Propioniciclava tarda
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2684 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q9KJE0|A0A4Q9KJE0_9ACTN VOC family protein OS=Propioniciclava tarda OX=433330 GN=ET996_10155 PE=4 SV=1
MM1 pKa = 7.63 PVTMSEE7 pKa = 4.01 EE8 pKa = 3.67 RR9 pKa = 11.84 FAALVEE15 pKa = 4.08 QALDD19 pKa = 4.73 DD20 pKa = 4.37 IPDD23 pKa = 3.87 EE24 pKa = 4.32 LSSLIEE30 pKa = 3.88 NCLIVVDD37 pKa = 5.22 DD38 pKa = 5.36 DD39 pKa = 5.49 APADD43 pKa = 4.2 DD44 pKa = 5.58 PDD46 pKa = 5.12 LLGLYY51 pKa = 10.3 DD52 pKa = 5.49 GIPLTEE58 pKa = 4.22 RR59 pKa = 11.84 GMDD62 pKa = 3.45 YY63 pKa = 10.87 SGYY66 pKa = 10.68 LPDD69 pKa = 4.61 TIHH72 pKa = 6.87 IFRR75 pKa = 11.84 RR76 pKa = 11.84 NILAACDD83 pKa = 3.64 TEE85 pKa = 4.19 TDD87 pKa = 3.76 VVEE90 pKa = 4.12 EE91 pKa = 4.26 VYY93 pKa = 9.33 ITVVHH98 pKa = 6.2 EE99 pKa = 3.98 VAHH102 pKa = 6.16 FFGIDD107 pKa = 3.5 DD108 pKa = 3.89 EE109 pKa = 5.32 RR110 pKa = 11.84 LDD112 pKa = 3.83 EE113 pKa = 5.21 LGYY116 pKa = 11.23 AA117 pKa = 4.27
Molecular weight: 13.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.656
IPC_protein 3.643
Toseland 3.439
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.592
Rodwell 3.478
Grimsley 3.35
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.986
Thurlkill 3.49
EMBOSS 3.592
Sillero 3.77
Patrickios 0.947
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A4Q9KLZ3|A0A4Q9KLZ3_9ACTN Argininosuccinate lyase OS=Propioniciclava tarda OX=433330 GN=argH PE=3 SV=1
MM1 pKa = 7.46 PRR3 pKa = 11.84 HH4 pKa = 5.46 RR5 pKa = 11.84 TRR7 pKa = 11.84 SRR9 pKa = 11.84 TPAQRR14 pKa = 11.84 TPRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 PHH22 pKa = 5.89 PRR24 pKa = 11.84 PPRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 GRR31 pKa = 11.84 PRR33 pKa = 11.84 PRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 ARR39 pKa = 11.84 PPAQRR44 pKa = 11.84 QPRR47 pKa = 11.84 HH48 pKa = 6.31 RR49 pKa = 11.84 GRR51 pKa = 11.84 GGAPWRR57 pKa = 11.84 LRR59 pKa = 11.84 DD60 pKa = 3.69 RR61 pKa = 11.84 RR62 pKa = 11.84 GGRR65 pKa = 11.84 PP66 pKa = 3.08
Molecular weight: 8.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.618
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.325
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.278
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2684
0
2684
874148
24
2381
325.7
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.589 ± 0.066
0.794 ± 0.013
6.16 ± 0.036
5.041 ± 0.041
2.986 ± 0.026
8.909 ± 0.043
2.002 ± 0.021
4.113 ± 0.031
2.497 ± 0.038
10.312 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.943 ± 0.024
2.039 ± 0.024
5.59 ± 0.038
2.811 ± 0.025
7.158 ± 0.057
5.609 ± 0.033
5.99 ± 0.045
8.931 ± 0.048
1.52 ± 0.019
2.005 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here