Muribaculaceae bacterium Isolate-080 (Janvier)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; unclassified Muribaculaceae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2KZQ2|A0A3N2KZQ2_9BACT DUF5063 domain-containing protein OS=Muribaculaceae bacterium Isolate-080 (Janvier) OX=2486468 GN=EEL39_01620 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 6.19LSPNSLAFIALCNEE16 pKa = 4.02FCYY19 pKa = 10.37AVEE22 pKa = 4.11NARR25 pKa = 11.84EE26 pKa = 4.16STRR29 pKa = 11.84NAFVASMLRR38 pKa = 11.84LLPRR42 pKa = 11.84IYY44 pKa = 10.35ISASDD49 pKa = 4.61LKK51 pKa = 10.68PEE53 pKa = 4.13PLIDD57 pKa = 4.05EE58 pKa = 4.71DD59 pKa = 3.91DD60 pKa = 4.32AYY62 pKa = 10.17IEE64 pKa = 4.41SFLDD68 pKa = 3.15EE69 pKa = 5.34DD70 pKa = 4.73YY71 pKa = 10.97YY72 pKa = 11.35EE73 pKa = 4.48SMRR76 pKa = 11.84RR77 pKa = 11.84QIEE80 pKa = 4.18NLLGADD86 pKa = 3.67DD87 pKa = 4.51VYY89 pKa = 11.77LEE91 pKa = 4.23VFEE94 pKa = 5.74EE95 pKa = 4.05DD96 pKa = 3.32MKK98 pKa = 11.72YY99 pKa = 10.84SDD101 pKa = 4.15TPIGMSVAEE110 pKa = 4.92GICDD114 pKa = 3.6VFQVLYY120 pKa = 11.06NFIVTVKK127 pKa = 9.88DD128 pKa = 3.5APSEE132 pKa = 4.28VVNAALTAVRR142 pKa = 11.84DD143 pKa = 4.01DD144 pKa = 4.42FGSYY148 pKa = 7.94WSQRR152 pKa = 11.84VCNLMRR158 pKa = 11.84PLNHH162 pKa = 7.02LFYY165 pKa = 11.14SSDD168 pKa = 3.58NDD170 pKa = 4.21DD171 pKa = 4.75DD172 pKa = 6.15DD173 pKa = 5.86NII175 pKa = 4.89

Molecular weight: 20.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2KJY6|A0A3N2KJY6_9BACT Uncharacterized protein OS=Muribaculaceae bacterium Isolate-080 (Janvier) OX=2486468 GN=EEL39_14570 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.4AEE4 pKa = 4.12LSIVITFIFSLFFWFLRR21 pKa = 11.84QNSAINQRR29 pKa = 11.84GFNSSLRR36 pKa = 11.84GSRR39 pKa = 11.84KK40 pKa = 9.07IFMDD44 pKa = 3.45NGSVTRR50 pKa = 11.84TDD52 pKa = 3.55GATSCLLAGNGACSWRR68 pKa = 11.84LRR70 pKa = 11.84YY71 pKa = 9.9LCGKK75 pKa = 9.6LL76 pKa = 3.39

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3287

0

3287

1130632

23

3087

344.0

38.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.703 ± 0.036

1.234 ± 0.018

6.096 ± 0.029

6.272 ± 0.041

4.296 ± 0.027

6.904 ± 0.04

1.896 ± 0.019

6.984 ± 0.04

5.782 ± 0.039

8.675 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.018

4.914 ± 0.031

4.063 ± 0.027

2.91 ± 0.026

5.417 ± 0.036

6.615 ± 0.035

5.733 ± 0.03

6.467 ± 0.039

1.211 ± 0.019

4.074 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski