Arthrobacter sp. AQ5-06
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3663 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A329YWA5|A0A329YWA5_9MICC Dioxygenase OS=Arthrobacter sp. AQ5-06 OX=1914304 GN=DQ354_05900 PE=4 SV=1
MM1 pKa = 7.26 SRR3 pKa = 11.84 ALIIVDD9 pKa = 3.67 VQNDD13 pKa = 3.63 FCEE16 pKa = 4.88 GGSLAVAGGADD27 pKa = 3.35 VAGAISEE34 pKa = 4.4 YY35 pKa = 11.07 VDD37 pKa = 3.12 AHH39 pKa = 6.32 HH40 pKa = 7.08 NEE42 pKa = 3.83 FDD44 pKa = 4.95 HH45 pKa = 7.69 IVATQDD51 pKa = 2.79 WHH53 pKa = 6.82 IDD55 pKa = 3.55 PGAHH59 pKa = 7.19 FSDD62 pKa = 4.03 TPDD65 pKa = 5.22 LKK67 pKa = 11.15 DD68 pKa = 2.99 SWPRR72 pKa = 11.84 HH73 pKa = 5.09 CVAGTRR79 pKa = 11.84 GAEE82 pKa = 3.94 LHH84 pKa = 7.04 PDD86 pKa = 3.53 LDD88 pKa = 3.77 TEE90 pKa = 4.48 YY91 pKa = 10.49 IQAYY95 pKa = 5.92 FQKK98 pKa = 10.57 GQFAAAYY105 pKa = 9.64 SGFEE109 pKa = 3.96 GLLAPEE115 pKa = 4.76 DD116 pKa = 3.76 AVPTGEE122 pKa = 4.18 RR123 pKa = 11.84 QPGAMPADD131 pKa = 3.82 ADD133 pKa = 3.94 AYY135 pKa = 10.75 APADD139 pKa = 3.68 NAIGLDD145 pKa = 3.67 DD146 pKa = 4.2 WLQSHH151 pKa = 6.64 DD152 pKa = 3.82 VEE154 pKa = 4.63 DD155 pKa = 4.06 VVVVGIATDD164 pKa = 3.76 YY165 pKa = 11.34 CVMATSLDD173 pKa = 3.78 AVQAGYY179 pKa = 10.47 SVTVIRR185 pKa = 11.84 SLTAGIAEE193 pKa = 4.39 DD194 pKa = 4.94 LEE196 pKa = 4.53 DD197 pKa = 4.4 AVAEE201 pKa = 4.13 MEE203 pKa = 4.3 LGGVEE208 pKa = 4.36 VAA210 pKa = 5.23
Molecular weight: 22.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.986
Patrickios 0.896
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A329YNQ4|A0A329YNQ4_9MICC Threonine/serine exporter family protein OS=Arthrobacter sp. AQ5-06 OX=1914304 GN=DQ354_12960 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.31 VRR4 pKa = 11.84 NSLRR8 pKa = 11.84 ALKK11 pKa = 9.72 KK12 pKa = 10.42 VPGAQIVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 TFVINKK30 pKa = 9.03 KK31 pKa = 8.56 NPRR34 pKa = 11.84 MKK36 pKa = 10.47 ARR38 pKa = 11.84 QGG40 pKa = 3.24
Molecular weight: 4.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.618
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.34
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3663
0
3663
1169135
24
3558
319.2
34.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.029 ± 0.056
0.628 ± 0.011
5.642 ± 0.036
5.544 ± 0.037
3.225 ± 0.027
9.081 ± 0.035
2.099 ± 0.02
4.408 ± 0.031
2.821 ± 0.03
10.24 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.042 ± 0.017
2.523 ± 0.026
5.425 ± 0.033
3.174 ± 0.022
6.427 ± 0.038
5.948 ± 0.028
5.91 ± 0.031
8.357 ± 0.036
1.408 ± 0.02
2.07 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here