Celeribacter ethanolicus
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3982 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A291GAA1|A0A291GAA1_9RHOB Amino acid ABC transporter substrate-binding protein OS=Celeribacter ethanolicus OX=1758178 GN=CEW89_05760 PE=3 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 LPVLTALTAMLSLPALADD20 pKa = 3.82 PLPSYY25 pKa = 9.26 WQDD28 pKa = 3.56 DD29 pKa = 3.37 RR30 pKa = 11.84 DD31 pKa = 4.39 FYY33 pKa = 12.08 NEE35 pKa = 3.91 IADD38 pKa = 4.02 AACVGSADD46 pKa = 5.11 DD47 pKa = 4.28 SDD49 pKa = 4.04 FDD51 pKa = 4.57 RR52 pKa = 11.84 LITAAIQEE60 pKa = 4.36 ANAPAMIALRR70 pKa = 11.84 WMVLSEE76 pKa = 4.5 SCVQYY81 pKa = 11.32 NPDD84 pKa = 4.05 GDD86 pKa = 5.08 LLTNSLMVMAALQAYY101 pKa = 7.84 PIAQSNLAGSYY112 pKa = 8.77 LHH114 pKa = 7.17 GYY116 pKa = 9.12 YY117 pKa = 10.59 GIEE120 pKa = 4.01 QNPDD124 pKa = 3.01 EE125 pKa = 5.78 AEE127 pKa = 3.89 ALYY130 pKa = 10.65 DD131 pKa = 3.46 QAIQGGYY138 pKa = 10.14 GEE140 pKa = 4.85 AASEE144 pKa = 4.06 YY145 pKa = 10.71 ARR147 pKa = 11.84 ALIEE151 pKa = 4.3 GDD153 pKa = 3.09 ILTRR157 pKa = 11.84 DD158 pKa = 3.44 LDD160 pKa = 3.76 KK161 pKa = 11.35 AADD164 pKa = 3.95 LLAVAEE170 pKa = 4.31 RR171 pKa = 11.84 EE172 pKa = 4.37 GVEE175 pKa = 4.09 AATLAPLRR183 pKa = 11.84 TRR185 pKa = 11.84 LSEE188 pKa = 4.1 LGGAEE193 pKa = 3.82 VGEE196 pKa = 4.1 PHH198 pKa = 7.43 IDD200 pKa = 3.77 PSWDD204 pKa = 3.46 GVDD207 pKa = 3.41 TRR209 pKa = 11.84 RR210 pKa = 11.84 LAEE213 pKa = 3.7 WLYY216 pKa = 10.33 EE217 pKa = 3.9 GLYY220 pKa = 11.04 GSDD223 pKa = 3.43 DD224 pKa = 3.81 TEE226 pKa = 3.95
Molecular weight: 24.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.617
ProMoST 3.961
Dawson 3.808
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.139
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.935
Patrickios 1.163
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|A0A291GFR2|A0A291GFR2_9RHOB Carboxymuconolactone decarboxylase OS=Celeribacter ethanolicus OX=1758178 GN=CEW89_15515 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 AGRR28 pKa = 11.84 KK29 pKa = 8.46 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3982
0
3982
1216105
38
2900
305.4
33.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.721 ± 0.048
0.857 ± 0.014
5.906 ± 0.035
6.393 ± 0.041
3.847 ± 0.026
8.548 ± 0.038
2.067 ± 0.021
5.245 ± 0.027
3.585 ± 0.033
10.102 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.891 ± 0.018
2.553 ± 0.022
5.011 ± 0.03
3.028 ± 0.021
6.379 ± 0.04
5.307 ± 0.03
5.631 ± 0.036
7.237 ± 0.034
1.352 ± 0.018
2.342 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here