Streptococcus satellite phage Javan729

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZV24|A0A4D5ZV24_9VIRU DNA primase OS=Streptococcus satellite phage Javan729 OX=2558823 GN=JavanS729_0020 PE=4 SV=1
MM1 pKa = 7.77GYY3 pKa = 9.4MIGTYY8 pKa = 7.78EE9 pKa = 4.23VPSISITYY17 pKa = 9.21KK18 pKa = 10.51NKK20 pKa = 7.46EE21 pKa = 4.3TLVAEE26 pKa = 4.14YY27 pKa = 11.24VEE29 pKa = 4.69DD30 pKa = 3.79KK31 pKa = 10.51KK32 pKa = 11.45LNYY35 pKa = 9.01IFKK38 pKa = 10.26RR39 pKa = 11.84ICDD42 pKa = 3.78SFKK45 pKa = 10.87DD46 pKa = 4.25YY47 pKa = 11.42YY48 pKa = 9.81KK49 pKa = 10.78QPVNDD54 pKa = 3.4EE55 pKa = 4.25AFEE58 pKa = 3.94EE59 pKa = 4.95RR60 pKa = 11.84YY61 pKa = 9.8KK62 pKa = 10.7RR63 pKa = 11.84EE64 pKa = 3.48RR65 pKa = 11.84VLQLKK70 pKa = 9.9EE71 pKa = 4.29SEE73 pKa = 4.49DD74 pKa = 4.18FEE76 pKa = 5.17SLLNKK81 pKa = 8.18VTDD84 pKa = 3.93YY85 pKa = 11.19EE86 pKa = 5.2LEE88 pKa = 4.08LLDD91 pKa = 3.66YY92 pKa = 11.22AEE94 pKa = 5.43RR95 pKa = 11.84LLSDD99 pKa = 4.27KK100 pKa = 10.25PILMNSMTAFGTLEE114 pKa = 3.8MLGNEE119 pKa = 5.07SINLFQKK126 pKa = 10.8LDD128 pKa = 3.41VEE130 pKa = 4.73GEE132 pKa = 4.36YY133 pKa = 10.49KK134 pKa = 10.78GLADD138 pKa = 4.73YY139 pKa = 11.02SGQQ142 pKa = 3.43

Molecular weight: 16.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZYZ5|A0A4D5ZYZ5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan729 OX=2558823 GN=JavanS729_0016 PE=4 SV=1
MM1 pKa = 7.38GKK3 pKa = 9.59SATNMKK9 pKa = 10.18RR10 pKa = 11.84IATIFLLGLPKK21 pKa = 9.96VPKK24 pKa = 8.97MRR26 pKa = 11.84PIAYY30 pKa = 7.67HH31 pKa = 7.13APQCSTILKK40 pKa = 9.4RR41 pKa = 11.84VLSRR45 pKa = 11.84ASASPLTLSKK55 pKa = 10.48PFSFLGCC62 pKa = 4.16

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

4032

39

507

155.1

17.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.456 ± 0.344

0.893 ± 0.237

5.06 ± 0.331

8.309 ± 0.518

3.77 ± 0.385

5.655 ± 0.407

1.687 ± 0.255

7.292 ± 0.336

9.623 ± 0.6

10.243 ± 0.579

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.009 ± 0.209

5.382 ± 0.395

3.125 ± 0.343

4.092 ± 0.3

5.407 ± 0.357

5.754 ± 0.331

6.324 ± 0.398

4.911 ± 0.467

0.769 ± 0.138

4.241 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski