Faecalibacterium phage FP_Lugh

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lughvirus; Faecalibacterium virus Lugh

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9V3K1|A0A2K9V3K1_9CAUD Minor capsid protein OS=Faecalibacterium phage FP_Lugh OX=2070184 PE=4 SV=1
MM1 pKa = 7.69ASYY4 pKa = 10.53LISDD8 pKa = 4.31APYY11 pKa = 10.45APWLSEE17 pKa = 3.79VLATLEE23 pKa = 3.75EE24 pKa = 4.2HH25 pKa = 6.7KK26 pKa = 10.18IDD28 pKa = 5.23RR29 pKa = 11.84IAIAAPLADD38 pKa = 3.45GEE40 pKa = 4.78VFTGYY45 pKa = 11.41YY46 pKa = 10.63NMNTQDD52 pKa = 3.61KK53 pKa = 10.56ALLASNIQADD63 pKa = 3.77AVLDD67 pKa = 4.1AVCHH71 pKa = 5.45NGQRR75 pKa = 11.84IQQAWEE81 pKa = 3.98DD82 pKa = 4.06DD83 pKa = 3.99EE84 pKa = 5.34EE85 pKa = 4.65GG86 pKa = 3.54

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9V3C3|A0A2K9V3C3_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_Lugh OX=2070184 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.05IKK4 pKa = 10.73SRR6 pKa = 11.84VWYY9 pKa = 8.68WLAAASGAVSLLYY22 pKa = 11.1GMGIEE27 pKa = 5.24GGAQLGSSISDD38 pKa = 3.6SQFVTALCLVLAAVAFLRR56 pKa = 11.84LGFAAQDD63 pKa = 3.38RR64 pKa = 11.84EE65 pKa = 4.39QNARR69 pKa = 11.84RR70 pKa = 11.84YY71 pKa = 9.65GRR73 pKa = 11.84VDD75 pKa = 3.1RR76 pKa = 11.84THH78 pKa = 6.94ARR80 pKa = 11.84TEE82 pKa = 4.06EE83 pKa = 3.61PDD85 pKa = 3.18YY86 pKa = 10.7RR87 pKa = 11.84QNRR90 pKa = 11.84RR91 pKa = 11.84GAA93 pKa = 3.37

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

10738

61

1084

255.7

28.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.473 ± 0.58

1.276 ± 0.194

6.342 ± 0.214

6.64 ± 0.293

2.989 ± 0.231

7.273 ± 0.279

1.714 ± 0.237

4.47 ± 0.204

5.634 ± 0.28

8.856 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.142

3.222 ± 0.187

4.228 ± 0.395

4.563 ± 0.3

5.737 ± 0.406

5.802 ± 0.405

6.696 ± 0.521

5.886 ± 0.255

1.63 ± 0.154

3.138 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski