Aeromonas phage Ah1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4YEY4|A0A2H4YEY4_9CAUD Baseplate tail tube initiator OS=Aeromonas phage Ah1 OX=2053701 GN=54_2 PE=4 SV=1
MM1 pKa = 7.52NIDD4 pKa = 3.64NLFDD8 pKa = 4.5FMDD11 pKa = 4.53ALRR14 pKa = 11.84KK15 pKa = 9.38QDD17 pKa = 3.54AAEE20 pKa = 4.59DD21 pKa = 4.72FIDD24 pKa = 4.74SDD26 pKa = 4.88GIDD29 pKa = 3.26TALEE33 pKa = 3.89EE34 pKa = 4.37VVNYY38 pKa = 10.19EE39 pKa = 4.35LGKK42 pKa = 8.88PSHH45 pKa = 6.62GSRR48 pKa = 11.84HH49 pKa = 5.77FYY51 pKa = 10.02GCGYY55 pKa = 9.72KK56 pKa = 10.18LDD58 pKa = 4.23MNWITVYY65 pKa = 10.83YY66 pKa = 9.74EE67 pKa = 4.14QGCSCCNDD75 pKa = 3.33KK76 pKa = 10.98EE77 pKa = 4.57DD78 pKa = 5.05YY79 pKa = 10.62SISYY83 pKa = 9.15QKK85 pKa = 11.05LWDD88 pKa = 3.97HH89 pKa = 6.19LVKK92 pKa = 10.16TNRR95 pKa = 11.84VVEE98 pKa = 4.09IEE100 pKa = 4.13EE101 pKa = 4.03

Molecular weight: 11.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4YFN2|A0A2H4YFN2_9CAUD Uncharacterized protein OS=Aeromonas phage Ah1 OX=2053701 GN=Ah1_00286 PE=4 SV=1
MM1 pKa = 7.88NDD3 pKa = 3.06TTKK6 pKa = 10.75LARR9 pKa = 11.84LMKK12 pKa = 10.3DD13 pKa = 3.18VEE15 pKa = 3.93RR16 pKa = 11.84HH17 pKa = 3.75ARR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84QRR23 pKa = 11.84RR24 pKa = 11.84SGVIQPRR31 pKa = 11.84MSMDD35 pKa = 3.67ALSKK39 pKa = 10.58LLKK42 pKa = 9.63QANEE46 pKa = 4.08RR47 pKa = 11.84TKK49 pKa = 10.94LLYY52 pKa = 10.43SEE54 pKa = 4.8LTCC57 pKa = 5.84

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

332

0

332

66624

29

1385

200.7

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.639 ± 0.151

1.234 ± 0.071

6.462 ± 0.104

6.805 ± 0.166

4.338 ± 0.085

6.342 ± 0.164

1.954 ± 0.074

6.768 ± 0.113

7.646 ± 0.16

7.014 ± 0.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.16 ± 0.096

5.47 ± 0.134

3.142 ± 0.07

3.349 ± 0.085

5.06 ± 0.1

6.055 ± 0.098

5.747 ± 0.195

7.364 ± 0.116

1.295 ± 0.047

4.159 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski