Amino acid dipepetide frequency for Aeromonas phage Ah1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.833AlaAla: 4.833 ± 0.433
0.766AlaCys: 0.766 ± 0.119
4.023AlaAsp: 4.023 ± 0.273
4.308AlaGlu: 4.308 ± 0.29
2.972AlaPhe: 2.972 ± 0.224
4.938AlaGly: 4.938 ± 0.383
1.081AlaHis: 1.081 ± 0.121
4.743AlaIle: 4.743 ± 0.258
5.238AlaLys: 5.238 ± 0.293
4.518AlaLeu: 4.518 ± 0.266
2.312AlaMet: 2.312 ± 0.167
3.602AlaAsn: 3.602 ± 0.3
1.786AlaPro: 1.786 ± 0.173
2.417AlaGln: 2.417 ± 0.213
3.317AlaArg: 3.317 ± 0.257
3.467AlaSer: 3.467 ± 0.248
4.098AlaThr: 4.098 ± 0.369
4.488AlaVal: 4.488 ± 0.269
0.946AlaTrp: 0.946 ± 0.118
2.537AlaTyr: 2.537 ± 0.158
0.0AlaXaa: 0.0 ± 0.0
Cys
0.615CysAla: 0.615 ± 0.092
0.12CysCys: 0.12 ± 0.041
0.811CysAsp: 0.811 ± 0.093
0.826CysGlu: 0.826 ± 0.113
0.525CysPhe: 0.525 ± 0.084
0.811CysGly: 0.811 ± 0.121
0.3CysHis: 0.3 ± 0.068
0.841CysIle: 0.841 ± 0.116
1.111CysLys: 1.111 ± 0.146
0.766CysLeu: 0.766 ± 0.114
0.195CysMet: 0.195 ± 0.05
0.57CysAsn: 0.57 ± 0.1
0.54CysPro: 0.54 ± 0.109
0.255CysGln: 0.255 ± 0.066
0.871CysArg: 0.871 ± 0.108
0.916CysSer: 0.916 ± 0.126
0.555CysThr: 0.555 ± 0.095
0.931CysVal: 0.931 ± 0.138
0.165CysTrp: 0.165 ± 0.048
0.615CysTyr: 0.615 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
4.053AspAla: 4.053 ± 0.255
0.766AspCys: 0.766 ± 0.115
3.722AspAsp: 3.722 ± 0.298
4.188AspGlu: 4.188 ± 0.235
3.302AspPhe: 3.302 ± 0.237
5.043AspGly: 5.043 ± 0.293
1.216AspHis: 1.216 ± 0.156
4.668AspIle: 4.668 ± 0.248
4.188AspLys: 4.188 ± 0.268
4.743AspLeu: 4.743 ± 0.285
2.026AspMet: 2.026 ± 0.183
3.092AspAsn: 3.092 ± 0.212
2.026AspPro: 2.026 ± 0.181
1.771AspGln: 1.771 ± 0.181
2.897AspArg: 2.897 ± 0.199
3.963AspSer: 3.963 ± 0.266
3.242AspThr: 3.242 ± 0.305
4.938AspVal: 4.938 ± 0.265
1.201AspTrp: 1.201 ± 0.13
3.572AspTyr: 3.572 ± 0.268
0.0AspXaa: 0.0 ± 0.0
Glu
4.443GluAla: 4.443 ± 0.271
0.916GluCys: 0.916 ± 0.12
3.587GluAsp: 3.587 ± 0.249
4.158GluGlu: 4.158 ± 0.307
3.677GluPhe: 3.677 ± 0.262
2.927GluGly: 2.927 ± 0.212
1.396GluHis: 1.396 ± 0.14
5.328GluIle: 5.328 ± 0.319
4.188GluLys: 4.188 ± 0.303
6.694GluLeu: 6.694 ± 0.284
2.402GluMet: 2.402 ± 0.191
3.542GluAsn: 3.542 ± 0.236
1.381GluPro: 1.381 ± 0.143
2.987GluGln: 2.987 ± 0.245
3.632GluArg: 3.632 ± 0.222
3.933GluSer: 3.933 ± 0.273
4.038GluThr: 4.038 ± 0.239
4.608GluVal: 4.608 ± 0.293
0.766GluTrp: 0.766 ± 0.108
3.452GluTyr: 3.452 ± 0.209
0.0GluXaa: 0.0 ± 0.0
Phe
3.062PheAla: 3.062 ± 0.205
0.57PheCys: 0.57 ± 0.09
3.647PheAsp: 3.647 ± 0.227
3.227PheGlu: 3.227 ± 0.261
1.456PhePhe: 1.456 ± 0.163
3.122PheGly: 3.122 ± 0.235
0.75PheHis: 0.75 ± 0.135
2.492PheIle: 2.492 ± 0.215
3.287PheLys: 3.287 ± 0.24
2.492PheLeu: 2.492 ± 0.205
1.591PheMet: 1.591 ± 0.186
2.567PheAsn: 2.567 ± 0.205
1.081PhePro: 1.081 ± 0.143
1.066PheGln: 1.066 ± 0.122
1.996PheArg: 1.996 ± 0.153
2.477PheSer: 2.477 ± 0.194
2.672PheThr: 2.672 ± 0.186
3.347PheVal: 3.347 ± 0.232
0.465PheTrp: 0.465 ± 0.082
2.011PheTyr: 2.011 ± 0.184
0.0PheXaa: 0.0 ± 0.0
Gly
4.143GlyAla: 4.143 ± 0.323
0.826GlyCys: 0.826 ± 0.133
3.963GlyAsp: 3.963 ± 0.358
4.218GlyGlu: 4.218 ± 0.233
2.777GlyPhe: 2.777 ± 0.217
3.557GlyGly: 3.557 ± 0.35
1.036GlyHis: 1.036 ± 0.128
4.563GlyIle: 4.563 ± 0.273
5.659GlyLys: 5.659 ± 0.32
4.008GlyLeu: 4.008 ± 0.232
1.966GlyMet: 1.966 ± 0.21
4.053GlyAsn: 4.053 ± 0.358
1.096GlyPro: 1.096 ± 0.134
2.086GlyGln: 2.086 ± 0.188
2.837GlyArg: 2.837 ± 0.186
3.963GlySer: 3.963 ± 0.36
4.068GlyThr: 4.068 ± 0.336
4.653GlyVal: 4.653 ± 0.288
0.946GlyTrp: 0.946 ± 0.103
3.002GlyTyr: 3.002 ± 0.2
0.0GlyXaa: 0.0 ± 0.0
His
1.051HisAla: 1.051 ± 0.105
0.315HisCys: 0.315 ± 0.071
1.156HisAsp: 1.156 ± 0.126
1.441HisGlu: 1.441 ± 0.157
0.901HisPhe: 0.901 ± 0.122
1.621HisGly: 1.621 ± 0.188
0.45HisHis: 0.45 ± 0.082
1.141HisIle: 1.141 ± 0.145
1.516HisLys: 1.516 ± 0.147
1.501HisLeu: 1.501 ± 0.148
0.525HisMet: 0.525 ± 0.094
1.066HisAsn: 1.066 ± 0.133
0.811HisPro: 0.811 ± 0.121
0.615HisGln: 0.615 ± 0.087
0.856HisArg: 0.856 ± 0.118
1.021HisSer: 1.021 ± 0.122
1.096HisThr: 1.096 ± 0.129
1.201HisVal: 1.201 ± 0.162
0.405HisTrp: 0.405 ± 0.094
0.856HisTyr: 0.856 ± 0.139
0.0HisXaa: 0.0 ± 0.0
Ile
5.013IleAla: 5.013 ± 0.288
0.856IleCys: 0.856 ± 0.133
4.818IleAsp: 4.818 ± 0.263
5.794IleGlu: 5.794 ± 0.33
1.681IlePhe: 1.681 ± 0.139
4.593IleGly: 4.593 ± 0.259
1.276IleHis: 1.276 ± 0.138
3.903IleIle: 3.903 ± 0.25
5.569IleLys: 5.569 ± 0.309
3.647IleLeu: 3.647 ± 0.229
2.206IleMet: 2.206 ± 0.205
3.587IleAsn: 3.587 ± 0.245
2.477IlePro: 2.477 ± 0.176
2.372IleGln: 2.372 ± 0.201
4.008IleArg: 4.008 ± 0.205
4.008IleSer: 4.008 ± 0.251
3.993IleThr: 3.993 ± 0.282
4.878IleVal: 4.878 ± 0.321
0.585IleTrp: 0.585 ± 0.104
2.417IleTyr: 2.417 ± 0.18
0.0IleXaa: 0.0 ± 0.0
Lys
5.238LysAla: 5.238 ± 0.306
1.066LysCys: 1.066 ± 0.142
5.088LysAsp: 5.088 ± 0.258
5.343LysGlu: 5.343 ± 0.314
3.692LysPhe: 3.692 ± 0.242
3.257LysGly: 3.257 ± 0.29
1.816LysHis: 1.816 ± 0.218
4.998LysIle: 4.998 ± 0.26
5.449LysLys: 5.449 ± 0.393
5.779LysLeu: 5.779 ± 0.324
2.522LysMet: 2.522 ± 0.235
4.383LysAsn: 4.383 ± 0.262
2.266LysPro: 2.266 ± 0.192
3.092LysGln: 3.092 ± 0.28
4.203LysArg: 4.203 ± 0.303
4.953LysSer: 4.953 ± 0.271
4.323LysThr: 4.323 ± 0.284
5.028LysVal: 5.028 ± 0.305
1.096LysTrp: 1.096 ± 0.136
2.867LysTyr: 2.867 ± 0.2
0.0LysXaa: 0.0 ± 0.0
Leu
4.698LeuAla: 4.698 ± 0.35
0.991LeuCys: 0.991 ± 0.148
4.833LeuAsp: 4.833 ± 0.276
4.848LeuGlu: 4.848 ± 0.329
2.507LeuPhe: 2.507 ± 0.187
4.593LeuGly: 4.593 ± 0.314
1.456LeuHis: 1.456 ± 0.158
4.638LeuIle: 4.638 ± 0.246
6.064LeuLys: 6.064 ± 0.353
4.623LeuLeu: 4.623 ± 0.282
1.756LeuMet: 1.756 ± 0.193
3.918LeuAsn: 3.918 ± 0.322
2.822LeuPro: 2.822 ± 0.233
2.086LeuGln: 2.086 ± 0.172
3.347LeuArg: 3.347 ± 0.236
4.383LeuSer: 4.383 ± 0.27
4.143LeuThr: 4.143 ± 0.29
5.133LeuVal: 5.133 ± 0.257
0.69LeuTrp: 0.69 ± 0.109
2.612LeuTyr: 2.612 ± 0.201
0.0LeuXaa: 0.0 ± 0.0
Met
1.816MetAla: 1.816 ± 0.14
0.3MetCys: 0.3 ± 0.072
1.321MetAsp: 1.321 ± 0.142
1.816MetGlu: 1.816 ± 0.185
1.696MetPhe: 1.696 ± 0.174
1.486MetGly: 1.486 ± 0.169
0.766MetHis: 0.766 ± 0.108
2.567MetIle: 2.567 ± 0.195
3.077MetLys: 3.077 ± 0.226
2.687MetLeu: 2.687 ± 0.208
0.886MetMet: 0.886 ± 0.101
2.206MetAsn: 2.206 ± 0.241
0.976MetPro: 0.976 ± 0.116
1.021MetGln: 1.021 ± 0.128
1.336MetArg: 1.336 ± 0.149
2.462MetSer: 2.462 ± 0.202
2.221MetThr: 2.221 ± 0.196
1.606MetVal: 1.606 ± 0.146
0.3MetTrp: 0.3 ± 0.066
1.051MetTyr: 1.051 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
4.068AsnAla: 4.068 ± 0.265
0.675AsnCys: 0.675 ± 0.095
2.882AsnAsp: 2.882 ± 0.258
3.497AsnGlu: 3.497 ± 0.197
2.312AsnPhe: 2.312 ± 0.217
4.488AsnGly: 4.488 ± 0.269
1.066AsnHis: 1.066 ± 0.133
3.347AsnIle: 3.347 ± 0.238
4.248AsnLys: 4.248 ± 0.296
4.128AsnLeu: 4.128 ± 0.301
1.711AsnMet: 1.711 ± 0.158
2.717AsnAsn: 2.717 ± 0.231
2.447AsnPro: 2.447 ± 0.223
1.741AsnGln: 1.741 ± 0.17
2.777AsnArg: 2.777 ± 0.226
3.062AsnSer: 3.062 ± 0.251
2.867AsnThr: 2.867 ± 0.267
4.413AsnVal: 4.413 ± 0.291
0.69AsnTrp: 0.69 ± 0.11
1.561AsnTyr: 1.561 ± 0.152
0.0AsnXaa: 0.0 ± 0.0
Pro
2.206ProAla: 2.206 ± 0.172
0.345ProCys: 0.345 ± 0.061
2.342ProAsp: 2.342 ± 0.178
2.176ProGlu: 2.176 ± 0.192
1.456ProPhe: 1.456 ± 0.148
1.786ProGly: 1.786 ± 0.198
0.495ProHis: 0.495 ± 0.075
1.996ProIle: 1.996 ± 0.162
2.627ProLys: 2.627 ± 0.2
1.636ProLeu: 1.636 ± 0.143
0.946ProMet: 0.946 ± 0.137
1.636ProAsn: 1.636 ± 0.134
0.69ProPro: 0.69 ± 0.106
0.946ProGln: 0.946 ± 0.135
1.426ProArg: 1.426 ± 0.131
1.966ProSer: 1.966 ± 0.167
2.206ProThr: 2.206 ± 0.179
2.912ProVal: 2.912 ± 0.187
0.48ProTrp: 0.48 ± 0.078
1.141ProTyr: 1.141 ± 0.122
0.0ProXaa: 0.0 ± 0.0
Gln
2.552GlnAla: 2.552 ± 0.202
0.36GlnCys: 0.36 ± 0.068
1.876GlnAsp: 1.876 ± 0.186
2.266GlnGlu: 2.266 ± 0.207
1.471GlnPhe: 1.471 ± 0.17
1.786GlnGly: 1.786 ± 0.172
0.675GlnHis: 0.675 ± 0.097
2.612GlnIle: 2.612 ± 0.197
2.176GlnLys: 2.176 ± 0.211
2.281GlnLeu: 2.281 ± 0.205
1.411GlnMet: 1.411 ± 0.143
1.666GlnAsn: 1.666 ± 0.165
1.096GlnPro: 1.096 ± 0.128
1.306GlnGln: 1.306 ± 0.157
1.951GlnArg: 1.951 ± 0.197
1.936GlnSer: 1.936 ± 0.171
1.816GlnThr: 1.816 ± 0.155
2.417GlnVal: 2.417 ± 0.188
0.51GlnTrp: 0.51 ± 0.096
1.321GlnTyr: 1.321 ± 0.141
0.0GlnXaa: 0.0 ± 0.0
Arg
2.972ArgAla: 2.972 ± 0.228
0.48ArgCys: 0.48 ± 0.092
3.257ArgAsp: 3.257 ± 0.205
3.347ArgGlu: 3.347 ± 0.247
2.191ArgPhe: 2.191 ± 0.193
3.062ArgGly: 3.062 ± 0.207
1.126ArgHis: 1.126 ± 0.143
3.722ArgIle: 3.722 ± 0.285
3.602ArgLys: 3.602 ± 0.237
3.587ArgLeu: 3.587 ± 0.202
2.026ArgMet: 2.026 ± 0.183
2.612ArgAsn: 2.612 ± 0.169
1.531ArgPro: 1.531 ± 0.146
1.816ArgGln: 1.816 ± 0.176
2.522ArgArg: 2.522 ± 0.215
2.867ArgSer: 2.867 ± 0.198
2.251ArgThr: 2.251 ± 0.176
3.963ArgVal: 3.963 ± 0.234
0.916ArgTrp: 0.916 ± 0.124
2.747ArgTyr: 2.747 ± 0.261
0.0ArgXaa: 0.0 ± 0.0
Ser
3.452SerAla: 3.452 ± 0.289
0.6SerCys: 0.6 ± 0.104
4.128SerAsp: 4.128 ± 0.233
4.218SerGlu: 4.218 ± 0.257
2.972SerPhe: 2.972 ± 0.211
4.203SerGly: 4.203 ± 0.285
1.036SerHis: 1.036 ± 0.132
3.557SerIle: 3.557 ± 0.264
4.743SerLys: 4.743 ± 0.268
4.083SerLeu: 4.083 ± 0.25
2.101SerMet: 2.101 ± 0.18
3.377SerAsn: 3.377 ± 0.267
1.936SerPro: 1.936 ± 0.181
1.816SerGln: 1.816 ± 0.178
2.987SerArg: 2.987 ± 0.232
3.993SerSer: 3.993 ± 0.271
3.542SerThr: 3.542 ± 0.266
4.728SerVal: 4.728 ± 0.307
0.735SerTrp: 0.735 ± 0.11
2.342SerTyr: 2.342 ± 0.188
0.0SerXaa: 0.0 ± 0.0
Thr
3.782ThrAla: 3.782 ± 0.245
0.675ThrCys: 0.675 ± 0.102
3.767ThrAsp: 3.767 ± 0.308
3.662ThrGlu: 3.662 ± 0.257
2.387ThrPhe: 2.387 ± 0.172
4.413ThrGly: 4.413 ± 0.448
1.231ThrHis: 1.231 ± 0.14
3.948ThrIle: 3.948 ± 0.259
4.278ThrLys: 4.278 ± 0.25
4.233ThrLeu: 4.233 ± 0.263
1.471ThrMet: 1.471 ± 0.183
2.792ThrAsn: 2.792 ± 0.31
2.312ThrPro: 2.312 ± 0.243
1.651ThrGln: 1.651 ± 0.167
3.017ThrArg: 3.017 ± 0.229
3.287ThrSer: 3.287 ± 0.27
2.987ThrThr: 2.987 ± 0.295
4.773ThrVal: 4.773 ± 0.331
0.6ThrTrp: 0.6 ± 0.121
2.206ThrTyr: 2.206 ± 0.203
0.0ThrXaa: 0.0 ± 0.0
Val
4.818ValAla: 4.818 ± 0.28
0.901ValCys: 0.901 ± 0.119
5.404ValAsp: 5.404 ± 0.285
5.359ValGlu: 5.359 ± 0.301
3.152ValPhe: 3.152 ± 0.218
4.278ValGly: 4.278 ± 0.272
1.216ValHis: 1.216 ± 0.156
4.848ValIle: 4.848 ± 0.296
5.419ValLys: 5.419 ± 0.284
4.923ValLeu: 4.923 ± 0.279
1.951ValMet: 1.951 ± 0.168
4.278ValAsn: 4.278 ± 0.256
2.492ValPro: 2.492 ± 0.202
2.221ValGln: 2.221 ± 0.222
3.722ValArg: 3.722 ± 0.237
4.803ValSer: 4.803 ± 0.226
4.503ValThr: 4.503 ± 0.29
5.494ValVal: 5.494 ± 0.334
0.735ValTrp: 0.735 ± 0.129
3.107ValTyr: 3.107 ± 0.254
0.0ValXaa: 0.0 ± 0.0
Trp
0.826TrpAla: 0.826 ± 0.145
0.21TrpCys: 0.21 ± 0.055
1.081TrpAsp: 1.081 ± 0.127
1.021TrpGlu: 1.021 ± 0.125
0.72TrpPhe: 0.72 ± 0.095
0.51TrpGly: 0.51 ± 0.094
0.33TrpHis: 0.33 ± 0.07
0.946TrpIle: 0.946 ± 0.121
1.201TrpLys: 1.201 ± 0.139
0.886TrpLeu: 0.886 ± 0.12
0.435TrpMet: 0.435 ± 0.083
0.72TrpAsn: 0.72 ± 0.097
0.135TrpPro: 0.135 ± 0.043
0.54TrpGln: 0.54 ± 0.091
0.585TrpArg: 0.585 ± 0.093
0.645TrpSer: 0.645 ± 0.097
0.525TrpThr: 0.525 ± 0.096
0.826TrpVal: 0.826 ± 0.118
0.165TrpTrp: 0.165 ± 0.05
0.645TrpTyr: 0.645 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.747TyrAla: 2.747 ± 0.17
0.6TyrCys: 0.6 ± 0.093
2.912TyrAsp: 2.912 ± 0.231
2.342TyrGlu: 2.342 ± 0.193
1.531TyrPhe: 1.531 ± 0.154
2.942TyrGly: 2.942 ± 0.22
0.826TyrHis: 0.826 ± 0.1
2.822TyrIle: 2.822 ± 0.195
2.822TyrLys: 2.822 ± 0.225
2.927TyrLeu: 2.927 ± 0.207
1.141TyrMet: 1.141 ± 0.126
2.312TyrAsn: 2.312 ± 0.224
1.516TyrPro: 1.516 ± 0.152
1.681TyrGln: 1.681 ± 0.15
2.312TyrArg: 2.312 ± 0.209
2.447TyrSer: 2.447 ± 0.179
2.327TyrThr: 2.327 ± 0.218
3.302TyrVal: 3.302 ± 0.285
0.555TyrTrp: 0.555 ± 0.072
1.531TyrTyr: 1.531 ± 0.153
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 332 proteins (66624 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski