Streptococcus satellite phage Javan321

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJD9|A0A4D5ZJD9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan321 OX=2558634 GN=JavanS321_0020 PE=4 SV=1
MM1 pKa = 6.76EE2 pKa = 4.99HH3 pKa = 6.7ARR5 pKa = 11.84VIRR8 pKa = 11.84IIDD11 pKa = 4.3DD12 pKa = 3.37FTIMIDD18 pKa = 3.27RR19 pKa = 11.84GYY21 pKa = 9.25DD22 pKa = 2.98TSDD25 pKa = 3.04IEE27 pKa = 4.21EE28 pKa = 4.35GTRR31 pKa = 11.84ITVFEE36 pKa = 5.05PGPEE40 pKa = 3.99IKK42 pKa = 10.66DD43 pKa = 3.41LDD45 pKa = 4.17GNSLGKK51 pKa = 10.49YY52 pKa = 10.26DD53 pKa = 3.66FTKK56 pKa = 10.94ANLIITEE63 pKa = 4.08VFQRR67 pKa = 11.84FSIAQNIEE75 pKa = 3.66EE76 pKa = 4.58ASPFSVTNFLSGGQAKK92 pKa = 9.89LVINVNEE99 pKa = 4.41DD100 pKa = 3.45EE101 pKa = 4.85IEE103 pKa = 4.1PLEE106 pKa = 4.45PKK108 pKa = 10.3DD109 pKa = 3.53IQVKK113 pKa = 10.51KK114 pKa = 10.52GDD116 pKa = 3.97LVRR119 pKa = 11.84IDD121 pKa = 4.52DD122 pKa = 3.92FF123 pKa = 5.78

Molecular weight: 13.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZJ98|A0A4D5ZJ98_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan321 OX=2558634 GN=JavanS321_0022 PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 5.23NLIEE6 pKa = 4.51SDD8 pKa = 3.63LVIFKK13 pKa = 9.43MRR15 pKa = 11.84YY16 pKa = 9.19LEE18 pKa = 5.9AGATWEE24 pKa = 4.69DD25 pKa = 3.61VAEE28 pKa = 4.52KK29 pKa = 10.76LNKK32 pKa = 5.44TTRR35 pKa = 11.84YY36 pKa = 8.12INSRR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 9.58VIAKK46 pKa = 9.92RR47 pKa = 11.84FVEE50 pKa = 4.28LKK52 pKa = 10.67GYY54 pKa = 10.26

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

4210

37

415

155.9

18.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.273 ± 0.607

0.641 ± 0.153

6.508 ± 0.476

8.622 ± 0.503

4.347 ± 0.365

4.703 ± 0.315

1.568 ± 0.254

7.411 ± 0.444

9.929 ± 0.55

9.406 ± 0.464

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.275

5.891 ± 0.314

2.304 ± 0.269

4.323 ± 0.499

4.727 ± 0.371

5.558 ± 0.363

5.938 ± 0.355

5.606 ± 0.464

0.831 ± 0.108

4.133 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski