secondary endosymbiont of Ctenarytaina eucalypti

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; aphid secondary symbionts

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J3TX58|J3TX58_9ENTR FeS assembly protein SufB OS=secondary endosymbiont of Ctenarytaina eucalypti OX=1199245 GN=A359_03040 PE=3 SV=1
MM1 pKa = 7.29FSFSIKK7 pKa = 10.46HH8 pKa = 4.65NTIDD12 pKa = 3.44IYY14 pKa = 9.24TNRR17 pKa = 11.84YY18 pKa = 8.27LLPDD22 pKa = 3.3VVYY25 pKa = 10.03AYY27 pKa = 10.99SEE29 pKa = 4.65FYY31 pKa = 11.49DD32 pKa = 3.87MTPEE36 pKa = 5.61DD37 pKa = 4.16IPDD40 pKa = 4.12DD41 pKa = 3.99FLSS44 pKa = 3.64

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J3TFM8|J3TFM8_9ENTR Elongation factor Ts OS=secondary endosymbiont of Ctenarytaina eucalypti OX=1199245 GN=tsf PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.68RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.28GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVYY45 pKa = 10.75SKK47 pKa = 11.5

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

918

0

918

243457

30

1407

265.2

29.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.638 ± 0.075

1.395 ± 0.032

4.913 ± 0.061

5.442 ± 0.079

3.813 ± 0.055

6.857 ± 0.072

2.726 ± 0.042

6.784 ± 0.066

4.773 ± 0.063

10.998 ± 0.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.843 ± 0.043

3.801 ± 0.052

4.102 ± 0.047

4.018 ± 0.045

6.416 ± 0.076

6.241 ± 0.053

5.183 ± 0.052

6.897 ± 0.066

1.156 ± 0.035

3.003 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski