Blueberry latent virus
Average proteome isoelectric point is 8.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D1D8L7|D1D8L7_9VIRU ORF1 OS=Blueberry latent virus OX=430710 PE=4 SV=1
MM1 pKa = 7.66 AGASGSILPVEE12 pKa = 4.53 TTEE15 pKa = 3.86 QTQARR20 pKa = 11.84 VSTALAPLTGVLGNIEE36 pKa = 4.36 RR37 pKa = 11.84 FSVEE41 pKa = 4.54 AIINSNLTVDD51 pKa = 4.22 IFLKK55 pKa = 10.66 AIKK58 pKa = 10.41 SLVAYY63 pKa = 8.55 AQQDD67 pKa = 3.29 ILEE70 pKa = 5.05 NIASAANTAGAIQHH84 pKa = 6.25 VINMTPKK91 pKa = 10.74 DD92 pKa = 4.03 LFDD95 pKa = 4.26 LSRR98 pKa = 11.84 WVKK101 pKa = 8.75 TAAGMEE107 pKa = 4.72 AISKK111 pKa = 8.38 ITTQRR116 pKa = 11.84 RR117 pKa = 11.84 LLRR120 pKa = 11.84 QGGAGRR126 pKa = 11.84 SASQVAWVNLFTQQQADD143 pKa = 3.72 YY144 pKa = 9.9 AQEE147 pKa = 4.49 KK148 pKa = 8.31 KK149 pKa = 10.77 RR150 pKa = 11.84 KK151 pKa = 4.9 MTRR154 pKa = 11.84 FEE156 pKa = 4.48 RR157 pKa = 11.84 KK158 pKa = 9.47 RR159 pKa = 11.84 EE160 pKa = 3.94 DD161 pKa = 3.94 LKK163 pKa = 11.55 LQLAQLDD170 pKa = 3.95 TDD172 pKa = 3.97 EE173 pKa = 4.53 AASMEE178 pKa = 4.23 RR179 pKa = 11.84 LAAKK183 pKa = 10.49 YY184 pKa = 6.87 PTQVALPATMEE195 pKa = 4.28 TEE197 pKa = 4.48 LVAACWAAYY206 pKa = 10.04 VADD209 pKa = 4.1 CDD211 pKa = 4.02 RR212 pKa = 11.84 RR213 pKa = 11.84 GITPSAKK220 pKa = 9.39 TNASLTEE227 pKa = 4.02 AVKK230 pKa = 10.39 HH231 pKa = 5.0 YY232 pKa = 9.93 SANVRR237 pKa = 11.84 DD238 pKa = 4.35 QILTTYY244 pKa = 10.46 CEE246 pKa = 4.19 QEE248 pKa = 4.16 NVQADD253 pKa = 3.88 LTRR256 pKa = 11.84 YY257 pKa = 9.62 ARR259 pKa = 11.84 EE260 pKa = 4.22 KK261 pKa = 9.76 IQSFRR266 pKa = 11.84 DD267 pKa = 3.25 SGNQGGVTRR276 pKa = 11.84 VRR278 pKa = 11.84 NLYY281 pKa = 8.73 TAATGAAIDD290 pKa = 4.1 IPPPRR295 pKa = 11.84 AQEE298 pKa = 4.32 VPHH301 pKa = 5.63 EE302 pKa = 4.41 TGPVRR307 pKa = 11.84 DD308 pKa = 5.31 PIAQTGEE315 pKa = 3.91 AQLQAVAHH323 pKa = 5.26 QALTGVVIEE332 pKa = 4.94 ADD334 pKa = 3.38 PDD336 pKa = 3.72 RR337 pKa = 11.84 AQGEE341 pKa = 4.32 ASSGDD346 pKa = 3.54 SDD348 pKa = 3.68 RR349 pKa = 11.84 ASAASTEE356 pKa = 4.42 STRR359 pKa = 11.84 RR360 pKa = 11.84 LQPDD364 pKa = 3.07 RR365 pKa = 11.84 RR366 pKa = 11.84 VKK368 pKa = 10.54 KK369 pKa = 10.11 PRR371 pKa = 11.84 TTGRR375 pKa = 3.22
Molecular weight: 40.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.541
IPC2_protein 7.819
IPC_protein 7.863
Toseland 7.922
ProMoST 8.507
Dawson 8.551
Bjellqvist 8.668
Wikipedia 8.536
Rodwell 8.58
Grimsley 7.746
Solomon 8.77
Lehninger 8.756
Nozaki 8.814
DTASelect 8.507
Thurlkill 8.595
EMBOSS 8.756
Sillero 8.843
Patrickios 4.851
IPC_peptide 8.756
IPC2_peptide 7.497
IPC2.peptide.svr19 7.63
Protein with the highest isoelectric point:
>tr|D1D8L7|D1D8L7_9VIRU ORF1 OS=Blueberry latent virus OX=430710 PE=4 SV=1
MM1 pKa = 7.66 AGASGSILPVEE12 pKa = 4.53 TTEE15 pKa = 3.86 QTQARR20 pKa = 11.84 VSTALAPLTGVLGNIEE36 pKa = 4.36 RR37 pKa = 11.84 FSVEE41 pKa = 4.54 AIINSNLTVDD51 pKa = 4.22 IFLKK55 pKa = 10.66 AIKK58 pKa = 10.41 SLVAYY63 pKa = 8.55 AQQDD67 pKa = 3.29 ILEE70 pKa = 5.05 NIASAANTAGAIQHH84 pKa = 6.25 VINMTPKK91 pKa = 10.74 DD92 pKa = 4.03 LFDD95 pKa = 4.26 LSRR98 pKa = 11.84 WVKK101 pKa = 8.75 TAAGMEE107 pKa = 4.72 AISKK111 pKa = 8.38 ITTQRR116 pKa = 11.84 RR117 pKa = 11.84 LLRR120 pKa = 11.84 QGGAGRR126 pKa = 11.84 SASQVAWVNLFTQQQADD143 pKa = 3.72 YY144 pKa = 9.9 AQEE147 pKa = 4.49 KK148 pKa = 8.31 KK149 pKa = 10.77 RR150 pKa = 11.84 KK151 pKa = 4.9 MTRR154 pKa = 11.84 FEE156 pKa = 4.48 RR157 pKa = 11.84 KK158 pKa = 9.47 RR159 pKa = 11.84 EE160 pKa = 3.94 DD161 pKa = 3.94 LKK163 pKa = 11.55 LQLAQLDD170 pKa = 3.95 TDD172 pKa = 3.97 EE173 pKa = 4.53 AASMEE178 pKa = 4.23 RR179 pKa = 11.84 LAAKK183 pKa = 10.49 YY184 pKa = 6.87 PTQVALPATMEE195 pKa = 4.28 TEE197 pKa = 4.48 LVAACWAAYY206 pKa = 10.04 VADD209 pKa = 4.1 CDD211 pKa = 4.02 RR212 pKa = 11.84 RR213 pKa = 11.84 GITPSAKK220 pKa = 9.39 TNASLTEE227 pKa = 4.02 AVKK230 pKa = 10.39 HH231 pKa = 5.0 YY232 pKa = 9.93 SANVRR237 pKa = 11.84 DD238 pKa = 4.35 QILTTYY244 pKa = 10.46 CEE246 pKa = 4.19 QEE248 pKa = 4.16 NVQADD253 pKa = 3.88 LTRR256 pKa = 11.84 YY257 pKa = 9.62 ARR259 pKa = 11.84 EE260 pKa = 4.22 KK261 pKa = 9.76 IQSFRR266 pKa = 11.84 DD267 pKa = 3.22 SGNQEE272 pKa = 3.83 EE273 pKa = 5.02 SPVLEE278 pKa = 3.71 ISIRR282 pKa = 11.84 QQLEE286 pKa = 3.44 QRR288 pKa = 11.84 LISLPLEE295 pKa = 3.72 LRR297 pKa = 11.84 KK298 pKa = 9.56 FHH300 pKa = 6.23 MKK302 pKa = 9.46 QVPFGIPLHH311 pKa = 5.86 KK312 pKa = 9.89 RR313 pKa = 11.84 GRR315 pKa = 11.84 PSCKK319 pKa = 9.47 PLLTKK324 pKa = 10.81 LSPEE328 pKa = 3.76 LLLRR332 pKa = 11.84 RR333 pKa = 11.84 TQIGHH338 pKa = 5.99 RR339 pKa = 11.84 EE340 pKa = 3.93 KK341 pKa = 10.61 PPLGIRR347 pKa = 11.84 TEE349 pKa = 4.27 LAQPPLKK356 pKa = 10.68 ALEE359 pKa = 4.45 DD360 pKa = 3.84 CSRR363 pKa = 11.84 IGVLRR368 pKa = 11.84 SLAPQADD375 pKa = 3.54 KK376 pKa = 11.16 LIPRR380 pKa = 11.84 SRR382 pKa = 11.84 SKK384 pKa = 10.47 WEE386 pKa = 3.71 AGVRR390 pKa = 11.84 KK391 pKa = 9.86 IIGGGEE397 pKa = 3.78 LKK399 pKa = 10.69 DD400 pKa = 3.3 WFKK403 pKa = 11.45 AKK405 pKa = 10.43 SMYY408 pKa = 10.28 RR409 pKa = 11.84 GGGNLFDD416 pKa = 4.73 ALRR419 pKa = 11.84 LLSACDD425 pKa = 4.44 DD426 pKa = 3.76 YY427 pKa = 12.06 DD428 pKa = 4.5 GYY430 pKa = 9.88 TRR432 pKa = 11.84 LKK434 pKa = 10.61 GNYY437 pKa = 7.94 SVEE440 pKa = 3.97 AARR443 pKa = 11.84 EE444 pKa = 3.99 KK445 pKa = 11.03 LLLPSGLRR453 pKa = 11.84 VPDD456 pKa = 3.76 GVGCVHH462 pKa = 6.7 MKK464 pKa = 10.44 NFNDD468 pKa = 3.75 EE469 pKa = 4.0 ASAGPLCRR477 pKa = 11.84 AFGIRR482 pKa = 11.84 RR483 pKa = 11.84 KK484 pKa = 10.3 AGLKK488 pKa = 10.13 SRR490 pKa = 11.84 LEE492 pKa = 3.76 QFAWEE497 pKa = 4.35 IYY499 pKa = 10.51 DD500 pKa = 4.39 GIGNGDD506 pKa = 4.43 LDD508 pKa = 4.92 LGAIPPFLARR518 pKa = 11.84 VGYY521 pKa = 7.7 RR522 pKa = 11.84 TKK524 pKa = 10.93 LVTGEE529 pKa = 3.54 KK530 pKa = 10.46 AMRR533 pKa = 11.84 KK534 pKa = 9.19 ISTGDD539 pKa = 3.42 SIGRR543 pKa = 11.84 AIMMLDD549 pKa = 3.38 AYY551 pKa = 8.96 EE552 pKa = 4.1 QAFSSPIFNVISDD565 pKa = 4.33 AVTHH569 pKa = 6.16 LHH571 pKa = 6.87 SDD573 pKa = 3.51 PTSGWRR579 pKa = 11.84 NYY581 pKa = 10.47 LIRR584 pKa = 11.84 ASSDD588 pKa = 2.41 WGKK591 pKa = 9.81 MYY593 pKa = 11.07 KK594 pKa = 9.73 EE595 pKa = 3.68 MRR597 pKa = 11.84 KK598 pKa = 9.92 AKK600 pKa = 10.44 VVVEE604 pKa = 4.07 LDD606 pKa = 2.85 WKK608 pKa = 10.89 KK609 pKa = 10.72 FDD611 pKa = 3.81 RR612 pKa = 11.84 EE613 pKa = 3.9 RR614 pKa = 11.84 PADD617 pKa = 4.26 HH618 pKa = 6.97 ISFFIDD624 pKa = 3.93 VILSCFAPRR633 pKa = 11.84 TTRR636 pKa = 11.84 EE637 pKa = 3.79 EE638 pKa = 4.25 KK639 pKa = 10.96 LLGAYY644 pKa = 8.84 RR645 pKa = 11.84 RR646 pKa = 11.84 AMEE649 pKa = 4.06 NALLHH654 pKa = 6.1 RR655 pKa = 11.84 VLVTDD660 pKa = 4.23 DD661 pKa = 4.3 GGVLTVDD668 pKa = 3.62 GMVPSGSLWTGIIGTGLNILYY689 pKa = 9.92 IGYY692 pKa = 9.5 ALSDD696 pKa = 3.33 IGISPLNYY704 pKa = 9.42 VPKK707 pKa = 10.68 CAGDD711 pKa = 3.85 DD712 pKa = 3.48 NLTFFSRR719 pKa = 11.84 DD720 pKa = 3.29 YY721 pKa = 11.43 GDD723 pKa = 4.02 AAFKK727 pKa = 10.57 RR728 pKa = 11.84 LRR730 pKa = 11.84 VKK732 pKa = 10.62 LNEE735 pKa = 3.97 MFRR738 pKa = 11.84 ANIDD742 pKa = 3.42 EE743 pKa = 4.22 EE744 pKa = 4.61 DD745 pKa = 4.58 FIIHH749 pKa = 6.76 RR750 pKa = 11.84 PPFFVTKK757 pKa = 9.76 AQAVFPPGTDD767 pKa = 3.19 LSKK770 pKa = 10.48 GTSSILNQCKK780 pKa = 8.03 WVQFEE785 pKa = 4.36 GEE787 pKa = 4.24 IIIDD791 pKa = 3.53 EE792 pKa = 4.39 AMGLSHH798 pKa = 6.75 RR799 pKa = 11.84 WEE801 pKa = 3.9 YY802 pKa = 11.05 RR803 pKa = 11.84 FQGKK807 pKa = 8.83 PKK809 pKa = 10.29 FLANYY814 pKa = 7.33 WLPDD818 pKa = 3.23 GRR820 pKa = 11.84 SIRR823 pKa = 11.84 PATDD827 pKa = 2.85 SLEE830 pKa = 3.94 RR831 pKa = 11.84 LLYY834 pKa = 10.2 PEE836 pKa = 5.48 GLHH839 pKa = 5.74 QTIEE843 pKa = 4.28 DD844 pKa = 3.8 YY845 pKa = 11.61 EE846 pKa = 4.37 MAVLAMVVDD855 pKa = 4.46 NPWNSHH861 pKa = 4.71 NVNHH865 pKa = 5.89 MMHH868 pKa = 7.24 RR869 pKa = 11.84 FCIVQQIKK877 pKa = 8.69 RR878 pKa = 11.84 QSMVGIKK885 pKa = 10.4 AEE887 pKa = 3.92 DD888 pKa = 3.55 VMWYY892 pKa = 10.21 SKK894 pKa = 11.02 LRR896 pKa = 11.84 GKK898 pKa = 10.17 EE899 pKa = 4.08 GEE901 pKa = 4.55 GIPYY905 pKa = 8.99 PQVAYY910 pKa = 9.51 WRR912 pKa = 11.84 RR913 pKa = 11.84 QEE915 pKa = 4.27 KK916 pKa = 10.11 KK917 pKa = 10.1 VFMEE921 pKa = 5.15 EE922 pKa = 3.55 IPEE925 pKa = 3.86 LMGYY929 pKa = 9.07 IKK931 pKa = 10.65 KK932 pKa = 9.86 FQTFLSGVSTLYY944 pKa = 11.02 SRR946 pKa = 11.84 DD947 pKa = 3.37 SAGGIDD953 pKa = 3.19 AWMFMSILRR962 pKa = 11.84 GEE964 pKa = 4.29 RR965 pKa = 11.84 DD966 pKa = 3.05 VGAGQFGNDD975 pKa = 3.05 IIEE978 pKa = 3.96 WCKK981 pKa = 10.34 FLNKK985 pKa = 10.12 NPLTSGLRR993 pKa = 11.84 AAKK996 pKa = 10.09 RR997 pKa = 11.84 FRR999 pKa = 11.84 GEE1001 pKa = 3.83 VRR1003 pKa = 11.84 SEE1005 pKa = 3.83 EE1006 pKa = 4.46 ASTQVAQRR1014 pKa = 11.84 WVEE1017 pKa = 3.97 MISTVRR1023 pKa = 11.84 EE1024 pKa = 3.87 RR1025 pKa = 11.84 LRR1027 pKa = 11.84 NPLLGSPRR1035 pKa = 11.84 SFALWVSDD1043 pKa = 3.39 MYY1045 pKa = 11.63 AEE1047 pKa = 4.26 NVFSNVSS1054 pKa = 3.02
Molecular weight: 118.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.503
IPC2_protein 8.404
IPC_protein 8.287
Toseland 8.887
ProMoST 8.843
Dawson 9.253
Bjellqvist 9.151
Wikipedia 9.414
Rodwell 9.428
Grimsley 9.268
Solomon 9.297
Lehninger 9.253
Nozaki 9.268
DTASelect 9.033
Thurlkill 9.151
EMBOSS 9.399
Sillero 9.326
Patrickios 4.825
IPC_peptide 9.297
IPC2_peptide 7.805
IPC2.peptide.svr19 7.681
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1429
375
1054
714.5
79.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.357 ± 2.341
1.12 ± 0.164
5.318 ± 0.008
6.298 ± 0.084
3.289 ± 0.864
6.648 ± 0.673
1.4 ± 0.17
5.808 ± 0.379
5.388 ± 0.574
8.957 ± 0.899
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.519 ± 0.47
3.359 ± 0.081
4.269 ± 0.137
4.899 ± 1.177
7.838 ± 0.083
6.578 ± 0.227
5.738 ± 1.566
5.878 ± 0.267
1.61 ± 0.414
2.729 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here