Pseudonocardia sp. MH-G8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; unclassified Pseudonocardia

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9083 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A263DA36|A0A263DA36_9PSEU Transcriptional regulator OS=Pseudonocardia sp. MH-G8 OX=1854588 GN=CFP66_46865 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84HH3 pKa = 5.74GSRR6 pKa = 11.84ARR8 pKa = 11.84LAAVGVAASGALLLAGCGAANEE30 pKa = 4.34TAPPAGGGDD39 pKa = 3.68SAPTSDD45 pKa = 3.95VAGTIAGAGATSQQAAMEE63 pKa = 3.9AWIAGFTSIAPDD75 pKa = 3.13ATVNYY80 pKa = 9.96DD81 pKa = 3.42PTGSGAGRR89 pKa = 11.84TQFTAGAVQFAGSDD103 pKa = 3.5SLLDD107 pKa = 3.9EE108 pKa = 5.0EE109 pKa = 5.04EE110 pKa = 4.86LPAAQQICGGPDD122 pKa = 2.98NVVQMPLYY130 pKa = 9.17ISPIAVVFNLEE141 pKa = 4.07GVDD144 pKa = 4.04NIQMSPATIAKK155 pKa = 9.08VFNRR159 pKa = 11.84QITTWNDD166 pKa = 2.96PAIAAEE172 pKa = 4.14NPGVQLPATAITPVNRR188 pKa = 11.84ADD190 pKa = 3.86EE191 pKa = 4.58SGTTEE196 pKa = 4.28NFTEE200 pKa = 4.28YY201 pKa = 10.76LAATAAADD209 pKa = 3.88WPHH212 pKa = 6.58EE213 pKa = 4.27PDD215 pKa = 4.5GNWPVQGGEE224 pKa = 3.99AAQGTSGVVGAVQAGQGAIGYY245 pKa = 9.96ADD247 pKa = 4.46LSQAGEE253 pKa = 4.28LGTVDD258 pKa = 3.58VQVGDD263 pKa = 4.51GYY265 pKa = 11.74VDD267 pKa = 3.74PTPEE271 pKa = 3.25AAAAVVEE278 pKa = 4.16SSPRR282 pKa = 11.84VEE284 pKa = 4.76GQGQYY289 pKa = 11.24NFGVEE294 pKa = 4.26LARR297 pKa = 11.84DD298 pKa = 3.49STTAGTYY305 pKa = 9.59PVVLVSYY312 pKa = 8.72TIACTQYY319 pKa = 11.18SDD321 pKa = 3.94PAQADD326 pKa = 3.47AVRR329 pKa = 11.84AFLSYY334 pKa = 9.38MASEE338 pKa = 4.94EE339 pKa = 4.33GQSAASANAGNAPISASQRR358 pKa = 11.84STFQPAIDD366 pKa = 5.36AIAAAGG372 pKa = 3.41

Molecular weight: 37.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A263DUS5|A0A263DUS5_9PSEU Amine oxidase OS=Pseudonocardia sp. MH-G8 OX=1854588 GN=CFP66_17770 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 5.69GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84VKK40 pKa = 10.32GRR42 pKa = 11.84QRR44 pKa = 11.84LSAA47 pKa = 4.04

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9083

0

9083

2994516

31

15627

329.7

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.389 ± 0.042

0.747 ± 0.008

6.027 ± 0.023

5.425 ± 0.022

2.757 ± 0.021

9.597 ± 0.028

2.256 ± 0.014

3.199 ± 0.022

1.243 ± 0.018

10.597 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.719 ± 0.012

1.514 ± 0.011

6.181 ± 0.026

2.641 ± 0.013

8.375 ± 0.033

4.811 ± 0.024

5.795 ± 0.016

9.466 ± 0.03

1.466 ± 0.01

1.795 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski