAla: 5.854 ± 0.093
0.626ValCys: 0.626 ± 0.025
3.662ValAsp: 3.662 ± 0.066
3.804ValGlu: 3.804 ± 0.057
2.692ValPhe: 2.692 ± 0.055
4.043ValGly: 4.043 ± 0.068
1.381ValHis: 1.381 ± 0.036
4.685ValIle: 4.685 ± 0.065
3.2ValLys: 3.2 ± 0.069
6.63ValLeu: 6.63 ± 0.099
1.742ValMet: 1.742 ± 0.044
2.939ValAsn: 2.939 ± 0.077
2.775ValPro: 2.775 ± 0.052
2.197ValGln: 2.197 ± 0.047
3.27ValArg: 3.27 ± 0.062
4.257ValSer: 4.257 ± 0.064
4.051ValThr: 4.051 ± 0.113
4.52ValVal: 4.52 ± 0.078
0.721ValTrp: 0.721 ± 0.025
1.734ValTyr: 1.734 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
0.804TrpAla: 0.804 ± 0.029
0.149TrpCys: 0.149 ± 0.011
0.6TrpAsp: 0.6 ± 0.029
0.654TrpGlu: 0.654 ± 0.026
0.594TrpPhe: 0.594 ± 0.026
0.774TrpGly: 0.774 ± 0.025
0.382TrpHis: 0.382 ± 0.02
0.825TrpIle: 0.825 ± 0.027
0.569TrpLys: 0.569 ± 0.026
1.727TrpLeu: 1.727 ± 0.048
0.318TrpMet: 0.318 ± 0.018
0.515TrpAsn: 0.515 ± 0.026
0.496TrpPro: 0.496 ± 0.025
0.82TrpGln: 0.82 ± 0.031
0.921TrpArg: 0.921 ± 0.031
0.67TrpSer: 0.67 ± 0.026
0.556TrpThr: 0.556 ± 0.025
0.804TrpVal: 0.804 ± 0.029
0.213TrpTrp: 0.213 ± 0.017
0.336TrpTyr: 0.336 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.492TyrAla: 2.492 ± 0.056
0.363TyrCys: 0.363 ± 0.018
1.592TyrAsp: 1.592 ± 0.044
1.436TyrGlu: 1.436 ± 0.04
1.352TyrPhe: 1.352 ± 0.033
2.045TyrGly: 2.045 ± 0.041
0.843TyrHis: 0.843 ± 0.031
1.629TyrIle: 1.629 ± 0.041
1.145TyrLys: 1.145 ± 0.034
3.339TyrLeu: 3.339 ± 0.067
0.587TyrMet: 0.587 ± 0.027
1.117TyrAsn: 1.117 ± 0.039
1.447TyrPro: 1.447 ± 0.039
1.542TyrGln: 1.542 ± 0.041
2.016TyrArg: 2.016 ± 0.049
1.79TyrSer: 1.79 ± 0.044
1.519TyrThr: 1.519 ± 0.046
1.703TyrVal: 1.703 ± 0.039
0.48TyrTrp: 0.48 ± 0.023
0.969TyrTyr: 0.969 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.001XaaArg: 0.001 ± 0.001
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3249 proteins (1053642 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski