Amino acid dipepetide frequency for Faecalibacterium phage FP_Lugh

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.764AlaAla: 16.764 ± 2.649
1.304AlaCys: 1.304 ± 0.345
9.034AlaAsp: 9.034 ± 0.715
9.314AlaGlu: 9.314 ± 1.358
3.446AlaPhe: 3.446 ± 0.679
7.171AlaGly: 7.171 ± 1.165
1.49AlaHis: 1.49 ± 0.345
4.936AlaIle: 4.936 ± 0.716
6.706AlaLys: 6.706 ± 0.838
10.617AlaLeu: 10.617 ± 0.946
2.608AlaMet: 2.608 ± 0.369
3.539AlaAsn: 3.539 ± 0.874
4.936AlaPro: 4.936 ± 0.995
6.24AlaGln: 6.24 ± 0.843
5.495AlaArg: 5.495 ± 0.736
5.868AlaSer: 5.868 ± 1.308
4.471AlaThr: 4.471 ± 0.829
6.24AlaVal: 6.24 ± 0.646
1.676AlaTrp: 1.676 ± 0.279
2.887AlaTyr: 2.887 ± 0.481
0.0AlaXaa: 0.0 ± 0.0
Cys
1.583CysAla: 1.583 ± 0.519
0.186CysCys: 0.186 ± 0.117
0.652CysAsp: 0.652 ± 0.245
0.745CysGlu: 0.745 ± 0.23
0.466CysPhe: 0.466 ± 0.239
1.118CysGly: 1.118 ± 0.265
0.559CysHis: 0.559 ± 0.234
0.373CysIle: 0.373 ± 0.152
0.838CysLys: 0.838 ± 0.283
1.211CysLeu: 1.211 ± 0.306
0.093CysMet: 0.093 ± 0.093
0.559CysAsn: 0.559 ± 0.223
0.466CysPro: 0.466 ± 0.222
0.373CysGln: 0.373 ± 0.162
0.838CysArg: 0.838 ± 0.297
0.745CysSer: 0.745 ± 0.295
0.745CysThr: 0.745 ± 0.21
0.559CysVal: 0.559 ± 0.251
0.279CysTrp: 0.279 ± 0.204
0.373CysTyr: 0.373 ± 0.197
0.0CysXaa: 0.0 ± 0.0
Asp
7.265AspAla: 7.265 ± 0.956
0.931AspCys: 0.931 ± 0.294
3.26AspAsp: 3.26 ± 0.501
4.564AspGlu: 4.564 ± 0.509
2.235AspPhe: 2.235 ± 0.445
5.588AspGly: 5.588 ± 0.992
0.559AspHis: 0.559 ± 0.237
2.701AspIle: 2.701 ± 0.396
3.539AspLys: 3.539 ± 0.647
5.122AspLeu: 5.122 ± 0.579
1.583AspMet: 1.583 ± 0.4
1.397AspAsn: 1.397 ± 0.357
3.819AspPro: 3.819 ± 0.661
1.676AspGln: 1.676 ± 0.301
3.912AspArg: 3.912 ± 0.545
3.446AspSer: 3.446 ± 0.65
3.446AspThr: 3.446 ± 0.561
4.377AspVal: 4.377 ± 0.712
0.838AspTrp: 0.838 ± 0.257
3.073AspTyr: 3.073 ± 0.457
0.0AspXaa: 0.0 ± 0.0
Glu
8.475GluAla: 8.475 ± 0.989
0.838GluCys: 0.838 ± 0.307
4.005GluAsp: 4.005 ± 0.562
4.377GluGlu: 4.377 ± 0.665
1.956GluPhe: 1.956 ± 0.487
4.471GluGly: 4.471 ± 0.664
0.931GluHis: 0.931 ± 0.342
2.422GluIle: 2.422 ± 0.435
4.098GluLys: 4.098 ± 0.633
6.054GluLeu: 6.054 ± 0.742
1.956GluMet: 1.956 ± 0.364
3.073GluAsn: 3.073 ± 0.586
2.422GluPro: 2.422 ± 0.592
4.936GluGln: 4.936 ± 0.849
3.353GluArg: 3.353 ± 0.502
3.632GluSer: 3.632 ± 0.602
2.701GluThr: 2.701 ± 0.549
3.632GluVal: 3.632 ± 0.616
1.211GluTrp: 1.211 ± 0.326
1.863GluTyr: 1.863 ± 0.43
0.0GluXaa: 0.0 ± 0.0
Phe
2.608PheAla: 2.608 ± 0.455
0.838PheCys: 0.838 ± 0.359
2.515PheAsp: 2.515 ± 0.516
1.956PheGlu: 1.956 ± 0.459
1.118PhePhe: 1.118 ± 0.373
2.701PheGly: 2.701 ± 0.428
0.466PheHis: 0.466 ± 0.202
1.583PheIle: 1.583 ± 0.395
0.745PheLys: 0.745 ± 0.246
2.049PheLeu: 2.049 ± 0.481
1.118PheMet: 1.118 ± 0.34
0.466PheAsn: 0.466 ± 0.188
0.838PhePro: 0.838 ± 0.291
1.49PheGln: 1.49 ± 0.318
1.956PheArg: 1.956 ± 0.415
2.235PheSer: 2.235 ± 0.454
1.676PheThr: 1.676 ± 0.342
1.77PheVal: 1.77 ± 0.401
0.931PheTrp: 0.931 ± 0.296
0.838PheTyr: 0.838 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
6.892GlyAla: 6.892 ± 0.746
0.931GlyCys: 0.931 ± 0.374
4.098GlyAsp: 4.098 ± 0.57
3.167GlyGlu: 3.167 ± 0.514
2.235GlyPhe: 2.235 ± 0.497
5.495GlyGly: 5.495 ± 1.018
1.304GlyHis: 1.304 ± 0.365
3.446GlyIle: 3.446 ± 0.519
5.495GlyLys: 5.495 ± 0.703
5.681GlyLeu: 5.681 ± 0.651
2.422GlyMet: 2.422 ± 0.47
1.77GlyAsn: 1.77 ± 0.302
1.211GlyPro: 1.211 ± 0.347
3.26GlyGln: 3.26 ± 0.438
4.936GlyArg: 4.936 ± 0.651
5.495GlySer: 5.495 ± 0.879
5.588GlyThr: 5.588 ± 0.825
4.564GlyVal: 4.564 ± 0.613
1.77GlyTrp: 1.77 ± 0.434
2.98GlyTyr: 2.98 ± 0.513
0.0GlyXaa: 0.0 ± 0.0
His
1.583HisAla: 1.583 ± 0.476
0.186HisCys: 0.186 ± 0.132
1.304HisAsp: 1.304 ± 0.379
0.838HisGlu: 0.838 ± 0.271
0.745HisPhe: 0.745 ± 0.252
1.304HisGly: 1.304 ± 0.375
0.652HisHis: 0.652 ± 0.264
0.838HisIle: 0.838 ± 0.306
0.931HisLys: 0.931 ± 0.332
1.118HisLeu: 1.118 ± 0.317
0.279HisMet: 0.279 ± 0.165
0.931HisAsn: 0.931 ± 0.29
0.652HisPro: 0.652 ± 0.257
0.0HisGln: 0.0 ± 0.0
0.838HisArg: 0.838 ± 0.219
1.77HisSer: 1.77 ± 0.909
1.397HisThr: 1.397 ± 0.499
1.118HisVal: 1.118 ± 0.353
0.373HisTrp: 0.373 ± 0.17
0.279HisTyr: 0.279 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
4.005IleAla: 4.005 ± 0.471
0.931IleCys: 0.931 ± 0.29
4.284IleAsp: 4.284 ± 0.575
3.167IleGlu: 3.167 ± 0.48
1.211IlePhe: 1.211 ± 0.316
3.073IleGly: 3.073 ± 0.536
1.304IleHis: 1.304 ± 0.345
1.956IleIle: 1.956 ± 0.429
1.304IleLys: 1.304 ± 0.321
3.632IleLeu: 3.632 ± 0.445
0.931IleMet: 0.931 ± 0.255
1.397IleAsn: 1.397 ± 0.394
2.142IlePro: 2.142 ± 0.434
2.049IleGln: 2.049 ± 0.457
3.073IleArg: 3.073 ± 0.528
2.794IleSer: 2.794 ± 0.611
3.26IleThr: 3.26 ± 0.6
2.515IleVal: 2.515 ± 0.427
0.466IleTrp: 0.466 ± 0.199
1.211IleTyr: 1.211 ± 0.301
0.0IleXaa: 0.0 ± 0.0
Lys
8.662LysAla: 8.662 ± 0.991
0.0LysCys: 0.0 ± 0.0
3.446LysAsp: 3.446 ± 0.46
4.005LysGlu: 4.005 ± 0.667
1.397LysPhe: 1.397 ± 0.281
4.005LysGly: 4.005 ± 0.854
1.024LysHis: 1.024 ± 0.334
2.328LysIle: 2.328 ± 0.433
3.167LysLys: 3.167 ± 0.572
5.681LysLeu: 5.681 ± 0.829
1.211LysMet: 1.211 ± 0.347
1.583LysAsn: 1.583 ± 0.355
2.328LysPro: 2.328 ± 0.608
2.515LysGln: 2.515 ± 0.471
2.887LysArg: 2.887 ± 0.513
2.98LysSer: 2.98 ± 0.522
3.912LysThr: 3.912 ± 0.772
2.98LysVal: 2.98 ± 0.699
0.745LysTrp: 0.745 ± 0.22
1.49LysTyr: 1.49 ± 0.504
0.0LysXaa: 0.0 ± 0.0
Leu
10.524LeuAla: 10.524 ± 0.731
1.49LeuCys: 1.49 ± 0.429
6.147LeuAsp: 6.147 ± 0.764
5.774LeuGlu: 5.774 ± 0.792
1.77LeuPhe: 1.77 ± 0.37
5.868LeuGly: 5.868 ± 0.852
1.956LeuHis: 1.956 ± 0.459
3.167LeuIle: 3.167 ± 0.486
4.657LeuLys: 4.657 ± 0.556
7.358LeuLeu: 7.358 ± 0.874
1.118LeuMet: 1.118 ± 0.428
3.819LeuAsn: 3.819 ± 0.565
4.936LeuPro: 4.936 ± 0.811
4.657LeuGln: 4.657 ± 0.588
5.961LeuArg: 5.961 ± 0.915
4.284LeuSer: 4.284 ± 0.684
6.24LeuThr: 6.24 ± 0.672
4.657LeuVal: 4.657 ± 0.66
1.024LeuTrp: 1.024 ± 0.383
3.167LeuTyr: 3.167 ± 0.436
0.0LeuXaa: 0.0 ± 0.0
Met
2.422MetAla: 2.422 ± 0.491
0.0MetCys: 0.0 ± 0.0
1.583MetAsp: 1.583 ± 0.418
1.583MetGlu: 1.583 ± 0.458
0.466MetPhe: 0.466 ± 0.273
2.142MetGly: 2.142 ± 0.454
0.0MetHis: 0.0 ± 0.0
0.652MetIle: 0.652 ± 0.208
1.397MetLys: 1.397 ± 0.447
2.887MetLeu: 2.887 ± 0.463
0.373MetMet: 0.373 ± 0.164
1.024MetAsn: 1.024 ± 0.317
1.024MetPro: 1.024 ± 0.359
1.49MetGln: 1.49 ± 0.487
1.024MetArg: 1.024 ± 0.308
1.49MetSer: 1.49 ± 0.363
2.422MetThr: 2.422 ± 0.473
1.77MetVal: 1.77 ± 0.283
0.186MetTrp: 0.186 ± 0.15
0.373MetTyr: 0.373 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
3.819AsnAla: 3.819 ± 0.551
0.186AsnCys: 0.186 ± 0.122
1.583AsnAsp: 1.583 ± 0.424
1.583AsnGlu: 1.583 ± 0.458
0.838AsnPhe: 0.838 ± 0.255
2.98AsnGly: 2.98 ± 0.461
0.279AsnHis: 0.279 ± 0.17
1.956AsnIle: 1.956 ± 0.429
1.676AsnLys: 1.676 ± 0.387
2.515AsnLeu: 2.515 ± 0.346
0.931AsnMet: 0.931 ± 0.285
1.397AsnAsn: 1.397 ± 0.439
1.956AsnPro: 1.956 ± 0.507
1.49AsnGln: 1.49 ± 0.309
1.956AsnArg: 1.956 ± 0.413
2.328AsnSer: 2.328 ± 0.519
1.583AsnThr: 1.583 ± 0.425
1.863AsnVal: 1.863 ± 0.392
0.373AsnTrp: 0.373 ± 0.179
0.931AsnTyr: 0.931 ± 0.296
0.0AsnXaa: 0.0 ± 0.0
Pro
4.284ProAla: 4.284 ± 0.723
0.838ProCys: 0.838 ± 0.339
3.353ProAsp: 3.353 ± 0.631
4.191ProGlu: 4.191 ± 0.913
1.49ProPhe: 1.49 ± 0.434
2.515ProGly: 2.515 ± 0.536
0.745ProHis: 0.745 ± 0.252
1.77ProIle: 1.77 ± 0.38
1.863ProLys: 1.863 ± 0.445
3.167ProLeu: 3.167 ± 0.652
1.211ProMet: 1.211 ± 0.293
1.211ProAsn: 1.211 ± 0.369
1.863ProPro: 1.863 ± 0.501
2.049ProGln: 2.049 ± 0.513
1.304ProArg: 1.304 ± 0.3
2.049ProSer: 2.049 ± 0.377
2.701ProThr: 2.701 ± 0.49
3.26ProVal: 3.26 ± 0.635
0.373ProTrp: 0.373 ± 0.183
2.049ProTyr: 2.049 ± 0.635
0.0ProXaa: 0.0 ± 0.0
Gln
4.843GlnAla: 4.843 ± 0.86
0.745GlnCys: 0.745 ± 0.293
2.142GlnAsp: 2.142 ± 0.489
2.422GlnGlu: 2.422 ± 0.55
1.304GlnPhe: 1.304 ± 0.397
2.515GlnGly: 2.515 ± 0.433
0.186GlnHis: 0.186 ± 0.125
1.676GlnIle: 1.676 ± 0.391
2.794GlnLys: 2.794 ± 0.559
5.216GlnLeu: 5.216 ± 0.76
1.676GlnMet: 1.676 ± 0.303
1.77GlnAsn: 1.77 ± 0.431
2.235GlnPro: 2.235 ± 0.6
2.701GlnGln: 2.701 ± 0.459
3.725GlnArg: 3.725 ± 0.646
2.235GlnSer: 2.235 ± 0.525
3.725GlnThr: 3.725 ± 0.845
1.77GlnVal: 1.77 ± 0.37
0.745GlnTrp: 0.745 ± 0.198
1.211GlnTyr: 1.211 ± 0.402
0.0GlnXaa: 0.0 ± 0.0
Arg
5.588ArgAla: 5.588 ± 0.707
0.652ArgCys: 0.652 ± 0.3
2.794ArgAsp: 2.794 ± 0.454
3.632ArgGlu: 3.632 ± 0.454
2.328ArgPhe: 2.328 ± 0.455
3.539ArgGly: 3.539 ± 0.634
1.118ArgHis: 1.118 ± 0.335
4.005ArgIle: 4.005 ± 0.78
3.725ArgLys: 3.725 ± 0.511
5.309ArgLeu: 5.309 ± 0.769
1.024ArgMet: 1.024 ± 0.261
1.397ArgAsn: 1.397 ± 0.288
2.422ArgPro: 2.422 ± 0.491
3.073ArgGln: 3.073 ± 0.605
3.725ArgArg: 3.725 ± 0.725
2.515ArgSer: 2.515 ± 0.47
4.005ArgThr: 4.005 ± 0.617
2.98ArgVal: 2.98 ± 0.681
1.397ArgTrp: 1.397 ± 0.278
2.142ArgTyr: 2.142 ± 0.445
0.0ArgXaa: 0.0 ± 0.0
Ser
6.24SerAla: 6.24 ± 0.977
0.652SerCys: 0.652 ± 0.341
3.26SerAsp: 3.26 ± 0.633
2.887SerGlu: 2.887 ± 0.546
1.49SerPhe: 1.49 ± 0.371
5.774SerGly: 5.774 ± 0.795
1.676SerHis: 1.676 ± 0.817
3.819SerIle: 3.819 ± 0.487
3.819SerLys: 3.819 ± 0.722
4.377SerLeu: 4.377 ± 0.529
1.863SerMet: 1.863 ± 0.384
1.49SerAsn: 1.49 ± 0.321
1.49SerPro: 1.49 ± 0.354
1.49SerGln: 1.49 ± 0.434
2.794SerArg: 2.794 ± 0.569
3.167SerSer: 3.167 ± 0.595
4.471SerThr: 4.471 ± 1.007
4.005SerVal: 4.005 ± 0.604
1.397SerTrp: 1.397 ± 0.323
1.863SerTyr: 1.863 ± 0.472
0.0SerXaa: 0.0 ± 0.0
Thr
8.848ThrAla: 8.848 ± 0.874
0.373ThrCys: 0.373 ± 0.179
3.632ThrAsp: 3.632 ± 0.488
5.495ThrGlu: 5.495 ± 0.76
2.142ThrPhe: 2.142 ± 0.427
5.868ThrGly: 5.868 ± 0.764
0.745ThrHis: 0.745 ± 0.364
2.608ThrIle: 2.608 ± 0.771
3.725ThrLys: 3.725 ± 0.755
5.774ThrLeu: 5.774 ± 0.655
1.304ThrMet: 1.304 ± 0.351
1.583ThrAsn: 1.583 ± 0.334
3.26ThrPro: 3.26 ± 0.544
1.863ThrGln: 1.863 ± 0.472
2.701ThrArg: 2.701 ± 0.299
4.284ThrSer: 4.284 ± 0.698
5.961ThrThr: 5.961 ± 1.012
4.843ThrVal: 4.843 ± 0.679
0.466ThrTrp: 0.466 ± 0.2
1.49ThrTyr: 1.49 ± 0.302
0.0ThrXaa: 0.0 ± 0.0
Val
5.122ValAla: 5.122 ± 0.755
0.838ValCys: 0.838 ± 0.234
2.98ValAsp: 2.98 ± 0.472
4.377ValGlu: 4.377 ± 0.454
1.397ValPhe: 1.397 ± 0.338
3.073ValGly: 3.073 ± 0.562
0.931ValHis: 0.931 ± 0.261
2.515ValIle: 2.515 ± 0.483
3.353ValLys: 3.353 ± 0.582
5.774ValLeu: 5.774 ± 0.639
1.304ValMet: 1.304 ± 0.343
2.142ValAsn: 2.142 ± 0.365
2.794ValPro: 2.794 ± 0.551
2.235ValGln: 2.235 ± 0.425
3.539ValArg: 3.539 ± 0.603
4.284ValSer: 4.284 ± 0.737
5.216ValThr: 5.216 ± 0.729
4.005ValVal: 4.005 ± 0.611
1.118ValTrp: 1.118 ± 0.288
1.863ValTyr: 1.863 ± 0.497
0.0ValXaa: 0.0 ± 0.0
Trp
2.328TrpAla: 2.328 ± 0.389
0.559TrpCys: 0.559 ± 0.216
1.676TrpAsp: 1.676 ± 0.456
1.024TrpGlu: 1.024 ± 0.263
0.373TrpPhe: 0.373 ± 0.164
1.024TrpGly: 1.024 ± 0.386
0.373TrpHis: 0.373 ± 0.213
0.373TrpIle: 0.373 ± 0.198
0.373TrpLys: 0.373 ± 0.166
1.956TrpLeu: 1.956 ± 0.366
0.373TrpMet: 0.373 ± 0.246
0.559TrpAsn: 0.559 ± 0.213
0.279TrpPro: 0.279 ± 0.166
0.745TrpGln: 0.745 ± 0.351
1.397TrpArg: 1.397 ± 0.294
0.559TrpSer: 0.559 ± 0.227
0.931TrpThr: 0.931 ± 0.249
0.838TrpVal: 0.838 ± 0.278
0.279TrpTrp: 0.279 ± 0.151
0.279TrpTyr: 0.279 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.887TyrAla: 2.887 ± 0.563
0.279TyrCys: 0.279 ± 0.163
1.676TyrAsp: 1.676 ± 0.387
1.304TyrGlu: 1.304 ± 0.364
1.49TyrPhe: 1.49 ± 0.362
2.515TyrGly: 2.515 ± 0.522
0.838TyrHis: 0.838 ± 0.289
1.583TyrIle: 1.583 ± 0.373
2.142TyrLys: 2.142 ± 0.459
3.073TyrLeu: 3.073 ± 0.504
0.838TyrMet: 0.838 ± 0.22
1.118TyrAsn: 1.118 ± 0.319
1.211TyrPro: 1.211 ± 0.352
1.304TyrGln: 1.304 ± 0.317
1.956TyrArg: 1.956 ± 0.369
1.863TyrSer: 1.863 ± 0.413
2.515TyrThr: 2.515 ± 0.431
1.118TyrVal: 1.118 ± 0.251
0.652TyrTrp: 0.652 ± 0.237
1.024TyrTyr: 1.024 ± 0.308
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 42 proteins (10738 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski