Ceratobasidium theobromae
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9256 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N5QEB7|A0A5N5QEB7_9AGAM Mitogen-activated protein kinase OS=Ceratobasidium theobromae OX=1582974 GN=CTheo_6759 PE=3 SV=1
MM1 pKa = 7.58 GISYY5 pKa = 10.41 YY6 pKa = 10.78 DD7 pKa = 3.7 EE8 pKa = 5.76 IEE10 pKa = 4.5 IEE12 pKa = 4.19 DD13 pKa = 4.18 MVWDD17 pKa = 3.77 EE18 pKa = 4.4 AKK20 pKa = 10.17 RR21 pKa = 11.84 VYY23 pKa = 10.17 HH24 pKa = 5.7 YY25 pKa = 8.21 PCPCGDD31 pKa = 3.19 RR32 pKa = 11.84 FEE34 pKa = 4.61 ISRR37 pKa = 11.84 EE38 pKa = 3.64 QLANYY43 pKa = 9.4 EE44 pKa = 4.92 DD45 pKa = 4.61 IATCPSCSLIIRR57 pKa = 11.84 VIYY60 pKa = 10.39 DD61 pKa = 3.4 PLDD64 pKa = 4.11 FEE66 pKa = 6.0 DD67 pKa = 5.03 EE68 pKa = 4.59 EE69 pKa = 5.4 PDD71 pKa = 4.08 EE72 pKa = 5.69 DD73 pKa = 5.0 DD74 pKa = 4.56 PDD76 pKa = 5.77 PIEE79 pKa = 4.48 EE80 pKa = 4.37 EE81 pKa = 4.23 EE82 pKa = 4.94 SSGSDD87 pKa = 4.04 DD88 pKa = 4.36 GGKK91 pKa = 9.71 EE92 pKa = 3.62 ADD94 pKa = 3.43 KK95 pKa = 11.03 VQNAMEE101 pKa = 4.11 NLALNEE107 pKa = 3.96 EE108 pKa = 4.48 PLAVTLVEE116 pKa = 4.43 TRR118 pKa = 11.84 AA119 pKa = 3.38
Molecular weight: 13.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.49
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.567
Rodwell 3.516
Grimsley 3.401
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.948
Thurlkill 3.541
EMBOSS 3.579
Sillero 3.808
Patrickios 0.985
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.727
Protein with the highest isoelectric point:
>tr|A0A5N5QQ55|A0A5N5QQ55_9AGAM G protein coupled receptor OS=Ceratobasidium theobromae OX=1582974 GN=CTheo_3289 PE=4 SV=1
MM1 pKa = 7.36 SLSPPQPLRR10 pKa = 11.84 ARR12 pKa = 11.84 ASSIVQPLTIDD23 pKa = 3.26 SLRR26 pKa = 11.84 RR27 pKa = 11.84 TTGLATPPASPFSSVFTRR45 pKa = 11.84 PPRR48 pKa = 11.84 QHH50 pKa = 6.7 LPSPALSRR58 pKa = 11.84 TPSPPPRR65 pKa = 11.84 EE66 pKa = 3.51 THH68 pKa = 6.73 APVLRR73 pKa = 11.84 LAAGFALSAVPILMALSTLPVALRR97 pKa = 11.84 MPRR100 pKa = 11.84 TLADD104 pKa = 3.04 IALLGRR110 pKa = 11.84 DD111 pKa = 3.77 LKK113 pKa = 11.13 AYY115 pKa = 9.14 AASAPGMAHH124 pKa = 6.15 VMAVMCCLAVWEE136 pKa = 4.92 HH137 pKa = 6.2 AWSIPGSVVLNVLAGALVSPLWATLLMTLLTSLGSLAASLLAAPLEE183 pKa = 4.75 PIVQRR188 pKa = 11.84 IAPKK192 pKa = 10.49 ALDD195 pKa = 3.68 VVASALQGDD204 pKa = 3.8 PRR206 pKa = 11.84 AAKK209 pKa = 7.9 RR210 pKa = 11.84 TPTWVRR216 pKa = 11.84 IVVMRR221 pKa = 11.84 LVGVVPWSAINIACGVCRR239 pKa = 11.84 VPFVDD244 pKa = 3.98 CAIGAFIGALPWTAVTCQTLALGSSSDD271 pKa = 3.63 PKK273 pKa = 10.41 TLSSLLSSPAIILKK287 pKa = 10.36 LVLLSLLSLGPVLCRR302 pKa = 11.84 DD303 pKa = 3.71 KK304 pKa = 11.19 LTDD307 pKa = 3.63 ALAATDD313 pKa = 4.23 EE314 pKa = 4.39 KK315 pKa = 11.46 APLAPAPMWKK325 pKa = 8.53 VWHH328 pKa = 6.24 WRR330 pKa = 11.84 RR331 pKa = 11.84 SSHH334 pKa = 5.99 GLPRR338 pKa = 11.84 PKK340 pKa = 10.47 SPEE343 pKa = 3.61 RR344 pKa = 11.84 TMEE347 pKa = 3.9 EE348 pKa = 4.04 VLDD351 pKa = 4.18 EE352 pKa = 4.17 NLIFAMSPHH361 pKa = 7.02 ANHH364 pKa = 5.57 VMYY367 pKa = 9.99 PVLLFCSGGIQSSSLVYY384 pKa = 10.57 NYY386 pKa = 10.2 LIQFGLGRR394 pKa = 11.84 LEE396 pKa = 4.64 PGPLGIPRR404 pKa = 11.84 STHH407 pKa = 6.24 PNTGRR412 pKa = 11.84 HH413 pKa = 5.09 PAYY416 pKa = 10.25 ALSQFSFQHH425 pKa = 5.85 PPVRR429 pKa = 11.84 PHH431 pKa = 6.82 LARR434 pKa = 11.84 RR435 pKa = 11.84 SHH437 pKa = 6.91 FDD439 pKa = 3.06 VTRR442 pKa = 11.84 TTWLAYY448 pKa = 9.54 KK449 pKa = 10.01 FASKK453 pKa = 10.53 GPHH456 pKa = 6.33 IFLCPASAPRR466 pKa = 11.84 VSRR469 pKa = 11.84 PSLRR473 pKa = 3.52
Molecular weight: 50.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 9.545
IPC_protein 10.365
Toseland 10.584
ProMoST 10.438
Dawson 10.687
Bjellqvist 10.438
Wikipedia 10.906
Rodwell 10.833
Grimsley 10.745
Solomon 10.818
Lehninger 10.774
Nozaki 10.613
DTASelect 10.409
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.643
Patrickios 10.54
IPC_peptide 10.818
IPC2_peptide 9.75
IPC2.peptide.svr19 8.587
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9256
0
9256
5421178
66
6167
585.7
64.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.943 ± 0.021
1.234 ± 0.008
5.418 ± 0.016
5.688 ± 0.025
3.501 ± 0.014
6.899 ± 0.025
2.513 ± 0.01
4.944 ± 0.016
4.322 ± 0.02
8.998 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.027 ± 0.009
3.502 ± 0.013
6.807 ± 0.031
3.735 ± 0.013
6.29 ± 0.021
8.668 ± 0.027
6.181 ± 0.016
6.337 ± 0.015
1.466 ± 0.009
2.53 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here