Candidatus Deianiraea vastatrix
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1126 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B8XI47|A0A5B8XI47_9RICK 50S ribosomal protein L35 OS=Candidatus Deianiraea vastatrix OX=2163644 GN=Deia_00623 PE=4 SV=1
MM1 pKa = 7.49 IWRR4 pKa = 11.84 EE5 pKa = 4.02 MFNFSQDD12 pKa = 3.25 CVLMVLASDD21 pKa = 4.76 YY22 pKa = 10.7 YY23 pKa = 11.25 DD24 pKa = 3.26 EE25 pKa = 4.77 SEE27 pKa = 4.15 YY28 pKa = 10.55 VRR30 pKa = 11.84 NYY32 pKa = 10.99 EE33 pKa = 4.28 EE34 pKa = 4.27 FLKK37 pKa = 10.97 LVNVV41 pKa = 4.28
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.938
IPC2_protein 4.228
IPC_protein 3.986
Toseland 3.834
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.834
Grimsley 3.757
Solomon 3.923
Lehninger 3.884
Nozaki 4.101
DTASelect 4.19
Thurlkill 3.884
EMBOSS 3.884
Sillero 4.101
Patrickios 0.604
IPC_peptide 3.935
IPC2_peptide 4.088
IPC2.peptide.svr19 4.02
Protein with the highest isoelectric point:
>tr|A0A5B8XD90|A0A5B8XD90_9RICK Tetratricopeptide repeat protein OS=Candidatus Deianiraea vastatrix OX=2163644 GN=Deia_00544 PE=4 SV=1
MM1 pKa = 7.46 LRR3 pKa = 11.84 TYY5 pKa = 10.51 QPSKK9 pKa = 9.22 RR10 pKa = 11.84 KK11 pKa = 9.44 RR12 pKa = 11.84 RR13 pKa = 11.84 NSHH16 pKa = 5.08 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 9.96 GGRR28 pKa = 11.84 KK29 pKa = 8.65 VLSNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.36 GRR39 pKa = 11.84 HH40 pKa = 5.57 SIAII44 pKa = 3.9
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1126
0
1126
365583
30
3323
324.7
36.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.887 ± 0.069
1.519 ± 0.032
5.779 ± 0.06
5.938 ± 0.077
5.178 ± 0.064
5.832 ± 0.087
1.675 ± 0.029
10.119 ± 0.111
9.01 ± 0.084
8.248 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.57 ± 0.036
6.685 ± 0.093
2.731 ± 0.043
3.557 ± 0.102
3.324 ± 0.054
7.42 ± 0.074
4.699 ± 0.063
5.511 ± 0.07
0.599 ± 0.018
3.718 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here