Domibacillus aminovorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Domibacillus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177L6X3|A0A177L6X3_9BACI Urease subunit gamma OS=Domibacillus aminovorans OX=29332 GN=ureA PE=3 SV=1
MM1 pKa = 7.64AKK3 pKa = 8.95YY4 pKa = 9.47TMIDD8 pKa = 3.21KK9 pKa = 7.61EE10 pKa = 4.53TCIACGACGAAAPDD24 pKa = 3.33IYY26 pKa = 10.99DD27 pKa = 3.84YY28 pKa = 11.45DD29 pKa = 4.3DD30 pKa = 3.72EE31 pKa = 7.04GIAFVKK37 pKa = 10.7LDD39 pKa = 3.85DD40 pKa = 4.06NEE42 pKa = 4.57GVVEE46 pKa = 4.34VPEE49 pKa = 4.13MLHH52 pKa = 6.93EE53 pKa = 4.47DD54 pKa = 3.77MIDD57 pKa = 3.44AFEE60 pKa = 4.53GCPTDD65 pKa = 3.71SIKK68 pKa = 10.9VAEE71 pKa = 4.52EE72 pKa = 4.15PFDD75 pKa = 4.41GDD77 pKa = 3.69ANKK80 pKa = 10.29FEE82 pKa = 4.36

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177KQ38|A0A177KQ38_9BACI 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase OS=Domibacillus aminovorans OX=29332 GN=hisA PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.72RR12 pKa = 11.84SKK14 pKa = 9.38VHH16 pKa = 5.82GFRR19 pKa = 11.84TRR21 pKa = 11.84MSSKK25 pKa = 9.72NGRR28 pKa = 11.84RR29 pKa = 11.84VIASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.63GRR39 pKa = 11.84KK40 pKa = 8.74VLSAA44 pKa = 4.05

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3573

0

3573

1010879

27

2046

282.9

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.667 ± 0.047

0.722 ± 0.013

5.204 ± 0.03

7.556 ± 0.043

4.467 ± 0.035

6.983 ± 0.038

2.124 ± 0.022

7.625 ± 0.037

6.627 ± 0.037

9.459 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.98 ± 0.022

4.047 ± 0.029

3.647 ± 0.024

3.577 ± 0.026

4.296 ± 0.029

5.92 ± 0.026

5.614 ± 0.028

7.138 ± 0.035

1.004 ± 0.014

3.345 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski