Elizabethkingia anophelis NUHP1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Elizabethkingia; Elizabethkingia anophelis

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077ECN5|A0A077ECN5_9FLAO Arsenical resistance operon repressor OS=Elizabethkingia anophelis NUHP1 OX=1338011 GN=BD94_1536 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 6.21DD3 pKa = 3.09EE4 pKa = 5.02AFYY7 pKa = 11.56AMRR10 pKa = 11.84NQKK13 pKa = 10.4DD14 pKa = 3.38GAKK17 pKa = 10.43GIFINDD23 pKa = 3.51YY24 pKa = 7.74STCAANSIIDD34 pKa = 3.61QMSVYY39 pKa = 10.79EE40 pKa = 5.0DD41 pKa = 4.48GNNDD45 pKa = 2.86WTIEE49 pKa = 3.98NN50 pKa = 4.02

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077E982|A0A077E982_9FLAO Uncharacterized protein OS=Elizabethkingia anophelis NUHP1 OX=1338011 GN=BD94_0302 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.55RR3 pKa = 11.84TFQPSEE9 pKa = 3.58RR10 pKa = 11.84KK11 pKa = 9.37KK12 pKa = 10.2RR13 pKa = 11.84NKK15 pKa = 9.71HH16 pKa = 4.09GFRR19 pKa = 11.84EE20 pKa = 4.33RR21 pKa = 11.84MSTPNGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.76RR41 pKa = 11.84LTVSASRR48 pKa = 11.84AKK50 pKa = 10.41RR51 pKa = 3.34

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4061

0

4061

1281627

37

1947

315.6

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.306 ± 0.039

0.698 ± 0.011

5.364 ± 0.028

6.499 ± 0.044

5.211 ± 0.03

6.37 ± 0.039

1.666 ± 0.018

7.917 ± 0.036

8.326 ± 0.045

9.073 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.019

6.294 ± 0.035

3.455 ± 0.02

3.691 ± 0.024

3.489 ± 0.025

6.462 ± 0.027

5.453 ± 0.027

5.87 ± 0.032

1.093 ± 0.013

4.431 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski