Cochliobolus heterostrophus (strain C5 / ATCC 48332 / race O) (Southern corn leaf blight fungus) (Bipolaris maydis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris; Bipolaris maydis

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M2TGX7|M2TGX7_COCH5 Uncharacterized protein OS=Cochliobolus heterostrophus (strain C5 / ATCC 48332 / race O) OX=701091 GN=COCHEDRAFT_1024357 PE=4 SV=1
MM1 pKa = 6.64TAAVSTNGTPRR12 pKa = 11.84YY13 pKa = 9.49RR14 pKa = 11.84STLSSPSTEE23 pKa = 4.18SVTLASPLSPVPVSDD38 pKa = 4.33FEE40 pKa = 6.48DD41 pKa = 3.73IFTMSPIDD49 pKa = 3.74SEE51 pKa = 4.82TEE53 pKa = 3.54IEE55 pKa = 4.25TDD57 pKa = 3.36SNAAPVPMSFEE68 pKa = 4.26VPLGPLSGYY77 pKa = 10.41GGAPKK82 pKa = 9.88PVPKK86 pKa = 10.07YY87 pKa = 10.02KK88 pKa = 10.6KK89 pKa = 9.49PATRR93 pKa = 11.84LIKK96 pKa = 10.07PVSEE100 pKa = 4.19QPDD103 pKa = 3.6YY104 pKa = 11.21VYY106 pKa = 11.2SFKK109 pKa = 11.08GYY111 pKa = 10.36DD112 pKa = 3.26ADD114 pKa = 4.95ALVLTDD120 pKa = 3.54QQINSLGQAVDD131 pKa = 4.23GKK133 pKa = 10.3QHH135 pKa = 6.68DD136 pKa = 4.69AEE138 pKa = 4.56PMGQSSPLYY147 pKa = 10.83GEE149 pKa = 4.32TDD151 pKa = 3.41SDD153 pKa = 4.18DD154 pKa = 4.34SDD156 pKa = 3.95PFIYY160 pKa = 10.46DD161 pKa = 3.89HH162 pKa = 7.27IILPSSSSSSSSDD175 pKa = 3.3YY176 pKa = 11.46YY177 pKa = 11.45NAVDD181 pKa = 4.06DD182 pKa = 4.53DD183 pKa = 4.29QYY185 pKa = 11.67NASDD189 pKa = 4.14EE190 pKa = 4.38GDD192 pKa = 3.69SPSDD196 pKa = 3.4NNSFEE201 pKa = 4.8DD202 pKa = 3.91EE203 pKa = 4.36SAFNSNSDD211 pKa = 3.45DD212 pKa = 4.18DD213 pKa = 4.92AFEE216 pKa = 4.48AHH218 pKa = 6.79DD219 pKa = 4.34TNNDD223 pKa = 3.76DD224 pKa = 4.47VGSEE228 pKa = 4.19PDD230 pKa = 3.68SKK232 pKa = 10.95PPSSPSSAASGLYY245 pKa = 10.61FNDD248 pKa = 3.76EE249 pKa = 4.28ADD251 pKa = 3.85SEE253 pKa = 4.76HH254 pKa = 7.09NNDD257 pKa = 5.17DD258 pKa = 4.92DD259 pKa = 5.96KK260 pKa = 11.95DD261 pKa = 5.0SNDD264 pKa = 4.99SNDD267 pKa = 5.19DD268 pKa = 4.11DD269 pKa = 6.78DD270 pKa = 6.02NDD272 pKa = 4.63SNNDD276 pKa = 3.58NDD278 pKa = 6.14DD279 pKa = 4.6DD280 pKa = 6.13DD281 pKa = 5.55NDD283 pKa = 4.42PNRR286 pKa = 11.84SDD288 pKa = 4.16NDD290 pKa = 3.28NDD292 pKa = 3.42EE293 pKa = 5.03HH294 pKa = 8.91KK295 pKa = 11.04DD296 pKa = 3.79EE297 pKa = 4.39EE298 pKa = 4.65DD299 pKa = 3.22EE300 pKa = 4.9RR301 pKa = 11.84NNDD304 pKa = 3.33DD305 pKa = 3.72YY306 pKa = 11.88YY307 pKa = 11.65NPEE310 pKa = 4.82DD311 pKa = 4.56YY312 pKa = 11.09FIDD315 pKa = 3.78TNNYY319 pKa = 9.71NYY321 pKa = 10.83SDD323 pKa = 4.29DD324 pKa = 4.36NEE326 pKa = 4.98SGDD329 pKa = 3.87SSSRR333 pKa = 11.84CSNLDD338 pKa = 3.07SHH340 pKa = 7.06PLYY343 pKa = 10.91LPAPIYY349 pKa = 10.76AIAPYY354 pKa = 8.75LTYY357 pKa = 10.34QSPPTAPTMLPPVPHH372 pKa = 6.24NTPLALPSNAPSTDD386 pKa = 2.82PRR388 pKa = 11.84YY389 pKa = 10.43SDD391 pKa = 4.54LGTLEE396 pKa = 4.0ILHH399 pKa = 5.4PHH401 pKa = 5.97IRR403 pKa = 11.84NMIYY407 pKa = 10.7GFVLADD413 pKa = 3.61SPTATSEE420 pKa = 4.09EE421 pKa = 4.49NNTTGAQSDD430 pKa = 3.32IGVRR434 pKa = 11.84HH435 pKa = 5.15STAMADD441 pKa = 3.96RR442 pKa = 11.84NNMLLVCWAMRR453 pKa = 11.84EE454 pKa = 4.02EE455 pKa = 4.32VLDD458 pKa = 4.37FLALPFSTSGDD469 pKa = 3.6EE470 pKa = 3.97

Molecular weight: 51.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M2TGL3|M2TGL3_COCH5 Uncharacterized protein OS=Cochliobolus heterostrophus (strain C5 / ATCC 48332 / race O) OX=701091 GN=COCHEDRAFT_1117819 PE=4 SV=1
MM1 pKa = 7.53PLARR5 pKa = 11.84RR6 pKa = 11.84SAPRR10 pKa = 11.84TARR13 pKa = 11.84TTRR16 pKa = 11.84TTARR20 pKa = 11.84PSLKK24 pKa = 9.13TRR26 pKa = 11.84LMGGGRR32 pKa = 11.84NTRR35 pKa = 11.84THH37 pKa = 6.06TKK39 pKa = 9.71RR40 pKa = 11.84APAGTTTTTTTTTRR54 pKa = 11.84TTRR57 pKa = 11.84TTGGRR62 pKa = 11.84HH63 pKa = 3.76GHH65 pKa = 6.51AAPVHH70 pKa = 4.63HH71 pKa = 7.34HH72 pKa = 6.34KK73 pKa = 10.63RR74 pKa = 11.84HH75 pKa = 4.81ATMGDD80 pKa = 3.45KK81 pKa = 10.91VSGMMMKK88 pKa = 10.48LRR90 pKa = 11.84GSLTRR95 pKa = 11.84RR96 pKa = 11.84PGLKK100 pKa = 10.1AAGTRR105 pKa = 11.84RR106 pKa = 11.84MHH108 pKa = 5.92GTDD111 pKa = 2.72GRR113 pKa = 11.84NARR116 pKa = 11.84RR117 pKa = 11.84VYY119 pKa = 10.89

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12852

5

12857

5528821

49

7213

430.0

47.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.763 ± 0.019

1.31 ± 0.009

5.583 ± 0.016

6.064 ± 0.021

3.695 ± 0.014

6.713 ± 0.021

2.483 ± 0.01

4.914 ± 0.016

5.1 ± 0.022

8.697 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.257 ± 0.008

3.778 ± 0.011

6.069 ± 0.025

4.15 ± 0.018

5.962 ± 0.019

8.044 ± 0.026

6.041 ± 0.014

6.043 ± 0.016

1.484 ± 0.009

2.85 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski