Aphanomyces stellatus

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae; Aphanomyces

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A485KTB5|A0A485KTB5_9STRA Aste57867_11406 protein OS=Aphanomyces stellatus OX=120398 GN=Aste57867_11406 PE=4 SV=1
MM1 pKa = 7.3NALHH5 pKa = 6.81HH6 pKa = 7.03ADD8 pKa = 3.82CAANGAPALDD18 pKa = 3.99EE19 pKa = 5.06DD20 pKa = 4.35EE21 pKa = 5.27QILATFSRR29 pKa = 11.84TQMYY33 pKa = 8.61TNTYY37 pKa = 10.1GPDD40 pKa = 3.33EE41 pKa = 5.29DD42 pKa = 6.07DD43 pKa = 3.75NGTMEE48 pKa = 4.27HH49 pKa = 6.59KK50 pKa = 10.73GVGEE54 pKa = 4.31LYY56 pKa = 9.55VTTTRR61 pKa = 11.84IAWVFGTNWEE71 pKa = 4.13HH72 pKa = 7.55DD73 pKa = 3.42AGAVVGYY80 pKa = 9.71AWDD83 pKa = 3.5MTYY86 pKa = 11.29LSLHH90 pKa = 6.68AISRR94 pKa = 11.84DD95 pKa = 2.9ISSFRR100 pKa = 11.84FPCLYY105 pKa = 10.39CQLDD109 pKa = 3.64VEE111 pKa = 5.1DD112 pKa = 4.64EE113 pKa = 4.29VNEE116 pKa = 3.89IRR118 pKa = 11.84FVPQEE123 pKa = 4.07PEE125 pKa = 3.96KK126 pKa = 10.48EE127 pKa = 4.04LQAMFDD133 pKa = 3.77AFSASAALNPDD144 pKa = 3.72EE145 pKa = 6.44DD146 pKa = 6.22DD147 pKa = 4.6DD148 pKa = 6.78DD149 pKa = 4.88EE150 pKa = 4.89PQGDD154 pKa = 4.1WIYY157 pKa = 11.37NADD160 pKa = 3.87EE161 pKa = 4.43VEE163 pKa = 4.31SGARR167 pKa = 11.84EE168 pKa = 4.04AQMAAHH174 pKa = 7.12FDD176 pKa = 4.33SILHH180 pKa = 5.7VSPALAGGVAGQFDD194 pKa = 4.25DD195 pKa = 6.26ADD197 pKa = 4.85DD198 pKa = 4.73EE199 pKa = 5.24DD200 pKa = 6.48DD201 pKa = 3.97EE202 pKa = 5.3LLL204 pKa = 4.64

Molecular weight: 22.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A485LFX1|A0A485LFX1_9STRA Aste57867_20183 protein OS=Aphanomyces stellatus OX=120398 GN=Aste57867_20183 PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 10.43GLAATLLAFVLCIVLFTAQANEE24 pKa = 4.12SGLANHH30 pKa = 6.63HH31 pKa = 5.34QRR33 pKa = 11.84AVEE36 pKa = 3.97GNAMVEE42 pKa = 4.47EE43 pKa = 4.35LRR45 pKa = 11.84QLTRR49 pKa = 11.84KK50 pKa = 5.7TTAAPASSTTNTKK63 pKa = 8.6ATPTTTRR70 pKa = 11.84SSAAPAPSTTRR81 pKa = 11.84SAAPATTKK89 pKa = 9.59KK90 pKa = 6.45TTKK93 pKa = 10.25KK94 pKa = 9.44VAKK97 pKa = 9.73KK98 pKa = 9.4VAKK101 pKa = 8.88KK102 pKa = 6.8TTTKK106 pKa = 10.35TPAKK110 pKa = 9.19KK111 pKa = 6.98TTKK114 pKa = 10.25KK115 pKa = 9.56VAKK118 pKa = 9.55KK119 pKa = 10.12KK120 pKa = 10.06AVKK123 pKa = 8.83KK124 pKa = 6.1TTSAPTKK131 pKa = 10.45KK132 pKa = 8.64PTKK135 pKa = 8.78KK136 pKa = 8.59TAVKK140 pKa = 10.44KK141 pKa = 10.28DD142 pKa = 3.43VKK144 pKa = 10.5KK145 pKa = 10.83SKK147 pKa = 10.75KK148 pKa = 7.69PTKK151 pKa = 10.36KK152 pKa = 8.81PTKK155 pKa = 10.08KK156 pKa = 8.66PTKK159 pKa = 9.56KK160 pKa = 7.13TTTKK164 pKa = 10.84KK165 pKa = 10.35NVKK168 pKa = 9.56KK169 pKa = 10.64SKK171 pKa = 10.65KK172 pKa = 7.39PTKK175 pKa = 10.36KK176 pKa = 8.81PTKK179 pKa = 10.08KK180 pKa = 8.66PTKK183 pKa = 9.56KK184 pKa = 7.13TTTKK188 pKa = 10.84KK189 pKa = 10.35NVKK192 pKa = 9.56KK193 pKa = 10.64SKK195 pKa = 10.65KK196 pKa = 7.39PTKK199 pKa = 10.36KK200 pKa = 8.81PTKK203 pKa = 10.2KK204 pKa = 8.77PTKK207 pKa = 9.04KK208 pKa = 8.92TGKK211 pKa = 8.56KK212 pKa = 4.29TTKK215 pKa = 8.84KK216 pKa = 8.69TAIKK220 pKa = 9.16TADD223 pKa = 3.39KK224 pKa = 10.71KK225 pKa = 11.08AATEE229 pKa = 3.68PRR231 pKa = 11.84RR232 pKa = 11.84RR233 pKa = 11.84TTRR236 pKa = 11.84KK237 pKa = 8.72PSKK240 pKa = 9.61NGKK243 pKa = 8.78KK244 pKa = 9.73AVSRR248 pKa = 11.84RR249 pKa = 11.84RR250 pKa = 11.84TTRR253 pKa = 11.84KK254 pKa = 8.81PSKK257 pKa = 9.61NGKK260 pKa = 8.78KK261 pKa = 9.73AVSRR265 pKa = 11.84RR266 pKa = 11.84RR267 pKa = 11.84TTRR270 pKa = 11.84KK271 pKa = 8.81PSKK274 pKa = 9.51NGKK277 pKa = 8.92KK278 pKa = 10.14AVISRR283 pKa = 11.84RR284 pKa = 11.84TTRR287 pKa = 11.84KK288 pKa = 7.64PLKK291 pKa = 8.67YY292 pKa = 9.87GKK294 pKa = 9.88KK295 pKa = 9.04AVIRR299 pKa = 11.84RR300 pKa = 11.84RR301 pKa = 11.84TTRR304 pKa = 11.84KK305 pKa = 8.83PLKK308 pKa = 9.38NGKK311 pKa = 9.17KK312 pKa = 8.94AVSRR316 pKa = 11.84RR317 pKa = 11.84RR318 pKa = 11.84TTRR321 pKa = 11.84KK322 pKa = 8.81PSKK325 pKa = 9.89NGKK328 pKa = 7.52KK329 pKa = 8.0TVIRR333 pKa = 11.84RR334 pKa = 11.84RR335 pKa = 11.84TTRR338 pKa = 11.84KK339 pKa = 8.83PLKK342 pKa = 9.38NGKK345 pKa = 9.17KK346 pKa = 8.94AVSRR350 pKa = 11.84RR351 pKa = 11.84RR352 pKa = 11.84TTRR355 pKa = 11.84KK356 pKa = 8.81PSKK359 pKa = 9.61NGKK362 pKa = 8.78KK363 pKa = 9.73AVSRR367 pKa = 11.84RR368 pKa = 11.84RR369 pKa = 11.84TTRR372 pKa = 11.84KK373 pKa = 8.81PSKK376 pKa = 9.61NGKK379 pKa = 8.78KK380 pKa = 9.73AVSRR384 pKa = 11.84RR385 pKa = 11.84RR386 pKa = 11.84TTRR389 pKa = 11.84KK390 pKa = 8.81PSKK393 pKa = 9.59NGKK396 pKa = 8.43KK397 pKa = 10.06ASIRR401 pKa = 11.84RR402 pKa = 11.84RR403 pKa = 11.84TTRR406 pKa = 11.84KK407 pKa = 9.07PSKK410 pKa = 9.57KK411 pKa = 9.56GKK413 pKa = 9.19KK414 pKa = 8.61AVSRR418 pKa = 11.84RR419 pKa = 11.84RR420 pKa = 11.84TTRR423 pKa = 11.84KK424 pKa = 8.81PSKK427 pKa = 9.61NGKK430 pKa = 8.78KK431 pKa = 9.73AVSRR435 pKa = 11.84RR436 pKa = 11.84RR437 pKa = 11.84TTRR440 pKa = 11.84KK441 pKa = 8.81PSKK444 pKa = 9.59NGKK447 pKa = 8.43KK448 pKa = 10.06ASIRR452 pKa = 11.84RR453 pKa = 11.84RR454 pKa = 11.84TTRR457 pKa = 11.84KK458 pKa = 9.07PSKK461 pKa = 9.57KK462 pKa = 9.56GKK464 pKa = 9.19KK465 pKa = 8.61AVSRR469 pKa = 11.84RR470 pKa = 11.84RR471 pKa = 11.84TTRR474 pKa = 11.84KK475 pKa = 8.81PSKK478 pKa = 9.61NGKK481 pKa = 8.78KK482 pKa = 9.73AVSRR486 pKa = 11.84RR487 pKa = 11.84RR488 pKa = 11.84TTRR491 pKa = 11.84KK492 pKa = 8.81PSKK495 pKa = 9.61NGKK498 pKa = 8.78KK499 pKa = 9.73AVSRR503 pKa = 11.84RR504 pKa = 11.84RR505 pKa = 11.84TTRR508 pKa = 11.84KK509 pKa = 8.81PSKK512 pKa = 9.88NGKK515 pKa = 9.22KK516 pKa = 9.83AVKK519 pKa = 9.75KK520 pKa = 10.39PSGKK524 pKa = 6.21TTKK527 pKa = 10.2KK528 pKa = 9.85PSKK531 pKa = 7.85TTTKK535 pKa = 10.25KK536 pKa = 10.48PVVTTSAPAAGQNATSGASAAVAVVNANN564 pKa = 3.09

Molecular weight: 62.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20738

0

20738

9730605

66

13490

469.2

51.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.822 ± 0.021

1.862 ± 0.01

5.878 ± 0.015

4.892 ± 0.02

3.898 ± 0.012

5.726 ± 0.037

3.059 ± 0.013

4.223 ± 0.011

4.482 ± 0.019

9.75 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.008

3.385 ± 0.012

5.337 ± 0.019

3.982 ± 0.011

5.503 ± 0.018

7.22 ± 0.02

6.618 ± 0.021

7.633 ± 0.016

1.446 ± 0.006

2.66 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski