Pontivivens insulae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pontivivens

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3498 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R8AF50|A0A2R8AF50_9RHOB Beta-ketodecanoyl-[acyl-carrier-protein] synthase OS=Pontivivens insulae OX=1639689 GN=POI8812_03219 PE=4 SV=1
MM1 pKa = 7.36SFSKK5 pKa = 8.03TTAIAALMTAAAALPAAAQDD25 pKa = 3.99LCGGAAANGTWIGGSDD41 pKa = 3.85EE42 pKa = 4.38TSDD45 pKa = 3.25IASGFGPFDD54 pKa = 3.24MTAQVPAGGEE64 pKa = 3.8HH65 pKa = 4.98VAIFNVSTASDD76 pKa = 3.74VRR78 pKa = 11.84VEE80 pKa = 4.11AAPVADD86 pKa = 4.21GDD88 pKa = 4.48TVIDD92 pKa = 4.4LLDD95 pKa = 3.83ASGVVILSDD104 pKa = 3.8DD105 pKa = 4.02DD106 pKa = 4.55GGGFLASRR114 pKa = 11.84GEE116 pKa = 3.81ISLDD120 pKa = 3.11PGTYY124 pKa = 10.17CLATRR129 pKa = 11.84SYY131 pKa = 11.88DD132 pKa = 4.01DD133 pKa = 3.9AALTADD139 pKa = 3.54VRR141 pKa = 11.84VGLTVHH147 pKa = 7.18DD148 pKa = 5.72PITSGGFNDD157 pKa = 3.96YY158 pKa = 11.2DD159 pKa = 3.83AVCLPEE165 pKa = 4.1TEE167 pKa = 4.31AVSFGGTNLNEE178 pKa = 4.02GLEE181 pKa = 4.32AGVAATASIGDD192 pKa = 3.63TPFYY196 pKa = 10.46RR197 pKa = 11.84FNLSEE202 pKa = 4.09PTSISFTAEE211 pKa = 3.73NQSADD216 pKa = 3.87PILRR220 pKa = 11.84IFDD223 pKa = 3.34NTGYY227 pKa = 9.66MLAEE231 pKa = 4.22NDD233 pKa = 5.1DD234 pKa = 4.01YY235 pKa = 12.03DD236 pKa = 4.17GLNSRR241 pKa = 11.84LDD243 pKa = 3.57FVQPLEE249 pKa = 4.35AGTYY253 pKa = 9.91CIGLIALSDD262 pKa = 3.66PGAPVTVTANAFSEE276 pKa = 3.98QDD278 pKa = 3.71YY279 pKa = 11.71LEE281 pKa = 4.2TLYY284 pKa = 11.49NNAEE288 pKa = 4.18TSPPLDD294 pKa = 3.0GSYY297 pKa = 10.76PVTDD301 pKa = 4.71LGLISTRR308 pKa = 11.84ARR310 pKa = 11.84ADD312 pKa = 3.14VRR314 pKa = 11.84VDD316 pKa = 5.28DD317 pKa = 5.05DD318 pKa = 5.65LIWHH322 pKa = 6.17QFQVDD327 pKa = 3.85QPGLILIEE335 pKa = 4.79GIGTGGVDD343 pKa = 4.58AMLTLFDD350 pKa = 4.75DD351 pKa = 5.05FGRR354 pKa = 11.84MITQNDD360 pKa = 4.35DD361 pKa = 3.26YY362 pKa = 11.23GQSLDD367 pKa = 3.52AQIAARR373 pKa = 11.84VQPGTYY379 pKa = 9.17VLAVGRR385 pKa = 11.84PADD388 pKa = 3.67YY389 pKa = 10.04FYY391 pKa = 11.38GEE393 pKa = 4.14PGITRR398 pKa = 11.84VGIEE402 pKa = 4.01MFVSARR408 pKa = 3.45

Molecular weight: 42.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R8AFQ0|A0A2R8AFQ0_9RHOB Uncharacterized protein OS=Pontivivens insulae OX=1639689 GN=POI8812_03424 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84KK29 pKa = 9.03ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3498

0

3498

1084088

29

11983

309.9

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.497 ± 0.06

0.895 ± 0.016

6.235 ± 0.051

6.037 ± 0.044

3.718 ± 0.027

8.665 ± 0.051

2.04 ± 0.026

5.273 ± 0.027

2.734 ± 0.037

10.021 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.792 ± 0.029

2.598 ± 0.027

4.958 ± 0.028

3.265 ± 0.023

6.686 ± 0.055

5.173 ± 0.032

5.541 ± 0.05

7.306 ± 0.036

1.413 ± 0.02

2.154 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski