Lamium leaf distortion virus
Average proteome isoelectric point is 7.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B2CXY5|B2CXY5_9VIRU Aspartic protease OS=Lamium leaf distortion virus OX=515320 PE=3 SV=1
MM1 pKa = 7.49 SFYY4 pKa = 11.2 EE5 pKa = 5.51 LIDD8 pKa = 3.46 KK9 pKa = 8.65 QFWEE13 pKa = 4.23 TLEE16 pKa = 4.31 TQSEE20 pKa = 4.11 LDD22 pKa = 3.36 EE23 pKa = 4.51 RR24 pKa = 11.84 LNTLSRR30 pKa = 11.84 LVNQGEE36 pKa = 4.11 QATNEE41 pKa = 3.95 EE42 pKa = 4.27 LQGNEE47 pKa = 3.91 TLRR50 pKa = 11.84 SFLNLDD56 pKa = 4.04 NICEE60 pKa = 4.09 RR61 pKa = 11.84 FEE63 pKa = 4.03 TLNVIEE69 pKa = 4.93 EE70 pKa = 4.22 SSEE73 pKa = 4.28 DD74 pKa = 3.88 SSIDD78 pKa = 3.3 NNDD81 pKa = 3.36 PVDD84 pKa = 3.88 YY85 pKa = 10.39 SDD87 pKa = 4.02 SSSEE91 pKa = 4.17 EE92 pKa = 3.94 EE93 pKa = 3.95 EE94 pKa = 4.03 QNTNKK99 pKa = 10.09 RR100 pKa = 11.84 FFMEE104 pKa = 4.46 KK105 pKa = 10.87 GEE107 pKa = 4.2 GSQNKK112 pKa = 9.0 KK113 pKa = 10.82 DD114 pKa = 3.72 KK115 pKa = 10.44 DD116 pKa = 3.75 PKK118 pKa = 10.01 VEE120 pKa = 4.03 YY121 pKa = 10.17 PNVEE125 pKa = 4.0 NLQSEE130 pKa = 4.75 DD131 pKa = 3.25 NRR133 pKa = 11.84 RR134 pKa = 11.84 TPFNNNKK141 pKa = 9.69 RR142 pKa = 11.84 KK143 pKa = 10.12 SPEE146 pKa = 3.52 QARR149 pKa = 11.84 YY150 pKa = 7.61 PVKK153 pKa = 10.48 PKK155 pKa = 10.65 FEE157 pKa = 4.41 VVSRR161 pKa = 11.84 TLGFLDD167 pKa = 4.79 IDD169 pKa = 4.34 CQDD172 pKa = 3.3 HH173 pKa = 5.91 EE174 pKa = 4.56 VRR176 pKa = 11.84 KK177 pKa = 10.55 KK178 pKa = 10.85 NIDD181 pKa = 2.9 AWASEE186 pKa = 4.15 LSLIVQTNPDD196 pKa = 3.57 AYY198 pKa = 10.62 DD199 pKa = 3.51 NADD202 pKa = 3.46 AVILLAEE209 pKa = 4.68 HH210 pKa = 6.86 KK211 pKa = 10.79 ALGNAKK217 pKa = 9.57 EE218 pKa = 4.47 LIKK221 pKa = 10.16 GTYY224 pKa = 7.33 WNKK227 pKa = 10.65 DD228 pKa = 3.47 NPPMYY233 pKa = 9.66 MLNQIFDD240 pKa = 3.79 SLYY243 pKa = 11.34 SMFLGLDD250 pKa = 3.35 YY251 pKa = 11.16 TDD253 pKa = 5.61 AISKK257 pKa = 10.29 LLEE260 pKa = 3.94 TNKK263 pKa = 9.97 QKK265 pKa = 11.01 EE266 pKa = 4.02 NARR269 pKa = 11.84 IRR271 pKa = 11.84 LANLKK276 pKa = 9.81 LCNICSIDD284 pKa = 3.87 EE285 pKa = 4.29 FNCAYY290 pKa = 10.18 EE291 pKa = 4.22 EE292 pKa = 4.26 EE293 pKa = 4.42 FFKK296 pKa = 11.18 LNQNEE301 pKa = 4.15 YY302 pKa = 9.46 PKK304 pKa = 10.9 YY305 pKa = 10.27 ISDD308 pKa = 4.33 YY309 pKa = 9.85 LLKK312 pKa = 10.53 IPVVGLAAKK321 pKa = 10.13 EE322 pKa = 4.28 KK323 pKa = 10.13 FDD325 pKa = 3.76 KK326 pKa = 8.95 TTGTLAYY333 pKa = 10.4 SIGHH337 pKa = 6.0 AEE339 pKa = 3.82 KK340 pKa = 10.54 LVRR343 pKa = 11.84 EE344 pKa = 4.85 EE345 pKa = 3.53 ITKK348 pKa = 9.89 ICEE351 pKa = 3.98 LSQQQKK357 pKa = 10.19 KK358 pKa = 9.82 LKK360 pKa = 10.15 KK361 pKa = 9.84 ISKK364 pKa = 9.14 KK365 pKa = 10.05 CCNKK369 pKa = 9.77 IANKK373 pKa = 8.93 STDD376 pKa = 2.95 IGCYY380 pKa = 5.09 TTKK383 pKa = 10.43 SYY385 pKa = 11.09 KK386 pKa = 9.89 KK387 pKa = 9.91 KK388 pKa = 10.17 KK389 pKa = 9.02 KK390 pKa = 10.17 KK391 pKa = 10.08 KK392 pKa = 7.41 FKK394 pKa = 10.49 KK395 pKa = 10.03 YY396 pKa = 10.13 VFSKK400 pKa = 10.49 RR401 pKa = 11.84 KK402 pKa = 8.95 RR403 pKa = 11.84 KK404 pKa = 9.58 KK405 pKa = 9.9 FRR407 pKa = 11.84 PGKK410 pKa = 9.67 YY411 pKa = 8.43 FQKK414 pKa = 10.77 KK415 pKa = 9.12 KK416 pKa = 10.61 FPTSGEE422 pKa = 4.19 GKK424 pKa = 10.14 KK425 pKa = 9.89 PCPKK429 pKa = 10.55 GKK431 pKa = 8.6 TNCRR435 pKa = 11.84 CWICNVEE442 pKa = 3.6 GHH444 pKa = 6.06 YY445 pKa = 11.17 ANKK448 pKa = 10.24 CPNKK452 pKa = 9.64 QKK454 pKa = 10.78 YY455 pKa = 8.2 GEE457 pKa = 4.21 KK458 pKa = 10.6 VKK460 pKa = 10.47 ILRR463 pKa = 11.84 KK464 pKa = 9.74 ADD466 pKa = 3.76 LQDD469 pKa = 3.97 LEE471 pKa = 4.8 PIEE474 pKa = 5.77 DD475 pKa = 3.55 IFEE478 pKa = 4.37 EE479 pKa = 4.31 SLDD482 pKa = 3.72 VYY484 pKa = 10.18 IAEE487 pKa = 4.92 IIEE490 pKa = 4.08 FDD492 pKa = 3.94 PDD494 pKa = 3.24 LSYY497 pKa = 11.34 QEE499 pKa = 5.15 SSDD502 pKa = 3.74 EE503 pKa = 4.15 SSEE506 pKa = 4.15 EE507 pKa = 3.82 SDD509 pKa = 3.38
Molecular weight: 59.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.576
IPC2_protein 5.817
IPC_protein 5.779
Toseland 5.779
ProMoST 5.855
Dawson 5.868
Bjellqvist 5.957
Wikipedia 5.779
Rodwell 5.817
Grimsley 5.753
Solomon 5.868
Lehninger 5.842
Nozaki 6.046
DTASelect 6.186
Thurlkill 6.033
EMBOSS 5.982
Sillero 6.135
Patrickios 4.291
IPC_peptide 5.881
IPC2_peptide 6.059
IPC2.peptide.svr19 6.351
Protein with the highest isoelectric point:
>tr|B2CXY3|B2CXY3_9VIRU Protein 3 OS=Lamium leaf distortion virus OX=515320 PE=3 SV=1
MM1 pKa = 7.6 SITSKK6 pKa = 10.2 SHH8 pKa = 5.85 IYY10 pKa = 10.44 KK11 pKa = 10.38 KK12 pKa = 10.03 GTTIIPLKK20 pKa = 10.49 NLAINTDD27 pKa = 2.98 GKK29 pKa = 11.11 KK30 pKa = 10.32 YY31 pKa = 10.72 VFNSLKK37 pKa = 10.62 PNIQSVVNHH46 pKa = 6.23 CNNLNEE52 pKa = 4.13 VCGRR56 pKa = 11.84 ILLGIWKK63 pKa = 8.92 LCSYY67 pKa = 10.58 FGLSKK72 pKa = 10.9 DD73 pKa = 3.27 PSEE76 pKa = 4.14 PHH78 pKa = 6.15 SKK80 pKa = 10.46 NPSVYY85 pKa = 10.7 DD86 pKa = 3.41 HH87 pKa = 7.27 AKK89 pKa = 10.03 TIFKK93 pKa = 10.62 SGGVDD98 pKa = 2.85 HH99 pKa = 6.9 SVILRR104 pKa = 11.84 EE105 pKa = 3.91 IKK107 pKa = 10.64 SLIEE111 pKa = 3.81 TQQTKK116 pKa = 10.53 NKK118 pKa = 9.77 NLEE121 pKa = 4.02 NKK123 pKa = 9.49 IDD125 pKa = 3.86 NLEE128 pKa = 4.03 KK129 pKa = 10.59 SIKK132 pKa = 10.06 DD133 pKa = 3.41 LSHH136 pKa = 7.11 KK137 pKa = 10.31 IEE139 pKa = 4.67 PEE141 pKa = 3.81 PLTHH145 pKa = 6.97 EE146 pKa = 4.66 KK147 pKa = 10.45 IKK149 pKa = 11.05 DD150 pKa = 3.39 FSNALKK156 pKa = 10.81 AIDD159 pKa = 4.99 DD160 pKa = 3.84 KK161 pKa = 11.42 LKK163 pKa = 11.15 NVIGEE168 pKa = 4.08
Molecular weight: 18.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.764
IPC2_protein 8.492
IPC_protein 8.375
Toseland 9.355
ProMoST 8.96
Dawson 9.531
Bjellqvist 9.238
Wikipedia 9.633
Rodwell 10.101
Grimsley 9.545
Solomon 9.604
Lehninger 9.589
Nozaki 9.502
DTASelect 9.165
Thurlkill 9.428
EMBOSS 9.75
Sillero 9.516
Patrickios 7.629
IPC_peptide 9.604
IPC2_peptide 7.761
IPC2.peptide.svr19 7.858
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2335
122
696
389.2
44.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.411 ± 0.242
1.97 ± 0.261
5.396 ± 0.432
7.923 ± 0.726
4.368 ± 0.306
3.94 ± 0.22
2.184 ± 0.401
7.666 ± 0.811
11.221 ± 0.673
8.822 ± 0.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.627 ± 0.274
6.51 ± 0.859
4.411 ± 0.66
4.069 ± 0.3
3.683 ± 0.402
8.223 ± 0.834
4.668 ± 0.172
4.668 ± 0.35
0.857 ± 0.172
3.383 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here